WRN Protein, Human (His)
WRN Protein, Human (His) is the recombinant human-derived WRN protein, expressed by E. coli, with N-His tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
WRN Protein, Human (His) is the recombinant human-derived WRN protein, expressed by E. coli, with N-His tag.
WRN is a multifunctional enzyme with magnesium and ATP-dependent 3'-5' DNA helicase activity on partially duplex substrates. It also exhibits 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang but has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA. Helicase activity is most efficient with (d)ATP, while (d)CTP can substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3). WRN preferentially binds DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions, and may play a crucial role in dissociating joint DNA molecules arising from homologous recombination, stalled replication forks, or DNA repair. It alleviates stalling of DNA polymerases at DNA lesion sites and contributes to the formation of DNA replication focal centers by stably associating with foci elements to generate binding sites for RP-A (by similar). WRN is involved in double-strand break repair after gamma-irradiation and unwinds certain G-quadruplex DNA (d(CGG)n tracts), with unwinding occurring bidirectionally (5'-3' and 3'-5') and requiring a short single-stranded tail. d(CGG)n tracts tend to form tetraplex structures, while other G-rich substrates from telomeric or IgG switch sequences are not unwound. Depletion of WRN leads to chromosomal breaks and genome instability.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-His
-
Accession
Q14191 (S2-I333)
-
Gene ID7486
-
Molecular Construction
-
N-term
-
His
-
WRN (S2-I333)
Accession # Q14191 -
C-term
-
-
Protein Length
Partial
-
Synonyms
WRN; WRN RecQ Like Helicase; RECQL2; RECQ3; Bifunctional 3'-5' Exonuclease/ATP‑Dependent Helicase WRN; Werner Syndrome RecQ Like Helicase; DNA Helicase, RecQ‑Like Type 3; Werner Syndrome Protein; RecQ Protein‑Like 2; Werner Syndrome ATP‑Dependent Helicase
-
AA Sequence
SEKKLETTAQQRKCPEWMNVQNKRCAVEERKACVRKSVFEDDLPFLEFTGSIVYSYDASDCSFLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAINKEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMIIGSTNIETELRPSNNLNLLSFEDSTTGGVQQKQI
-
Predicted Molecular Mass
39.9 kDa
-
Molecular Weight
Approximately 40-43 kDa, based on SDS-PAGE under reducing conditions.
-
Structure/Form
Monomer
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
