Far-red switching DNA probes for live cell nanoscopy

  • Chem Commun (Camb). 2020 Nov 26;56(94):14797-14800. doi: 10.1039/d0cc06759h.
Jonas Bucevičius  1 ,  Tanja Gilat  1 ,  GraŽvydas Lukinavičius  1
Affiliations
  • 1. Chromatin Labeling and Imaging Group, Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. [email protected].
PMID: 33140748 DOI: 10.1039/d0cc06759h
Abstract

Herein we present DNA probes composed of Hoechst 33258 and spontaneously blinking far-red hydroxymethyl silicon-rhodamine (HMSiR). The best performing probe, 5-HMSiR-Hoechst, contains the 5'-regioisomer, shows ∼400-fold fluorescence increase upon DNA binding and is compatible with wash-free single molecule localization and 3D stimulated emission depletion microscopy of chromatin nanostructures in living cells.

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