5-Nitro-1,10-phenanthroline
Based on 1 Customer Validation
5-Nitro-1,10-phenanthroline (5-NP), is a o-Phenanthroline (HY-W004544) derivative, as a mediator of glucose oxidase (GOX) with antituberculous activity. 5-Nitro-1,10-phenanthroline can be applied as redox mediators for oxidases and is suitable for the development of reagent-less biosensors and biofuel cells.
For research use only. We do not sell to patients.
- Purity: 99.66%
- CAS No.: 4199-88-6
- Formula: C12H7N3O2
- Molecular Weight:225.21
-
Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Bioactive Compound Library Max
HY-L181
-
Bioactive Compound Library
HY-L001
-
Anti-Infection Compound Library
HY-L002
-
Metabolism/Protease Compound Library
HY-L012
-
Natural Product Library
HY-L021
-
Alkaloids Library
HY-L071
-
Heterocyclic Compound Library
HY-L138
-
Anti-tuberculosis Compound Library
HY-L142
-
MCE Bioactive Compound Library
HY-L001V
-
Natural Product Library Plus
HY-L021P
-
Natural Product and Natural Product-Like Compound Library
HY-L021M
-
RO5 Drug-like Natural Product Library
HY-L200
-
High-Throughput Bioactive Compound Library
HY-L205
-
High-Throughput Natural Product Library
HY-L206
-
Natural Product Diversity Scaffold Library
HY-L245
-
Mass Spectrometry Natural Product Library
HY-L262
All Endogenous Metabolite Isoforms
More
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| THP-1 | CC50 |
>25 μM
Compound: 117
|
Cytotoxicity against human THP-1 cells assessed as reduction in cell viability treated for 96 hrs by MTT assay
Cytotoxicity against human THP-1 cells assessed as reduction in cell viability treated for 96 hrs by MTT assay
|
[PMID: 35612311] |
5-Nitro-1,10-phenanthroline (25 μM; 24 h) kills naturally resistant intracellular bacteria by inducing autophagy in THP-1 macrophages[2].
5-Nitro-1,10-phenanthroline (1x, 5x, 20x or 50x MIC, MIC=0.78 μM; 1 h) also modulates the host machinery to kill intracellular pathogens by inhibiting mycolic acid biosynthesis of Mtb[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Mtb H37Rv, M. bovis BCG and M. bovis BCG-5NP resistant strain
-
Concentration:0-12.5 μM
-
Incubation Time:24 hours
-
Result:Inhibited pathogens with MIC99 values of 0.78 μM (Mtb H37Rv), 0.78 μM (M. bovis BCG), and >12.5 μM (M. bovis BCG-5NP), respectively.
Chemical Information
-
CAS No. 4199-88-6
-
Appearance Solid
-
Molecular Weight 225.21
-
Formula C12H7N3O2
-
Color Light yellow to yellow
-
SMILES
O=[N+]([O-])C1=C2C(N=CC=C2)=C3C(C=CC=N3)=C1
-
Synonyms
5-NP
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 35 mg/mL (155.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 10 mg/mL (44.40 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (279 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[1]. Oztekin Y, et al. 1,10-Phenanthroline derivatives as mediators for glucose oxidase. Biosens Bioelectron. 2010 Sep 15;26(1):267-70. [Content Brief]
[2]. Kidwai S, et al. Dual Mechanism of Action of 5-Nitro-1,10-Phenanthroline against Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2017 Oct 24;61(11):e00969-17. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 4.4403 mL | 22.2015 mL | 44.4030 mL | 111.0075 mL |
| 5 mM | 0.8881 mL | 4.4403 mL | 8.8806 mL | 22.2015 mL | |
| 10 mM | 0.4440 mL | 2.2202 mL | 4.4403 mL | 11.1008 mL | |
| 15 mM | 0.2960 mL | 1.4801 mL | 2.9602 mL | 7.4005 mL | |
| 20 mM | 0.2220 mL | 1.1101 mL | 2.2202 mL | 5.5504 mL | |
| 25 mM | 0.1776 mL | 0.8881 mL | 1.7761 mL | 4.4403 mL | |
| 30 mM | 0.1480 mL | 0.7401 mL | 1.4801 mL | 3.7003 mL | |
| 40 mM | 0.1110 mL | 0.5550 mL | 1.1101 mL | 2.7752 mL | |
| DMSO | 50 mM | 0.0888 mL | 0.4440 mL | 0.8881 mL | 2.2202 mL |
| 60 mM | 0.0740 mL | 0.3700 mL | 0.7401 mL | 1.8501 mL | |
| 80 mM | 0.0555 mL | 0.2775 mL | 0.5550 mL | 1.3876 mL | |
| 100 mM | 0.0444 mL | 0.2220 mL | 0.4440 mL | 1.1101 mL |
5-Nitro-1,10-phenanthroline Related Classifications
HY-W004570 Related Classifications
Powered by Bioz