3-Methylcrotonylglycine
Based on 1 publication(s) in Google Scholar
3-Methylcrotonylglycine is an organic acid conjugate associated with leucine catabolism that inhibits respiratory chain complex II-III, mitochondrial creatine kinase, and synaptic membrane Na+, K+-ATPase. 3-Methylcrotonylglycine increases reactive oxygen species levels to mediate oxidative damage, while inhibiting mitochondrial electron transport, reducing tricarboxylic acid cycle flux, and interfering with intracellular energy transport and neuronal ion homeostasis. 3-Methylcrotonylglycine is a neurotoxic metabolite that accumulates in the metabolic pathway of 3-methylcrotonyl-CoA carboxylase deficiency. 3-Methylcrotonylglycine can be used in studies related to 3-methylcrotonyl-CoA carboxylase deficiency.
For research use only. We do not sell to patients.
- Purity: 98.67%
- CAS No.: 33008-07-0
- Formula: C7H11NO3
- Molecular Weight:157.17
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 3-Methylcrotonylglycine
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
3-Methylcrotonylglycine (3MCG) (0.1-5.0 mM; 30 min pre-incubation) dose-dependently inhibits the production of CO2 from [1-14C] acetate in cerebral cortex homogenates of 30-day-old Wistar rats; it also dose-dependently inhibits the activities of respiratory chain complex II-III, mitochondrial creatine kinase, and total creatine kinase[1].
3-Methylcrotonylglycine (0.1-5.0 mM; 30 min pre-incubation at 37°C for purified membranes, 30 min pre-incubation for homogenates) reduces the activity of synaptic Na+, K+-ATPase by up to 45% in purified synaptic membranes from 30-day-old Wistar rats, and decreases the activity of this enzyme by 40% in membranes isolated from pre-incubated cerebral cortex homogenates[1].
3-Methylcrotonylglycine (0.1-5.0 mM; 1 h at 37°C) dose-dependently increases the lipid peroxidation level by up to 90% in cerebral cortex homogenates of 30-day-old Wistar rats[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 33008-07-0
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Appearance Solid
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Molecular Weight 157.17
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Formula C7H11NO3
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Color White to off-white
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SMILES
O=C(O)CNC(/C=C(C)\C)=O
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (636.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 12.5 mg/mL (79.53 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 5 mg/mL (31.81 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 6.3625 mL | 31.8127 mL | 63.6254 mL | 159.0634 mL |
| 5 mM | 1.2725 mL | 6.3625 mL | 12.7251 mL | 31.8127 mL | |
| 10 mM | 0.6363 mL | 3.1813 mL | 6.3625 mL | 15.9063 mL | |
| 15 mM | 0.4242 mL | 2.1208 mL | 4.2417 mL | 10.6042 mL | |
| 20 mM | 0.3181 mL | 1.5906 mL | 3.1813 mL | 7.9532 mL | |
| 25 mM | 0.2545 mL | 1.2725 mL | 2.5450 mL | 6.3625 mL | |
| 30 mM | 0.2121 mL | 1.0604 mL | 2.1208 mL | 5.3021 mL | |
| 40 mM | 0.1591 mL | 0.7953 mL | 1.5906 mL | 3.9766 mL | |
| 50 mM | 0.1273 mL | 0.6363 mL | 1.2725 mL | 3.1813 mL | |
| 60 mM | 0.1060 mL | 0.5302 mL | 1.0604 mL | 2.6511 mL | |
| DMSO | 80 mM | 0.0795 mL | 0.3977 mL | 0.7953 mL | 1.9883 mL |
| 100 mM | 0.0636 mL | 0.3181 mL | 0.6363 mL | 1.5906 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
3-Methylcrotonylglycine Related Classifications
HY-113232 Related Classifications
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Keywords
- 3-Methylcrotonylglycine
- 33008-07-0
- Endogenous Metabolite
- Na+/K+ ATPase
- Mitochondrial Metabolism
- cerebral cortex
- citric acid cycle
- lipid peroxidation
- young rats
- 3-methylcrotonyl-coa carboxylase deficiency
- synaptic membrane Na+,K+-ATPase
- Wistar rats
- mitochondrial creatine kinase
- glutathione peroxidase
- respiratory chain complex II-III
- Inhibitor
- inhibitor
- inhibit