5-HMSiR-Hoechst 

5-HMSiR-Hoechst is a DNA probe. 5-HMSiR-Hoechst comprises Hoechst 33258 and spontaneously blinking far-red hydroxymethyl silicon-rhodamine (HMSiR). 5-HMSiR-Hoechst can stain living cells. Ex/Em=640 nm/675 nm^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Stock solution preparation: Prepare a 1 mM stock solution with anhydrous DMSO and store it at -20℃.
2. Working solution preparation: Dilute the stock solution with phenol red-free DMEM/HDMEM (containing 10% FBS, 10 mM HEPES) to a final concentration of 0.1-1 μM (0.5 μM is commonly used), and prepare it fresh before use.
3. Staining: Aspirate the old medium, add complete medium containing 0.5 μM 5-HMSiR-Hoechst, and incubate at 37℃, 5% CO2 in the dark for 30-60 min.
4. Fluorescence detection: Wash twice with pre-warmed PBS, replace with fresh medium, and then perform imaging detection. The excitation wavelength is 640 nm, and the emission wavelength is 675 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

908.17

C₅₄H₅₇N₉O₃Si

2996108-30-4

Solid

Green to dark green

CN1CCN(CC1)C2=CC=C3NC(C4=CC=C5NC(C6=CC=C(C=C6)OCCNC(C7=CC8=C(C9(C%10=C([Si](C%11=C9C=CC(N(C)C)=C%11)(C)C)C=C(C=C%10)N(C)C)OC8)C=C7)=O)=NC5=C4)=NC3=C2

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Jonas Bucevičius, et al. Far-red switching DNA probes for live cell nanoscopy. Chem Commun (Camb). 2020, 56, 94.  [Content Brief]

  [2]. S. H. Ferreira, et al. A bradykinin-potentiating factor (bpf) present in the venom of bothrops jararaca. Br J Pharmacol Chemother. 1995, 24, 1.