Isatin-β-thiosemicarbazone
Based on 1 Customer Validation
Isatin-β-thiosemicarbazone is a potent anti-poxvirus agent (including monkeypox virus, orthopoxvirus, vaccinia virus, etc). Isatin-β-thiosemicarbazone also is a potent herpes simplex virus (HSV) inhibitor. Isatin-β-thiosemicarbazone exhibits
For research use only. We do not sell to patients.
- Purity: 99.58%
- CAS No.: 27830-79-1
- Formula: C9H8N4OS
- Molecular Weight:220.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 184B5 | GI50 |
60.42 μM
Compound: 9e
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Cytotoxicity against human 184B5 cells after 48 hrs by SRB assay
Cytotoxicity against human 184B5 cells after 48 hrs by SRB assay
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[PMID: 20106668] |
| KB 3-1 | IC50 |
>50 μM
Compound: 13
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Cytotoxicity against human KB-3-1 cells after 72 hrs by MTT assay
Cytotoxicity against human KB-3-1 cells after 72 hrs by MTT assay
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[PMID: 21721528] |
| KB 3-1 | IC50 |
>50 μM
Compound: 14
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Cytotoxicity against human P-gp-negative KB-3-1 cells after 72 hrs by MTT assay
Cytotoxicity against human P-gp-negative KB-3-1 cells after 72 hrs by MTT assay
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[PMID: 19397322] |
| KB-V1 | IC50 |
>50 μM
Compound: 13
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Cytotoxicity against vinblastine-selected human KBV1 cells after 72 hrs by MTT assay
Cytotoxicity against vinblastine-selected human KBV1 cells after 72 hrs by MTT assay
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[PMID: 21721528] |
| KB-V1 | IC50 |
>50 μM
Compound: 14
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Cytotoxicity against human P-glycoprotein-expressing KBV1 cells after 72 hrs by MTT assay
Cytotoxicity against human P-glycoprotein-expressing KBV1 cells after 72 hrs by MTT assay
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[PMID: 19397322] |
| MCF-10A | GI50 |
88.78 μM
Compound: 9e
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Cytotoxicity against human MCF10A cells after 48 hrs by SRB assay
Cytotoxicity against human MCF10A cells after 48 hrs by SRB assay
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[PMID: 20106668] |
| MCF7 | GI50 |
36.93 μM
Compound: 9e
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Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
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[PMID: 20106668] |
| MDA-MB-468 | GI50 |
35.45 μM
Compound: 9e
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Cytotoxicity against human MDA-MB-468 cells after 48 hrs by SRB assay
Cytotoxicity against human MDA-MB-468 cells after 48 hrs by SRB assay
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[PMID: 20106668] |
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 27830-79-1
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Appearance Solid
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Molecular Weight 220.25
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Formula C9H8N4OS
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Color Light yellow to yellow
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SMILES
O=C1NC2=C(C=CC=C2)/C1=N/NC(N)=S
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Synonyms
IBT
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 125 mg/mL (567.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.44 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Meis RJ, et al. Genetic and molecular biological characterization of a vaccinia virus gene which renders the virus dependent on isatin-beta-thiosemicarbazone (IBT). Virology. 1991 Jun;182(2):442-54. [Content Brief]
[2]. Katz E, et al. Synthesis of vaccinia virus polypeptides in the presence of isatin-beta-thiosemicarbazone. Antimicrob Agents Chemother. 1973 Jul;4(1):44-8. [Content Brief]
[3]. Iou-Jiun Kang, et al. Isatin-β-thiosemicarbazones as potent herpes simplex virus inhibitors. Bioorg Med Chem Lett. 2011 Apr 1;21(7):1948-52. [Content Brief]
[4]. William D Arndt, et al. Monkeypox virus induces the synthesis of less dsRNA than vaccinia virus, and is more resistant to the anti-poxvirus drug, IBT, than vaccinia virus. Virology. 2016 Oct;497:125-135. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.5403 mL | 22.7015 mL | 45.4030 mL | 113.5074 mL |
| 5 mM | 0.9081 mL | 4.5403 mL | 9.0806 mL | 22.7015 mL | |
| 10 mM | 0.4540 mL | 2.2701 mL | 4.5403 mL | 11.3507 mL | |
| 15 mM | 0.3027 mL | 1.5134 mL | 3.0269 mL | 7.5672 mL | |
| 20 mM | 0.2270 mL | 1.1351 mL | 2.2701 mL | 5.6754 mL | |
| 25 mM | 0.1816 mL | 0.9081 mL | 1.8161 mL | 4.5403 mL | |
| 30 mM | 0.1513 mL | 0.7567 mL | 1.5134 mL | 3.7836 mL | |
| 40 mM | 0.1135 mL | 0.5675 mL | 1.1351 mL | 2.8377 mL | |
| 50 mM | 0.0908 mL | 0.4540 mL | 0.9081 mL | 2.2701 mL | |
| 60 mM | 0.0757 mL | 0.3784 mL | 0.7567 mL | 1.8918 mL | |
| 80 mM | 0.0568 mL | 0.2838 mL | 0.5675 mL | 1.4188 mL | |
| 100 mM | 0.0454 mL | 0.2270 mL | 0.4540 mL | 1.1351 mL |
Isatin-β-thiosemicarbazone Related Classifications
HY-W291131 Related Classifications
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