Cat. No.: HY-W004544

o-Phenanthroline

(Synonyms: 1,10-Phenanthroline)

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

o-Phenanthroline (1,10-Phenanthroline), a metal chelator, prevents the induction of chromosomal aberrations in streptozotocin-treated cells. o-Phenanthroline (1,10-Phenanthroline) forms a red chelate with Fe²⁺ that absorbs maximally at 510 nm. o-Phenanthroline (1,10-Phenanthroline) is also a MMP inhibitor.

For research use only. We do not sell to patients.

Purity: 99.95%
CAS No.: 66-71-7
Formula: C₁₂H₈N₂
Molecular Weight:180.21

Storage:

RT, protect from light, stored under nitrogen.

In solvent -80°C, 1 year , -20°C, 6 months

o-Phenanthroline Chemical Structure

CAS No.: 66-71-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All MMP Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	2.73 μM Compound: Phenanthroline	cytotoxic activity toward A2780 ovarian human cell line resistant (R) to cisplatin in experiment 3 cytotoxic activity toward A2780 ovarian human cell line resistant (R) to cisplatin in experiment 3	[PMID: 11000008]
A2780	IC₅₀	3.66 μM Compound: Phenanthroline	cytotoxic activity toward A2780 ovarian human cell line sensitive (S) to cisplatin in experiment 3 cytotoxic activity toward A2780 ovarian human cell line sensitive (S) to cisplatin in experiment 3	[PMID: 11000008]
COS-7	EC₅₀	3.9 μM Compound: Phe	Allosteric modulation at human CCR8 transfected in COS7 cells assessed as [3H]IP3 turnover by liquid scintillation counting analysis Allosteric modulation at human CCR8 transfected in COS7 cells assessed as [3H]IP3 turnover by liquid scintillation counting analysis	[PMID: 22957890]
COS-7	EC₅₀	4.9 μM Compound: Phe	Allosteric modulation at human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as [3H]IP3 turnover by liquid scintillation counting analysis Allosteric modulation at human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as [3H]IP3 turnover by liquid scintillation counting analysis	[PMID: 22957890]
COS-7	EC₅₀	5.9 μM Compound: Phe	Allosteric modulation at human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as {3H]IP3 turnover by liquid scintillation counting analysis Allosteric modulation at human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as {3H]IP3 turnover by liquid scintillation counting analysis	[PMID: 22957890]
COS-7	IC₅₀	53 μM Compound: Phe	Displacement of [125I]-CCL3 from human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs Displacement of [125I]-CCL3 from human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs	[PMID: 22957890]
COS-7	IC₅₀	74 μM Compound: Phe	Displacement of [125I]-CCL3 from human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs Displacement of [125I]-CCL3 from human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs	[PMID: 22957890]
NHDF	CC₅₀	70.2 μM Compound: 1,10-Phen	Cytotoxicity against NHDF cells assessed as reduction in cell viability measured after 96 hrs by MTS assay Cytotoxicity against NHDF cells assessed as reduction in cell viability measured after 96 hrs by MTS assay	[PMID: 34666274]

In Vitro

o-Phenanthroline (1,10-Phenanthroline, 1mM) inhibits autolysis of sea cucumber body wall^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 66-71-7
Appearance Solid
Molecular Weight 180.21
Formula C₁₂H₈N₂
Color White to off-white
SMILES

C12=C(N=CC=C3)C3=CC=C1C=CC=N2
Synonyms

1,10-Phenanthroline
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light, stored under nitrogen

In solvent	-80°C	1 year
	-20°C	6 months

Publications (8)

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Mol Cell

The proteasome component PSMD14 drives myelomagenesis through a histone deubiquitinase activity. [Abstract]

2023 Nov 16;83(22):4000-4016.e6. PMID: 37935198

o-Phenanthroline purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]

The expression of RELA, SMAD3, MEN1, and PLK1 was detected by Western blot analysis in LP-1 or KMS11 cells lacking PSMD14 or NSD2, or in cells treated with the PSMD14 inhibitor o-Phenanthroline (OPA) or capsaicin.

o-Phenanthroline purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]

LP-1 or LP-1 BR cells were treated with vehicle,o-Phenanthroline (OPA), or capzimin for 24 h, followed by viability assessment.

o-Phenanthroline purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]

LP-1 or LP-1 BR cells were treated with vehicle, o-Phenanthroline (OPA), or capzimin, and were challenged with CPT for 24 h, followed by analysis for apoptosis.
Nat Commun

Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1. [Abstract]

2024 Jan 2;15(1):9. PMID: 38167274
Nat Commun

Reduced hepatic bradykinin degradation accounts for cold-induced BAT thermogenesis and WAT browning in male mice. [Abstract]

2023 May 2;14(1):2523. PMID: 37130842
Adv Sci (Weinh)

K29-Linked Ubiquitination of Transcription Regulators Controls Cell Proliferation in the Unfolded Protein Response. [Abstract]

2025 Aug 30:e09817. PMID: 40884253
Ind Crops Prod

2024 Sep 1, 215, 118652.
Organogenesis

TRABD2A promotes osteogenic differentiation of human periodontal ligament stem cells by modulating TNF-α and IL-1β. [Abstract]

2026 Dec 31;22(1):2673613. PMID: 42316890
STAR Protoc

Protocol for the generation of DDT signaling reporter cell line for CRISPR screening. [Abstract]

2026 Apr 24;7(2):104523. PMID: 42033729
bioRxiv

Comparative Genomic and Functional Profiling of ECM-Targeting Enzymes in Bacteroides, a Key Genus of the Human Gut Microbiome. [Abstract]

2026 Mar 5:2026.03.04.709643. PMID: 41847005

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (554.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (277.45 mM; Need ultrasonic)

H₂O : 2 mg/mL (11.10 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.5491 mL

27.7454 mL

55.4908 mL

5 mM

1.1098 mL

5.5491 mL

11.0982 mL

10 mM

0.5549 mL

2.7745 mL

5.5491 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (13.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (13.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (13.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (624 KB)
- English - EN (624 KB)
- Français - FR (624 KB)
- Deutsch - DE (624 KB)
- Norwegian - NO (624 KB)
- Español - ES (624 KB)
- Swedish - SV (624 KB)
- Italian - IT (624 KB)
- Korean - KR (624 KB)
- Portuguese - PT (624 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Guan NanMu, et al. Synergistic inhibition between o-phenanthroline and chloride ion on cold rolled steel corrosion in phosphoric acid. Materials Chemistry and Physics Volume 86, Issue 1, 15 July 2004, Pages 59-68.

[2]. Zi-Qiang Liu, et al. The role of matrix metalloprotease (MMP) to the autolysis of sea cucumber (Stichopus japonicus). J Sci Food Agric. 2019 Oct;99(13):5752-5759. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / Ethanol / DMSO	1 mM	5.5491 mL	27.7454 mL	55.4908 mL	138.7270 mL
	5 mM	1.1098 mL	5.5491 mL	11.0982 mL	27.7454 mL
	10 mM	0.5549 mL	2.7745 mL	5.5491 mL	13.8727 mL
Ethanol / DMSO	15 mM	0.3699 mL	1.8497 mL	3.6994 mL	9.2485 mL
	20 mM	0.2775 mL	1.3873 mL	2.7745 mL	6.9364 mL
	25 mM	0.2220 mL	1.1098 mL	2.2196 mL	5.5491 mL
	30 mM	0.1850 mL	0.9248 mL	1.8497 mL	4.6242 mL
	40 mM	0.1387 mL	0.6936 mL	1.3873 mL	3.4682 mL
	50 mM	0.1110 mL	0.5549 mL	1.1098 mL	2.7745 mL
	60 mM	0.0925 mL	0.4624 mL	0.9248 mL	2.3121 mL
	80 mM	0.0694 mL	0.3468 mL	0.6936 mL	1.7341 mL
	100 mM	0.0555 mL	0.2775 mL	0.5549 mL	1.3873 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

o-Phenanthroline

8 Cited Publications

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o-Phenanthroline

Publications Citing Use of MedChemExpress (MCE) o-Phenanthroline

All MMP Isoforms

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

o-Phenanthroline Related Classifications

HY-W004544 Related Classifications