5-HMSiR-Hoechst

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Stock solution preparation: Prepare a 1 mM stock solution with anhydrous DMSO and store it at -20℃.
2. Working solution preparation: Dilute the stock solution with phenol red-free DMEM/HDMEM (containing 10% FBS, 10 mM HEPES) to a final concentration of 0.1-1 μM (0.5 μM is commonly used), and prepare it fresh before use.
3. Staining: Aspirate the old medium, add complete medium containing 0.5 μM 5-HMSiR-Hoechst, and incubate at 37℃, 5% CO2 in the dark for 30-60 min.
4. Fluorescence detection: Wash twice with pre-warmed PBS, replace with fresh medium, and then perform imaging detection. The excitation wavelength is 640 nm, and the emission wavelength is 675 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.