Surfactin C1

Cat. No.: HY-W588250: FAQs
Data Sheet SDS Handling Instructions

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Surfactin C1 is an amphiphilic biosurfactants. Surfactin C1 inhibits leukemic cell (HL-60) adhesion to human umbilical vein endothelial cells (HUVEC). Surfactin C1 inhibits adhesion melecules expression, such as ICAM-1 and VCAM-1.

For research use only. We do not sell to patients.

Surfactin C1 Chemical Structure

CAS No. : 24730-31-2

Size	Price	Stock
5 mg	USD 100	Ask For Quote & Lead Time
10 mg	USD 150	Ask For Quote & Lead Time

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This product is a controlled substance and not for sale in your territory.

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Surfactin C1 (0.3-10 μg/mL; 24 h) inhibits the adhesion of leukemic cell and monocyte cell to HUVEC, stimulated by LPS^[1].
Surfactin C1 (3 μg/mL; 1 h) inhibits LPS-induced expression of adhesion molecules^[1].
Surfactin C1 (3 μg/mL; 1 h) inhibits the interaction of lipid A/LBP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	HUVEC
Concentration:	0.3 μg/mL, 1 μg/mL, 3 μg/mL, 10 μg/mL
Incubation Time:	2 hr and another 4 hr with 1 μg/mL LPS
Result:	Decreased the protein level of ICAM-1 and VCAM-1. Completely inhibited the expression at 3μg/mL with no effect on E-selectin.

Cell Viability Assay^[1]

Cell Line:	HL-60, THP-1, Jurkat cells
Concentration:	0.3 μg/mL, 1 μg/mL, 3 μg/mL, 10 μg/mL, and 100 μg/mL
Incubation Time:	24 hr accompanied with LPS for viability assay; 2 hr and another 4 hr with LPS for adhesion assay
Result:	Didn’t inhibit cell viability. Inhibited cell adhesion to HUVEC with IC50s of 1.10 μg/mL, 1.45 μg/mL, and 1.43 μg/mL, respectively.

Molecular Weight

1036.34

Formula

C₅₃H₉₃N₇O₁₃

CAS No.

24730-31-2

Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (96.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9649 mL	4.8247 mL	9.6493 mL
5 mM	0.1930 mL	0.9649 mL	1.9299 mL
10 mM	0.0965 mL	0.4825 mL	0.9649 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Data Sheet (276 KB) SDS (251 KB) Handling Instructions (2659 KB)

References

[1]. Takahashi T, et al. Inhibition of lipopolysaccharide activity by a bacterial cyclic lipopeptide surfactin. J Antibiot (Tokyo). 2006 Jan;59(1):35-43. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9649 mL	4.8247 mL	9.6493 mL	24.1234 mL
	5 mM	0.1930 mL	0.9649 mL	1.9299 mL	4.8247 mL
	10 mM	0.0965 mL	0.4825 mL	0.9649 mL	2.4123 mL
	15 mM	0.0643 mL	0.3216 mL	0.6433 mL	1.6082 mL
	20 mM	0.0482 mL	0.2412 mL	0.4825 mL	1.2062 mL
	25 mM	0.0386 mL	0.1930 mL	0.3860 mL	0.9649 mL
	30 mM	0.0322 mL	0.1608 mL	0.3216 mL	0.8041 mL
	40 mM	0.0241 mL	0.1206 mL	0.2412 mL	0.6031 mL
	50 mM	0.0193 mL	0.0965 mL	0.1930 mL	0.4825 mL
	60 mM	0.0161 mL	0.0804 mL	0.1608 mL	0.4021 mL
	80 mM	0.0121 mL	0.0603 mL	0.1206 mL	0.3015 mL

Surfactin C1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Surfactin C124730-31-2Surfactin C 1Surfactin C-1IntegrinInhibitorinhibitorinhibit

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