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Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!2025-02-06
3 Results for "
contact quenching
" in MedChemExpress (MCE) Product Catalog:
Products (3)
3 Results for "contact quenching" in MCE Product Catalog:
Cat. No.: HY-NP229
Cat. No.: HY-D3319
Cat. No.: HY-D3416
Synonyms: TAMRA-2,4-dinitroaniline
Target:
Research Areas:
TMR-DN (TAMRA-2,4-dinitroaniline) is a fluorescent probe for RNA imaging that consists of a covalently coupled fluorophore (5-carboxy tetramethylrhodamine (TMR)) and quencher (dinitroaniline (DN)). The Kd value of TMR-DN with SRB-2 is 35 nM, and its Kd value with RhoBAST is approximately 30 nM. TMR-DN has a contact quenching function and forms a non-fluorescent intramolecular ground-state dimer in the free state; the excitation/emission wavelengths of the free probe are Ex/Em = 557/579 nm, those of the complex formed by binding to SRB-2 are Ex/Em = 561/587 nm, and those of the probe bound to the DNB aptamer are Ex/Em = 555/582 nm. TMR-DN reduces non-specific binding to genomic nucleic acids via negatively charged carboxyl-functionalized aromatic rings. Fluorescence dequenching occurs when TMR-DN specifically binds to SRB-2, RhoBAST or the DNB aptamer, among which the binding of RhoBAST requires Mg 2+ for proper folding .
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Protocols
Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!A suitable model is crucial in drug screening experiments. Organs can mimic the three-dimensional functional structure of internal organs, have similar spatial organization to corresponding organs, maintain some key characteristics, and reproduce some physiological functions. They are widely used for modeling and personalized drug screening physiological functions.2025-02-06
