Ribonuclease T1, Aspergillus oryzae
Based on 1 Customer Validation
Ribonulease T1, Aspergillus oryzae (Rnase T1), is commonly used in biochemical research. Ribonuclease T1 is an endonuclease that can specifically degrade single stranded RNA. Ribonuclease T1 can form nucleoside 2 ', 3 '-cyclic phosphoric acid intermediates to cut the phosphodiester bond between 3' -guanosine residues and adjacent nucleoside 5 '-OH groups to produce 3' -GMP terminal oligonucleotides.
For research use only. We do not sell to patients.
- CAS No.: 9026-12-4
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
Product Information
Molecular weight: 11.2 kDa monomer
Inhibitors: metal ions Mg2+, Ca2+, Zn2+, Fe2+, Cu2+ (100 mM MgCl2 has an inhibition rate of about 40%, 10 mM CaCl2 has an inhibition rate of about 30%); mononucleotides (2’-GMP, 3’-GMP, etc.); guanylate-2’,5’-guanosine is a specific inhibitor
Storage solution: 50 mM Tris-HCl (pH 7.4), 50% (v/v) glycerol
Digestion of RNA
It is recommended to add 0.2 μL RNase T1 per μg RNA, dissolved in 50 mM Tris-HCl (pH 7.5) and 1 mM EDTA. Incubate at 37°C for 30 min.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.1.27.3
≥10000 U/mL
≥300000 U/mg protein
One unit is defined as the amount of enzyme that will hydrolyse yeast RNA solubilised for 15 min at 37°C and pH 7.5 to increase its absorbance at 260 nm by 1.0.
Chemical Information
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CAS No. 9026-12-4
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Ribonuclease T1, Aspergillus oryzae]
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Synonyms
Rnase T1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ribonuclease T1, Aspergillus oryzae Related Classifications
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