o-Phenanthroline (Synonyms: 1,10-Phenanthroline)

Name: o-Phenanthroline
Brand: MedChemExpress
SKU: HY-W004544
Rating: 5.0 (5 reviews)

Cat. No.: HY-W004544 Purity: 99.80%: Data Sheet
COA

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o-Phenanthroline (1,10-Phenanthroline), a metal chelator, prevents the induction of chromosomal aberrations in streptozotocin-treated cells. o-Phenanthroline (1,10-Phenanthroline) forms a red chelate with Fe²⁺ that absorbs maximally at 510 nm. o-Phenanthroline (1,10-Phenanthroline) is also a MMP inhibitor.

For research use only. We do not sell to patients.

CAS No. : 66-71-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of o-Phenanthroline:

o-Phenanthroline monohydrate In-stock
o-Phenanthroline-d₈ Get quote

5 Publications Citing Use of MCE o-Phenanthroline

Cell Proliferation/Viability Assay

Flow Cytometry

: o-Phenanthroline purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]; The expression of RELA, SMAD3, MEN1, and PLK1 was detected by Western blot analysis in LP-1 or KMS11 cells lacking PSMD14 or NSD2, or in cells treated with the PSMD14 inhibitor o-Phenanthroline (OPA) or capsaicin.

: o-Phenanthroline purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]; LP-1 or LP-1 BR cells were treated with vehicle,o-Phenanthroline (OPA), or capzimin for 24 h, followed by viability assessment.

: o-Phenanthroline purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]; LP-1 or LP-1 BR cells were treated with vehicle, o-Phenanthroline (OPA), or capzimin, and were challenged with CPT for 24 h, followed by analysis for apoptosis.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	2.73 μM Compound: Phenanthroline	cytotoxic activity toward A2780 ovarian human cell line resistant (R) to cisplatin in experiment 3 cytotoxic activity toward A2780 ovarian human cell line resistant (R) to cisplatin in experiment 3	[PMID: 11000008]
A2780	IC₅₀	3.66 μM Compound: Phenanthroline	cytotoxic activity toward A2780 ovarian human cell line sensitive (S) to cisplatin in experiment 3 cytotoxic activity toward A2780 ovarian human cell line sensitive (S) to cisplatin in experiment 3	[PMID: 11000008]
COS-7	EC₅₀	3.9 μM Compound: Phe	Allosteric modulation at human CCR8 transfected in COS7 cells assessed as [3H]IP3 turnover by liquid scintillation counting analysis Allosteric modulation at human CCR8 transfected in COS7 cells assessed as [3H]IP3 turnover by liquid scintillation counting analysis	[PMID: 22957890]
COS-7	EC₅₀	4.9 μM Compound: Phe	Allosteric modulation at human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as [3H]IP3 turnover by liquid scintillation counting analysis Allosteric modulation at human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as [3H]IP3 turnover by liquid scintillation counting analysis	[PMID: 22957890]
COS-7	EC₅₀	5.9 μM Compound: Phe	Allosteric modulation at human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as {3H]IP3 turnover by liquid scintillation counting analysis Allosteric modulation at human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr assessed as {3H]IP3 turnover by liquid scintillation counting analysis	[PMID: 22957890]
COS-7	IC₅₀	53 μM Compound: Phe	Displacement of [125I]-CCL3 from human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs Displacement of [125I]-CCL3 from human CCR5 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs	[PMID: 22957890]
COS-7	IC₅₀	74 μM Compound: Phe	Displacement of [125I]-CCL3 from human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs Displacement of [125I]-CCL3 from human CCR1 transfected in COS7 cells coexpressing chimeric Gqi4myr after 3 hrs	[PMID: 22957890]
NHDF	CC₅₀	70.2 μM Compound: 1,10-Phen	Cytotoxicity against NHDF cells assessed as reduction in cell viability measured after 96 hrs by MTS assay Cytotoxicity against NHDF cells assessed as reduction in cell viability measured after 96 hrs by MTS assay	[PMID: 34666274]

In Vitro

o-Phenanthroline (1,10-Phenanthroline, 1mM) inhibits autolysis of sea cucumber body wall^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

180.21

Formula

C₁₂H₈N₂

CAS No.

66-71-7

Appearance

Solid

Color

White to off-white

SMILES

C12=C(N=CC=C3)C3=CC=C1C=CC=N2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light, stored under nitrogen

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (554.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (277.45 mM; Need ultrasonic)

H₂O : 2 mg/mL (11.10 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.5491 mL	27.7454 mL	55.4908 mL
5 mM	1.1098 mL	5.5491 mL	11.0982 mL
10 mM	0.5549 mL	2.7745 mL	5.5491 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (276 KB) SDS (624 KB)

COA (251 KB) HNMR (115 KB) RP-HPLC (260 KB)

Handling Instructions (2659 KB)

References

[1]. Guan NanMu, et al. Synergistic inhibition between o-phenanthroline and chloride ion on cold rolled steel corrosion in phosphoric acid. Materials Chemistry and Physics Volume 86, Issue 1, 15 July 2004, Pages 59-68.

[2]. Zi-Qiang Liu, et al. The role of matrix metalloprotease (MMP) to the autolysis of sea cucumber (Stichopus japonicus). J Sci Food Agric. 2019 Oct;99(13):5752-5759. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / Ethanol / DMSO	1 mM	5.5491 mL	27.7454 mL	55.4908 mL	138.7270 mL
	5 mM	1.1098 mL	5.5491 mL	11.0982 mL	27.7454 mL
	10 mM	0.5549 mL	2.7745 mL	5.5491 mL	13.8727 mL
Ethanol / DMSO	15 mM	0.3699 mL	1.8497 mL	3.6994 mL	9.2485 mL
	20 mM	0.2775 mL	1.3873 mL	2.7745 mL	6.9364 mL
	25 mM	0.2220 mL	1.1098 mL	2.2196 mL	5.5491 mL
	30 mM	0.1850 mL	0.9248 mL	1.8497 mL	4.6242 mL
	40 mM	0.1387 mL	0.6936 mL	1.3873 mL	3.4682 mL
	50 mM	0.1110 mL	0.5549 mL	1.1098 mL	2.7745 mL
	60 mM	0.0925 mL	0.4624 mL	0.9248 mL	2.3121 mL
	80 mM	0.0694 mL	0.3468 mL	0.6936 mL	1.7341 mL
	100 mM	0.0555 mL	0.2775 mL	0.5549 mL	1.3873 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

o-Phenanthroline Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

o-Phenanthroline66-71-71,10-PhenanthrolineEnvironmental PollutantsMMPEnvironmental ContaminantsMatrix metalloproteinasesInhibitorinhibitorinhibit

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o-Phenanthroline (Synonyms: 1,10-Phenanthroline)

Customer Review

Other Forms of o-Phenanthroline:

o-Phenanthroline Related Antibodies

5 Publications Citing Use of MCE o-Phenanthroline

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All MMP Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

o-Phenanthroline Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

o-Phenanthroline Related Classifications

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