5-(Biotinamido)pentylamine
Based on 1 Customer Validation
5-(Biotinamido)pentylamine is an amine donor substrate for transglutaminase. 5-(Biotinamido)pentylamine can be used as a biotin-labeled probe to specifically participate in the calcium-dependent reaction catalyzed by TG, bind to the γ-carboxamide group of the glutamine residue in the protein, introduce the biotin tag into the target protein, and form a biotinylated protein product. 5-(Biotinamido)pentylamine can be used for the labeling, separation, and detection of TG amine receptor protein substrates.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 115416-38-1
- Formula: C15H28N4O2S
- Molecular Weight:328.47
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
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Biological Activity
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Alkyl-Chain |
5-(Biotinamido)pentylamine (5 mM; 1 h) can specifically label a variety of protein substrates in mouse, rat, and guinea pig liver extracts through the catalysis of endogenous transglutaminase (TG). Glyceraldehyde-3-phosphate dehydrogenase (38 kDa) and glutathione S-transferase (28 kDa) are identified by affinity chromatography and SDS-PAGE separation[1].
5-(Biotinamido)pentylamine (1 mM; 30 min) can label 26, 40, 55, and 60 kDa proteins in MCF-7 human breast cancer cell lysate under Ca2+-dependent conditions. The 55 kDa protein was significantly labeled in resistant cells, while the labeling was weak in sensitive cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 115416-38-1
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Appearance Solid
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Molecular Weight 328.47
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Formula C15H28N4O2S
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Color Off-white to light yellow
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SMILES
O=C(NCCCCCN)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (304.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Ikura K, et al. Identification of amine acceptor protein substrates of transglutaminase in liver extracts: use of 5-(biotinamido) pentylamine as a probe. Arch Biochem Biophys. 1998 Aug 15;356(2):280-6. [Content Brief]
[2]. Singh RN, et al. Detection of the amine acceptor protein substrates of transglutaminase with 5-(biotinamido) pentylamine. Anal Biochem. 1995 Oct 10;231(1):261-3. [Content Brief]
[3]. Ichikawa A, et al. Identification of new amine acceptor protein substrate candidates of transglutaminase in rat liver extract: use of 5-(biotinamido) pentylamine as a probe. Biosci Biotechnol Biochem. 2008 Apr;72(4):1056-62. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.0444 mL | 15.2221 mL | 30.4442 mL | 76.1105 mL |
| 5 mM | 0.6089 mL | 3.0444 mL | 6.0888 mL | 15.2221 mL | |
| 10 mM | 0.3044 mL | 1.5222 mL | 3.0444 mL | 7.6110 mL | |
| 15 mM | 0.2030 mL | 1.0148 mL | 2.0296 mL | 5.0740 mL | |
| 20 mM | 0.1522 mL | 0.7611 mL | 1.5222 mL | 3.8055 mL | |
| 25 mM | 0.1218 mL | 0.6089 mL | 1.2178 mL | 3.0444 mL | |
| 30 mM | 0.1015 mL | 0.5074 mL | 1.0148 mL | 2.5370 mL | |
| 40 mM | 0.0761 mL | 0.3806 mL | 0.7611 mL | 1.9028 mL | |
| 50 mM | 0.0609 mL | 0.3044 mL | 0.6089 mL | 1.5222 mL | |
| 60 mM | 0.0507 mL | 0.2537 mL | 0.5074 mL | 1.2685 mL | |
| 80 mM | 0.0381 mL | 0.1903 mL | 0.3806 mL | 0.9514 mL | |
| 100 mM | 0.0304 mL | 0.1522 mL | 0.3044 mL | 0.7611 mL |
5-(Biotinamido)pentylamine Related Classifications
HY-W076543 Related Classifications
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