2. GPCR/G Protein Neuronal Signaling
  3. MCHR1 (GPR24)
  4. Melanin Concentrating Hormone, salmon

Melanin Concentrating Hormone, salmon  (Synonyms: MCH (salmon))

Cat. No.: HY-P1525 Purity: 98.40%
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Melanin Concentrating Hormone, salmon is a 19-amino-acid neuropeptide initially identified in the pituitary gland of teleost fish, which regulates food intake, energy balance, sleep state, and the cardiovascular system. Melanin-concentrating hormone is a ligand for an orphan G protein-coupled receptor (SLC-1/GPR24) and MCHR2.

For research use only. We do not sell to patients.

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Melanin Concentrating Hormone, salmon Chemical Structure

Melanin Concentrating Hormone, salmon Chemical Structure

CAS No. : 87218-84-6

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500 μg USD 140 In-stock
1 mg USD 220 In-stock
5 mg USD 880 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Melanin Concentrating Hormone, salmon:

Melanin Concentrating Hormone, salmon Related Antibodies

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  • Protocol

  • Purity & Documentation

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Description

Melanin Concentrating Hormone, salmon is a 19-amino-acid neuropeptide initially identified in the pituitary gland of teleost fish, which regulates food intake, energy balance, sleep state, and the cardiovascular system. Melanin-concentrating hormone is a ligand for an orphan G protein-coupled receptor (SLC-1/GPR24) and MCHR2.

IC50 & Target

SLC-1/GPR24, MCHR2[1]
In Vitro

An orphan G protein-coupled receptor (SLC-1/GPR24) has been identified as a receptor for MCH (MCHR1). MCHR2 has higher protein sequence homology to MCHR1 than any other G protein-coupled receptor. MCHR2 is specifically activated by nanomolar concentrations of MCH, binds to MCH with high affinity, and signals through Gq protein[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Melanin Concentrating Hormone, salmon Related Antibodies
In Vivo

Melanin Concentrating Hormone stimulates appetite. Continuous infusion of Melanin Concentrating Hormone into the ventricular system increases food intake for 7-8 days[2]. Intracerebroventricular infusion of Melanin Concentrating Hormone (10 μg/day) causes a slight but significant increase in body weight in mice maintained on the regular diet. Chronic stimulation of the brain Melanin Concentrating Hormone system could cause obesity in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

2099.48

Formula

C89H139N27O24S4
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val (Disulfide bridge: Cys5-Cys14)
Sequence Shortening

DTMRCMVGRVYRPCWEV (Disulfide bridge: Cys5-Cys14)
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (47.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.4763 mL 2.3815 mL 4.7631 mL
5 mM 0.0953 mL 0.4763 mL 0.9526 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C1

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Volume (start)

V1

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Kinase Assay
[1]

Binding of 125I-labeled Melanin Concentrating Hormone (MCH) to MCHR1 and MCHR2 is measured by filtration binding assay. Membranes (10 μg protein) from transiently transfected HEK293-MCHR1 and HEK293-MCHR2 cells are mixed with 0-9.8 nM 125I-labeled MCH in the binding buffer (50 mM Hepes/10 mM MgCl内容2/2 mM EGTA; protease inhibitors, 0.1% BSA, pH 7.6). After incubation for 1 h at room temperature, membrane-bound 125I-labeled MCH is separated from the free 125I-labeled MCH by filtration through a 96-well GF/B plate on a Packard Filtermate Cell Harvester and ished with ice-cold binding buffer supplemented with 80 mM NaCl. Eighty microliters of scintillation liquid is added, and the radioactivity is counted on a Packard Microplate Topcount[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2][3]

Rats[2]

Both male Wistar and male Sprague-Dawley rats weighing between 310 and 370 g (age 8-11 weeks) are used in the study. All animals receive food which had the following dry weight composition: 64% carbohydrate, 22% protein, 4.3% fat, 4% cellulose and 5.5% ash. For chronic experiments, the rats are infused either with artificial CSF (0.5 μL/h) or MCH (8 μg/rat/day).Body weight and food intake are then recorded daily for 12 days[2].

Mice[3]

Male C57BL/6J mice is prepared for measurement of spontaneous motor activity. Melanin Concentrating Hormone (10 μg/day) or the vehicle is infused for 14 days under the regular diet-fed condition. Motor activity is measured during the last 3 days of the 14-day infusion by an activity monitoring system in home cages[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.4763 mL 2.3815 mL 4.7631 mL 11.9077 mL
5 mM 0.0953 mL 0.4763 mL 0.9526 mL 2.3815 mL
10 mM 0.0476 mL 0.2382 mL 0.4763 mL 1.1908 mL
15 mM 0.0318 mL 0.1588 mL 0.3175 mL 0.7938 mL
20 mM 0.0238 mL 0.1191 mL 0.2382 mL 0.5954 mL
25 mM 0.0191 mL 0.0953 mL 0.1905 mL 0.4763 mL
30 mM 0.0159 mL 0.0794 mL 0.1588 mL 0.3969 mL
40 mM 0.0119 mL 0.0595 mL 0.1191 mL 0.2977 mL
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Melanin Concentrating Hormone, salmon Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Melanin Concentrating Hormone, salmon87218-84-6MCH (salmon)MCHR1 (GPR24)Melanin concentrating hormone receptor 1Inhibitorinhibitorinhibit

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