Succinylated Wheat Germ Agglutinin (Fluorescein)  (Synonyms: WGA (Fluorescein))

Succinylated Wheat Germ Agglutinin (Fluorescein) (WGA (Fluorescein)) is a fluorescent lectin that acts as a probe to specifically bind biomolecules. Succinylated Wheat Germ Agglutinin (Fluorescein) binds to cell surface glycoconjugates containing N-acetylglucosamine. Succinylated Wheat Germ Agglutinin (Fluorescein) is a selective agglutinating agent targeting specific red blood cells, thymocytes, splenocytes and lymphocytes. Succinylated Wheat Germ Agglutinin (Fluorescein) binds to fibroblasts and lymphocytes via dual binding sites in a temperature-dependent, saturable manner. Succinylated Wheat Germ Agglutinin (Fluorescein) can be used for quantitative studies of cell surface receptor glycoconjugates (Ex=495 nm, Em=515 nm)^[1][2][3].

Succinylated Wheat Germ Agglutinin (Fluorescein) (100 μg, 1 mg/mL, 0.5 mg/mL, 60 μg/mL; 1.5 h) is an acidic protein with an isoelectric point (pI) of 4.0. Its solubility under neutral pH conditions is 100-fold higher than that of unmodified wheat germ agglutinin. It maintains a stable dimeric state even at a pH as low as 4.8, and possesses completely identical light absorption properties to unmodified wheat germ agglutinin (HY-NP091)^[1].
Succinylated Wheat Germ Agglutinin (Fluorescein) retains the ability to bind to ligands containing N-acetylglucosamine^[1].
Succinylated Wheat Germ Agglutinin (Fluorescein) (2-500 μg/mL, 2.5 mM, 0.03 mM, 50 mM; 1 h) retains agglutinating activity against rabbit red blood cells, mouse thymocytes, mouse splenocytes, and A⁺ human red blood cells even at the lowest concentration of 2 μg/mL (rabbit- and mouse-derived cells) or 10 μg/mL (type A-positive human red blood cells), but fails to agglutinate horse red blood cells even at a concentration as high as 500 μg/mL^[1].
Succinylated Wheat Germ Agglutinin (Fluorescein) exhibits almost identical fluorescence and phosphorescence responses to N-acetylglucosamine oligosaccharides as unmodified wheat germ agglutinin^[1].
Succinylated Wheat Germ Agglutinin (Fluorescein) binds to both untreated and neuraminidase-treated BHK 21 C 13 cells; it binds to rat splenic lymphocytes non-cooperatively; and it binds to rat splenic lymphocytes with high affinity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Succinylated Wheat Germ Agglutinin (Fluorescein) binds to thymocytes of BALB/c mice, with a single-cell binding capacity of 2.1×10⁶; Scatchard analysis identifies two types of binding sites with high and low affinities; and its GlcNAc oligosaccharide-binding activity is slightly lower than that of native lectin^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

[Succinylated Wheat Germ Agglutinin (Fluorescein)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Monsigny M, et al. Properties of Succinylated Wheat-Germ Agglutinin. Eur J Biochem. 1979 Jul;98(1):39-45.

  [2]. Monsigny M, et al. Sugar-lectin interactions: how does wheat-germ agglutinin bind sialoglycoconjugates?. Eur J Biochem. 1980;104(1):147-153.

  [3]. Moullier P, et al. Comparative binding of wheat germ agglutinin and its succinylated form on lymphocytes. Eur J Biochem. 1986;161(1):197-204.