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Pathways Recommended:	Metabolic Enzyme/Protease

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competitive enzyme immunoassay

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Cholinesterase (ChE) (1) TGF-beta/Smad (1) TGF-β Receptor (1)
By Research Areas:	Inflammation/Immunology (1)

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Targets Recommended: Peroxidase Enzyme E1/E2/E3 Enzyme Carnosine-cleaving Enzyme Proteolytic Enzyme Endothelin-Converting Enzyme (ECE) Angiotensin-converting Enzyme (ACE) IRE1 NEDD8-activating Enzyme IDE TET Protein

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-131768

2'-O-Succinyl-cAMP	Cholinesterase (ChE)	Others
2'-O-Succinyl-cAMP is a cAMP analog that can be covalently coupled to acetylcholinesterase. 2'-O-Succinyl-cAMP conjugate has been used as tracers in a classical heterogeneous competitive enzyme immunoassay allowing the determination of cAMP .

HY-N17510

Regiafuran A	Others	Inflammation/Immunology
Regiafuran A is a prenylated 2-arylbenzofuran derivative and free radical scavenger (IC₅₀=1.9 μg/mL). Regiafuran A is isolated from the stem bark of Chlorophora regia. In a competitive enzyme immunoassay for PGE₂, Regiafuran A shows no anti-inflammatory potential .

HY-N17639

Regiafuran C	TGF-beta/Smad TGF-β Receptor	Others
Regiafuran C is a compound with both TGF-βRI inhibitory and anti-fibrotic activities. Regiafuran C effectively inhibits renal fibrosis by binding to TGF-βRI and interfering with the TGF-β/Smad and Wnt/β-catenin signaling pathways. Regiafuran C also exhibits free radical scavenging activity, but shows no anti-inflammatory activity in PGE2 competitive enzyme immunoassays. The ability of Regiafuran C to specifically block the Smad3-TGF-βRII interaction and inhibit fibrotic processes allows its use in renal fibrosis research .

Cat. No.	Product Name	Category	Target	Chemical Structure

HY-N17510

Regiafuran A	Chlorophora regia A.Chev Structural Classification Phenols Plants Moraceae Source Classification	Others
Regiafuran A is a prenylated 2-arylbenzofuran derivative and free radical scavenger (IC₅₀=1.9 μg/mL). Regiafuran A is isolated from the stem bark of Chlorophora regia. In a competitive enzyme immunoassay for PGE₂, Regiafuran A shows no anti-inflammatory potential .

HY-N17639

Regiafuran C	Structural Classification Phenols Polyphenols Plants Moraceae Morus alba Linn. Source Classification	TGF-beta/Smad TGF-β Receptor
Regiafuran C is a compound with both TGF-βRI inhibitory and anti-fibrotic activities. Regiafuran C effectively inhibits renal fibrosis by binding to TGF-βRI and interfering with the TGF-β/Smad and Wnt/β-catenin signaling pathways. Regiafuran C also exhibits free radical scavenging activity, but shows no anti-inflammatory activity in PGE2 competitive enzyme immunoassays. The ability of Regiafuran C to specifically block the Smad3-TGF-βRII interaction and inhibit fibrotic processes allows its use in renal fibrosis research .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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competitive enzyme immunoassay

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