XRCC6 Protein, Human (Flag)

XRCC6 Protein, Human (Flag) is the recombinant human-derived XRCC6, expressed by E. coli , with Flag labeled tag. ,

Ku70 is a single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA, participating in double-strand break repair and V(D)J recombination. It is also involved in chromosome translocation, preferentially binding to fork-like ends of double-stranded DNA in a cell cycle-dependent manner and working in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer recognizes and binds to broken DNA ends, protecting them from further resection, and regulates DNA-PK complex activity by increasing PRKDC's affinity to DNA 100-fold. Ku70 may also function as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), cleaning abasic site termini. It acts as a negative transcriptional regulator with APEX1 and activates osteocalcin expression in association with NAA15. Additionally, Ku70 modulates DNA virus-mediated innate immune response by forming the HDP-RNP complex and suppresses apoptosis by interacting with BAX.

Human

E. coli

C-Flag

P12956-1 (M1-D609)

2547

Full Length of Isoform-1

MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD

≥ 80%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES , pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.