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Sambucus Nigra Lectin (SNA) (Biotinylated) is a terminal sialic acid (SA) residue-binding lectin with biotinylation modification. Sambucus Nigra Lectin (Biotinylated) binds specifically to terminal sialic acid residues on captured IgG and its subclasses .
Phaseolus Vulgaris Leucoagglutinin (PHA-L) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Phaseolus Vulgaris Leucoagglutinin (PHA-L) Biotinylated is a biological material or organic compound that can be used in life science research .
Biocytin hydrazide is a long-chain biotin-containing hydrazide, serving as a biotin-derived active coupling reagent, selective labeling agent and biotinylating reagent. Biocytin hydrazide targets aldehyde, carboxyl, amino and guanidino groups to biotinylate glycoconjugates, K99 lectin, peptides and proteins through its hydrazide moiety, and achieves nonradioactive detection of sialic acid- and galactose-containing glycoconjugates via the avidin–biotin system .
Maackia amurensis Lectin (MAA/MAL II)-Biotinylated is a plant lectin modified by biotin. Maackia amurensis Lectin (MAA/MAL II)-Biotinylated has the activity to recognize specific sugar structures, specifically the alpha-2, 3-linked sialic acid (HY-I0400). Maackia amurensis Lectin (MAA/MAL II)-Biotinylated has a very high affinity with avidin or streptavidin and this interaction can be used to fix it to solid surfaces or bind it to other molecules. Maackia amurensis Lectin (MAA/MAL II)-Biotinylated can be used to isolate and purify proteins or other molecules with specific sugar chain structures in affinity chromatography as well as for disease marker discovery and cancer research .
Maackia Amurensis Lectin II (MAL II) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Maackia Amurensis Lectin II (MAL II) Biotinylated is a biological material or organic compound that can be used in life science research .
Ulex Europaeus Agglutinin I Biotinylated (UEA I Biotinylated) is a biotinylatedlectin that binds to α1,2-fucosylated proteins and cell-surface glycoconjugates containing α-L-fucose. Ulex Europaeus Agglutinin I Biotinylated serves as a histochemical marker for endothelial cells .
Peanut Agglutinin (PNA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Peanut Agglutinin (PNA) Biotinylated is a biological material or organic compound that can be used in life science research .
Lens Culinaris Agglutinin (LCA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Lens Culinaris Agglutinin (LCA) Biotinylated is a biological material or organic compound that can be used in life science research .
Maackia Amurensis Lectin I Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Maackia Amurensis Lectin I Biotinylated is a biological material or organic compound that can be used in life science research .
Lotus Tetragonolobus Lectin (LTL) (Biotinylated) is a biotinylated derivative of Lotus Tetragonolobus Lectin (HY-NP070), with both sugar-binding specificity and biotin labeling. Lotus Tetragonolobus Lectin (Biotinylated) is used to detect proteins, glycoproteins and lectins on nitrocellulose membranes .
Ricinus Communis Agglutinin I (RCA I) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Ricinus Communis Agglutinin I (RCA I) Biotinylated is a biological material or organic compound that can be used in life science research .
Datura Stramonium Lectin (DSL，Biotinylated) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Datura Stramonium Lectin (DSL，Biotinylated) is a biological material or organic compound that can be used in life science research .
Aleuria Aurantia Lectin (AAL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Aleuria Aurantia Lectin (AAL) Biotinylated is a biological material or organic compound that can be used in life science research .
Soybean Agglutinin (SBA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Soybean Agglutinin (SBA) Biotinylated is a biological material or organic compound that can be used in life science research .
Succinylated Wheat Germ Agglutinin (WGA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Succinylated Wheat Germ Agglutinin (WGA) Biotinylated is a biological material or organic compound that can be used in life science research .
Griffonia Simplicifolia Lectin I Isolectin B4 (GSL I-B4) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Griffonia Simplicifolia Lectin I Isolectin B4 (GSL I-B4) Biotinylated is a biological material or organic compound that can be used in life science research .
Galanthus Nivalis LectinBiotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Galanthus Nivalis LectinBiotinylated is a biological material or organic compound that can be used in life science research .
Vicia Villosa Lectin (VVL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Vicia Villosa Lectin (VVL) Biotinylated is a biological material or organic compound that can be used in life science research .
Erythrina Cristagalli Lectin (ECL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Erythrina Cristagalli Lectin (ECL) Biotinylated is a biological material or organic compound that can be used in life science research .
Wisteria Floribunda Lectin (WFL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Wisteria Floribunda Lectin (WFL) Biotinylated is a biological material or organic compound that can be used in life science research .
Narcissus Pseudonarcissus (Daffodil) LectinBiotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Narcissus Pseudonarcissus (Daffodil) LectinBiotinylated is a biological material or organic compound that can be used in life science research .
Solanum Tuberosum (Potato) Lectin (STL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Solanum Tuberosum (Potato) Lectin (STL) Biotinylated is a biological material or organic compound that can be used in life science research .
Griffonia Simplicifolia Lectin II Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Griffonia Simplicifolia Lectin II Biotinylated is a biological material or organic compound that can be used in life science research .
Griffonia Simplicifolia Lectin I Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Griffonia Simplicifolia Lectin I Biotinylated is a biological material or organic compound that can be used in life science research .
Wheat Germ Agglutinin (WGA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Wheat Germ Agglutinin (WGA) Biotinylated is a biological material or organic compound that can be used in life science research .
Dolichos Biflorus Agglutinin (DBA，Biotinylated) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Dolichos Biflorus Agglutinin (DBA，Biotinylated) is a biological material or organic compound that can be used in life science research .
Pisum Sativum Agglutinin (PSA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Pisum Sativum Agglutinin (PSA) Biotinylated is a biological material or organic compound that can be used in life science research .
Maackia amurensis Lectin (MAA/MAL II)-Biotinylated is a plant lectin modified by biotin. Maackia amurensis Lectin (MAA/MAL II)-Biotinylated has the activity to recognize specific sugar structures, specifically the alpha-2, 3-linked sialic acid (HY-I0400). Maackia amurensis Lectin (MAA/MAL II)-Biotinylated has a very high affinity with avidin or streptavidin and this interaction can be used to fix it to solid surfaces or bind it to other molecules. Maackia amurensis Lectin (MAA/MAL II)-Biotinylated can be used to isolate and purify proteins or other molecules with specific sugar chain structures in affinity chromatography as well as for disease marker discovery and cancer research .
Sambucus Nigra Lectin (SNA) (Biotinylated) is a terminal sialic acid (SA) residue-binding lectin with biotinylation modification. Sambucus Nigra Lectin (Biotinylated) binds specifically to terminal sialic acid residues on captured IgG and its subclasses .
Phaseolus Vulgaris Leucoagglutinin (PHA-L) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Phaseolus Vulgaris Leucoagglutinin (PHA-L) Biotinylated is a biological material or organic compound that can be used in life science research .
Maackia Amurensis Lectin II (MAL II) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Maackia Amurensis Lectin II (MAL II) Biotinylated is a biological material or organic compound that can be used in life science research .
Ulex Europaeus Agglutinin I Biotinylated (UEA I Biotinylated) is a biotinylatedlectin that binds to α1,2-fucosylated proteins and cell-surface glycoconjugates containing α-L-fucose. Ulex Europaeus Agglutinin I Biotinylated serves as a histochemical marker for endothelial cells .
Peanut Agglutinin (PNA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Peanut Agglutinin (PNA) Biotinylated is a biological material or organic compound that can be used in life science research .
Lens Culinaris Agglutinin (LCA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Lens Culinaris Agglutinin (LCA) Biotinylated is a biological material or organic compound that can be used in life science research .
Maackia Amurensis Lectin I Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Maackia Amurensis Lectin I Biotinylated is a biological material or organic compound that can be used in life science research .
Lotus Tetragonolobus Lectin (LTL) (Biotinylated) is a biotinylated derivative of Lotus Tetragonolobus Lectin (HY-NP070), with both sugar-binding specificity and biotin labeling. Lotus Tetragonolobus Lectin (Biotinylated) is used to detect proteins, glycoproteins and lectins on nitrocellulose membranes .
Ricinus Communis Agglutinin I (RCA I) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Ricinus Communis Agglutinin I (RCA I) Biotinylated is a biological material or organic compound that can be used in life science research .
Datura Stramonium Lectin (DSL，Biotinylated) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Datura Stramonium Lectin (DSL，Biotinylated) is a biological material or organic compound that can be used in life science research .
Aleuria Aurantia Lectin (AAL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Aleuria Aurantia Lectin (AAL) Biotinylated is a biological material or organic compound that can be used in life science research .
Soybean Agglutinin (SBA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Soybean Agglutinin (SBA) Biotinylated is a biological material or organic compound that can be used in life science research .
Succinylated Wheat Germ Agglutinin (WGA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Succinylated Wheat Germ Agglutinin (WGA) Biotinylated is a biological material or organic compound that can be used in life science research .
Griffonia Simplicifolia Lectin I Isolectin B4 (GSL I-B4) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Griffonia Simplicifolia Lectin I Isolectin B4 (GSL I-B4) Biotinylated is a biological material or organic compound that can be used in life science research .
Galanthus Nivalis LectinBiotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Galanthus Nivalis LectinBiotinylated is a biological material or organic compound that can be used in life science research .
Vicia Villosa Lectin (VVL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Vicia Villosa Lectin (VVL) Biotinylated is a biological material or organic compound that can be used in life science research .
Erythrina Cristagalli Lectin (ECL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Erythrina Cristagalli Lectin (ECL) Biotinylated is a biological material or organic compound that can be used in life science research .
Wisteria Floribunda Lectin (WFL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Wisteria Floribunda Lectin (WFL) Biotinylated is a biological material or organic compound that can be used in life science research .
Narcissus Pseudonarcissus (Daffodil) LectinBiotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Narcissus Pseudonarcissus (Daffodil) LectinBiotinylated is a biological material or organic compound that can be used in life science research .
Solanum Tuberosum (Potato) Lectin (STL) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Solanum Tuberosum (Potato) Lectin (STL) Biotinylated is a biological material or organic compound that can be used in life science research .
Griffonia Simplicifolia Lectin II Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Griffonia Simplicifolia Lectin II Biotinylated is a biological material or organic compound that can be used in life science research .
Griffonia Simplicifolia Lectin I Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.). Griffonia Simplicifolia Lectin I Biotinylated is a biological material or organic compound that can be used in life science research .
Wheat Germ Agglutinin (WGA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Wheat Germ Agglutinin (WGA) Biotinylated is a biological material or organic compound that can be used in life science research .
Dolichos Biflorus Agglutinin (DBA，Biotinylated) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Dolichos Biflorus Agglutinin (DBA，Biotinylated) is a biological material or organic compound that can be used in life science research .
Pisum Sativum Agglutinin (PSA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Pisum Sativum Agglutinin (PSA) Biotinylated is a biological material or organic compound that can be used in life science research .
MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.
CLEC5A/MDL-1 Protein positively regulates osteoclastogenesis and acts as a cell surface receptor signaling via TYROBP. Its expression relies on the TYROBP interaction and it plays a role in regulating inflammatory responses. CLEC5A/TYROBP/HCST complex formation highlights its intricate involvement in immune and inflammatory processes. CLEC5A/MDL-1 Protein, Mouse (Biotinylated, HEK293, His, Avi) is the recombinant mouse-derived CLEC5A/MDL-1 protein, expressed by HEK293, with N-Avi and N-His labeled tag.
The Siglec-15 protein plays a critical role in cellular interactions, selectively binding to sialylated glycoproteins with affinity for sialic acid residues. Binding to TYROBP and HCST suggests involvement in complex signaling pathways. Siglec-15 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived Siglec-15 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag.
Siglec-10 protein is thought to be a putative adhesion molecule that mediates sialic acid-dependent cell binding, preferentially selecting α-2,3- or α-2,6-linked sialic acid. Its sialic acid recognition site may be masked by cis interactions. Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived Siglec-10 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag.
The Siglec-15 protein plays a critical role in cellular interactions, selectively binding to sialylated glycoproteins with affinity for sialic acid residues. Binding to TYROBP and HCST suggests involvement in complex signaling pathways. Siglec-15 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Siglec-15 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
ASGR1/ASGPR1 mediates endocytosis of desialylated glycoproteins, recognizing galactose and N-acetylgalactosamine units. Ligand binding leads to internalization and sorting organelle transport, followed by receptor return to the cell membrane. ASGR1/ASGPR1 interacts with LASS2. ASGR1/ASGPR1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived ASGR1/ASGPR1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
ASGR1/ASGPR1 proteins play a crucial role in cellular processes by mediating endocytosis of desialylated plasma glycoproteins and recognizing terminal galactose and N-acetylgalactosamine. It promotes ligand internalization and formation of complexes that are transported to sorting organelles. ASGR1/ASGPR1 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived ASGR1/ASGPR1 protein, expressed by HEK293 , with N-His labeled tag.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
MedchemExpress Validation 03
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
MedchemExpress Validation 04
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
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