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Cat. No.: HY-D3504

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5-JOE azide, CHC protected

5-JOE azide, CHC-protected, is a derivative of JOE. It features an azide group for use in click chemistry reactions (CuAAC) and a CHC (cyclohexylcarbodiimide) protecting group for stable oligonucleotide synthesis. 5-JOE azide, CHC-protected, is suitable for click chemistry-based bioconjugation (Ex/Em = 520/548 nm) .

For research use only. We do not sell to patients.

Formula: C₄₀H₄₀Cl₂N₄O₁₀
Molecular Weight:807.67

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

5-JOE azide, CHC protected Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

Chemical Information

Molecular Weight 807.67
Formula C₄₀H₄₀Cl₂N₄O₁₀
SMILES

[N-]=[N+]=NCCCNC(C1=CC=C(C(C(O2)=O)=C1)C32C(C=C4OC)=C(C(Cl)=C4OC(C5CCCCC5)=O)OC6=C3C=C(OC)C(OC(C7CCCCC7)=O)=C6Cl)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.

Purity & Documentation

Handling Instructions (2659 KB)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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