XRCC6 Protein, Human (Flag)
Based on 1 Customer Validation
XRCC6 Protein, Human (Flag) is the recombinant human-derived XRCC6, expressed by E. coli , with Flag labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
XRCC6 Protein, Human (Flag) is the recombinant human-derived XRCC6, expressed by E. coli , with Flag labeled tag. ,
Background
Ku70 is a single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA, participating in double-strand break repair and V(D)J recombination. It is also involved in chromosome translocation, preferentially binding to fork-like ends of double-stranded DNA in a cell cycle-dependent manner and working in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer recognizes and binds to broken DNA ends, protecting them from further resection, and regulates DNA-PK complex activity by increasing PRKDC's affinity to DNA 100-fold. Ku70 may also function as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), cleaning abasic site termini. It acts as a negative transcriptional regulator with APEX1 and activates osteocalcin expression in association with NAA15. Additionally, Ku70 modulates DNA virus-mediated innate immune response by forming the HDP-RNP complex and suppresses apoptosis by interacting with BAX.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-Flag
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Accession
P12956-1 (M1-D609)
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Gene ID2547
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Protein Length
Full Length of Isoform-1
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Synonyms
XRCC6; X-Ray Repair Cross Complementing 6; G22P1; X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6; X-Ray Repair Cross-Complementing Protein 6; ATP-Dependent DNA Helicase 2 Subunit 1; 5'-Deoxyribose-5-Phosphate Lyase Ku70; DNA Repair Protei
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AA Sequence
MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD
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Predicted Molecular Mass
70.9 kDa
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES , pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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