Estramustine phosphate sodium

製品番号: HY-13627 純度: 99.42%: FAQs
Data Sheet SDS COA 取扱説明書

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Estramustine phosphate sodium, an estradiol analog, is an orally active antimicrotubule chemotherapy agent. Estramustine phosphate sodium depolymerises microtubules by binding to microtubule associated proteins (MAPs) and/or to tubulin. Estramustine phosphate sodium can interfere mitosis, trigger cell death and induce apoptosis, which can be used for the research of cancer like prostate cancer.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

Estramustine phosphate sodium 構造式

CAS 番号 : 52205-73-9

容量	価格（税別）	在庫状況	数量
Solid + Solvent
10 mM * 1 mL in Water ready for reconstitution	USD 56	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 56	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	USD 45	在庫あり	Estimated Time of Arrival: December 31
10 mg	USD 72	在庫あり	Estimated Time of Arrival: December 31
50 mg	USD 119	在庫あり	Estimated Time of Arrival: December 31
100 mg	USD 168	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

Other Forms of Estramustine phosphate sodium:

Estramustine phosphate お問い合わせ

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製品説明

体外実験

Estramustine phosphate sodium (1 µg/ mL, 48 h) suppresses PC3 cell growth^[1].
Estramustine phosphate sodium (2 µg/mL, 48 h) elevates phosphatidylserine eversion amount on PC3 cells and induces PC3 cell apoptosis through reducing miR-31^[1].
Estramustine phosphate sodium (0-40 µM, 24-72 h) inhibits cell proliferation and tubulin cytoskeleton in RAW 264.7 cells^[2].
Estramustine phosphate sodium (10 µM, 24 h) inhibits TGF-β-induced RAW 264.7 cell migration, as well as TGF-β-induced uPA production by inhibiting Smad3 activation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	PC3 cells
Concentration:	1 µg/ mL
Incubation Time:	48 h
Result:	Suppressed PC3 cell growth.

Immunofluorescence^[2]

Cell Line:	RAW 264.7 cells
Concentration:	10 µM
Incubation Time:	24 h
Result:	Disrupted the interphase microtubules.

Cell Migration Assay ^[2]

Cell Line:	18 h of TGF-β treated RAW 264.7 cells
Concentration:	10 µM
Incubation Time:	24 h
Result:	Inhibited TGF-β-Induced Cell Migration.

体内実験

Estramustine phosphate sodium (Intraperitoneal injection, 4 or 12 mg/kg, a daily dose for 2 weeks) inhibits PAC120 tumor growth 53% by day 35^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Swiss nu/nu (nude) male mice (5-week-old) bearing PAC120 tumors^[3]
Dosage:	4 mg/kg, 12 mg/kg
Administration:	Intraperitoneal injection; daily; for 2 weeks
Result:	Suppressed the development of skin lesions and resulted in a dissociation between DTH response and antibody production.

Animal Model:	Human prostate cancer xenograft PAC120^[3]
Dosage:	4 or 12 mg/kg, a daily dose for 2 weeks.
Administration:	Intraperitoneal injection
Result:	Inhibited PAC120 tumor growth 53% by day 35.

臨床実験

分子量

564.35

分子式

C₂₃H₃₀Cl₂NNa₂O₆P

CAS 番号

52205-73-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(N(CCCl)CCCl)OC1=CC2=C(C=C1)[C@@]3([H])CC[C@@]4(C)[C@](CC[C@@H]4OP(O[Na])(O[Na])=O)([H])[C@]3([H])CC2

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

H₂O : 62.5 mg/mL (110.75 mM; Need ultrasonic)

DMSO : 5 mg/mL (8.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7720 mL	8.8598 mL	17.7195 mL
5 mM	0.3544 mL	1.7720 mL	3.5439 mL
10 mM	0.1772 mL	0.8860 mL	1.7720 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 65 mg/mL (115.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.42%

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データシート (277 KB) SDS (252 KB)

COA (249 KB) LCMS (257 KB)

取扱説明書 (2659 KB)

参考文献

[1]. C Wei, et al. Estramustine phosphate induces prostate cancer cell line PC3 apoptosis by down-regulating miR-31 levels. Eur Rev Med Pharmacol Sci. 2018 Jan;22(1):40-45. [Content Brief]

[2]. Sonja S Mojsilovic, et al. Estramustine Phosphate Inhibits TGF- β-Induced Mouse Macrophage Migration and Urokinase-Type Plasminogen Activator Production. Anal Cell Pathol (Amst). 2018 Sep 2;2018:3134102. [Content Brief]

[3]. Stephane Oudard, et al. Activity of docetaxel with or without estramustine phosphate versus mitoxantrone in androgen dependent and independent human prostate cancer xenografts. J Urol. 2003 May;169(5):1729-34. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.7720 mL	8.8598 mL	17.7195 mL	44.2988 mL
DMSO / H₂O	5 mM	0.3544 mL	1.7720 mL	3.5439 mL	8.8598 mL
H₂O	10 mM	0.1772 mL	0.8860 mL	1.7720 mL	4.4299 mL
	15 mM	0.1181 mL	0.5907 mL	1.1813 mL	2.9533 mL
	20 mM	0.0886 mL	0.4430 mL	0.8860 mL	2.2149 mL
	25 mM	0.0709 mL	0.3544 mL	0.7088 mL	1.7720 mL
	30 mM	0.0591 mL	0.2953 mL	0.5907 mL	1.4766 mL
	40 mM	0.0443 mL	0.2215 mL	0.4430 mL	1.1075 mL
	50 mM	0.0354 mL	0.1772 mL	0.3544 mL	0.8860 mL
	60 mM	0.0295 mL	0.1477 mL	0.2953 mL	0.7383 mL
	80 mM	0.0221 mL	0.1107 mL	0.2215 mL	0.5537 mL
	100 mM	0.0177 mL	0.0886 mL	0.1772 mL	0.4430 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.