Tetramethylrhodamine-5-maleimide

Name: Tetramethylrhodamine-5-maleimide
Brand: MedChemExpress
SKU: HY-D0151
Rating: 4.5 (0 reviews)

Cat. No.: HY-D0151 Purity: 89.67%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Tetramethylrhodamine-5-maleimide is a fluorescent probe with high specificity for cysteine thiol (SH) groups. Tetramethylrhodamine-5-maleimide has high sensitivity, responds rapidly to negatively charged nanoparticles, and shows a concentration-dependent decrease in fluorescence intensity, but it is susceptible to interference from humic acids.

For research use only. We do not sell to patients.

Tetramethylrhodamine-5-maleimide Chemical Structure

CAS No. : 174568-67-3

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Tetramethylrhodamine-5-maleimide (50 mM; 2 h) covalently labels the custom-engineered Ac-KKKKKKKGGGC-OH peptide with 100% efficiency under the specified conditions, yielding a purified labeled peptide with a purity of >90%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

481.50

Formula

C₂₈H₂₃N₃O₅

CAS No.

174568-67-3

Appearance

Solid

Color

Purple to purplish red

SMILES

O=C(C1=C(C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)C=C(N5C(C=CC5=O)=O)C=C1)[O-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 125 mg/mL (259.61 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0768 mL	10.3842 mL	20.7684 mL
5 mM	0.4154 mL	2.0768 mL	4.1537 mL
10 mM	0.2077 mL	1.0384 mL	2.0768 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 89.67%

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Data Sheet (278 KB) SDS (252 KB)

COA (259 KB) HNMR (193 KB) RP-HPLC (228 KB) MS (231 KB)

Handling Instructions (2659 KB)

References

[1]. Akinloye O, et al. Peptide-based fluorescence biosensors for detection/measurement of nanoparticles. Anal Bioanal Chem. 2017;409(4):903-915. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0768 mL	10.3842 mL	20.7684 mL	51.9211 mL
	5 mM	0.4154 mL	2.0768 mL	4.1537 mL	10.3842 mL
	10 mM	0.2077 mL	1.0384 mL	2.0768 mL	5.1921 mL
	15 mM	0.1385 mL	0.6923 mL	1.3846 mL	3.4614 mL
	20 mM	0.1038 mL	0.5192 mL	1.0384 mL	2.5961 mL
	25 mM	0.0831 mL	0.4154 mL	0.8307 mL	2.0768 mL
	30 mM	0.0692 mL	0.3461 mL	0.6923 mL	1.7307 mL
	40 mM	0.0519 mL	0.2596 mL	0.5192 mL	1.2980 mL
	50 mM	0.0415 mL	0.2077 mL	0.4154 mL	1.0384 mL
	60 mM	0.0346 mL	0.1731 mL	0.3461 mL	0.8654 mL
	80 mM	0.0260 mL	0.1298 mL	0.2596 mL	0.6490 mL
	100 mM	0.0208 mL	0.1038 mL	0.2077 mL	0.5192 mL