o-Phenanthroline monohydrate
Based on 8 publication(s) in Google Scholar
o-Phenanthroline (1,10-Phenanthroline) monohydrate, a metal chelator, prevents the induction of chromosomal aberrations in streptozotocin-treated cells. o-Phenanthroline monohydrate forms a red chelate with Fe2+ that absorbs maximally at 510 nm. o-Phenanthroline (1,10-Phenanthroline) monohydrate is also a MMP inhibitor.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 5144-89-8
- Formula: C12H10N2O
- Molecular Weight:198.22
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) o-Phenanthroline monohydrate
More- Nat Commun. 2024 Jan 2;15(1):9. [Abstract]
- Nat Commun. 2023 May 2;14(1):2523. [Abstract]
- Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]
- Adv Sci (Weinh). 2025 Aug 30:e09817. [Abstract]
- Ind Crops Prod. 2024 Sep 1, 215, 118652.
- Organogenesis. 2026 Dec 31;22(1):2673613. [Abstract]
- STAR Protoc. 2026 Apr 24;7(2):104523. [Abstract]
- bioRxiv. 2026 Mar 5:2026.03.04.709643. [Abstract]
Customer Validation & Images
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WB
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Cell Proliferation/Viability Assay
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Flow Cytometry
Biological Activity
o-Phenanthroline (1,10-Phenanthroline, 1mM) inhibits autolysis of sea cucumber body wall[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 5144-89-8
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Appearance Solid
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Molecular Weight 198.22
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Formula C12H10N2O
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Color White to off-white
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SMILES
C12=C(N=CC=C3)C3=CC=C1C=CC=N2.O
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Synonyms
1,10-Phenanthroline monohydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (8)
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Journal Impact Factor
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Most Recent
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Nat Commun
Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1. [Abstract]2024 Jan 2;15(1):9. PMID: 38167274 -
Nat Commun
Reduced hepatic bradykinin degradation accounts for cold-induced BAT thermogenesis and WAT browning in male mice. [Abstract]2023 May 2;14(1):2523. PMID: 37130842 -
Mol Cell
The proteasome component PSMD14 drives myelomagenesis through a histone deubiquitinase activity. [Abstract]2023 Nov 16;83(22):4000-4016.e6. PMID: 37935198
o-Phenanthroline monohydrate purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]
The expression of RELA, SMAD3, MEN1, and PLK1 was detected by Western blot analysis in LP-1 or KMS11 cells lacking PSMD14 or NSD2, or in cells treated with the PSMD14 inhibitor o-Phenanthroline (OPA) or capsaicin.
o-Phenanthroline monohydrate purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]
LP-1 or LP-1 BR cells were treated with vehicle,o-Phenanthroline (OPA), or capzimin for 24 h, followed by viability assessment.
o-Phenanthroline monohydrate purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 16;83(22):4000-4016.e6. [Abstract]
LP-1 or LP-1 BR cells were treated with vehicle, o-Phenanthroline (OPA), or capzimin, and were challenged with CPT for 24 h, followed by analysis for apoptosis.
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Adv Sci (Weinh)
K29-Linked Ubiquitination of Transcription Regulators Controls Cell Proliferation in the Unfolded Protein Response. [Abstract]2025 Aug 30:e09817. PMID: 40884253 -
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Organogenesis
TRABD2A promotes osteogenic differentiation of human periodontal ligament stem cells by modulating TNF-α and IL-1β. [Abstract]2026 Dec 31;22(1):2673613. PMID: 42316890 -
STAR Protoc
2026 Apr 24;7(2):104523. PMID: 42033729 -
bioRxiv
Comparative Genomic and Functional Profiling of ECM-Targeting Enzymes in Bacteroides, a Key Genus of the Human Gut Microbiome. [Abstract]2026 Mar 5:2026.03.04.709643. PMID: 41847005
Solvent & Solubility
DMSO : 100 mg/mL (504.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2 mg/mL (10.09 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (624 KB)
- English - EN (624 KB)
- Français - FR (624 KB)
- Deutsch - DE (624 KB)
- Norwegian - NO (624 KB)
- Español - ES (624 KB)
- Swedish - SV (624 KB)
- Italian - IT (624 KB)
- Korean - KR (624 KB)
- Portuguese - PT (624 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.0449 mL | 25.2245 mL | 50.4490 mL | 126.1225 mL |
| 5 mM | 1.0090 mL | 5.0449 mL | 10.0898 mL | 25.2245 mL | |
| 10 mM | 0.5045 mL | 2.5224 mL | 5.0449 mL | 12.6122 mL | |
| DMSO | 15 mM | 0.3363 mL | 1.6816 mL | 3.3633 mL | 8.4082 mL |
| 20 mM | 0.2522 mL | 1.2612 mL | 2.5224 mL | 6.3061 mL | |
| 25 mM | 0.2018 mL | 1.0090 mL | 2.0180 mL | 5.0449 mL | |
| 30 mM | 0.1682 mL | 0.8408 mL | 1.6816 mL | 4.2041 mL | |
| 40 mM | 0.1261 mL | 0.6306 mL | 1.2612 mL | 3.1531 mL | |
| 50 mM | 0.1009 mL | 0.5045 mL | 1.0090 mL | 2.5224 mL | |
| 60 mM | 0.0841 mL | 0.4204 mL | 0.8408 mL | 2.1020 mL | |
| 80 mM | 0.0631 mL | 0.3153 mL | 0.6306 mL | 1.5765 mL | |
| 100 mM | 0.0504 mL | 0.2522 mL | 0.5045 mL | 1.2612 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
o-Phenanthroline monohydrate Related Classifications
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