1. GPCR/G Protein
    Neuronal Signaling
  2. MCHR1 (GPR24)
  3. Melanin Concentrating Hormone, salmon

Melanin Concentrating Hormone, salmon (Synonyms: MCH (salmon))

Cat. No.: HY-P1525
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Melanin Concentrating Hormone, salmon is a 19-amino-acid neuropeptide initially identified in the pituitary gland of teleost fish, which regulates food intake, energy balance, sleep state, and the cardiovascular system. Melanin-concentrating hormone is a ligand for an orphan G protein-coupled receptor (SLC-1/GPR24) and MCHR2.

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Melanin Concentrating Hormone, salmon Chemical Structure

Melanin Concentrating Hormone, salmon Chemical Structure

CAS No. : 87218-84-6

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Description

Melanin Concentrating Hormone, salmon is a 19-amino-acid neuropeptide initially identified in the pituitary gland of teleost fish, which regulates food intake, energy balance, sleep state, and the cardiovascular system. Melanin-concentrating hormone is a ligand for an orphan G protein-coupled receptor (SLC-1/GPR24) and MCHR2.

IC50 & Target

SLC-1/GPR24, MCHR2[1]

In Vitro

An orphan G protein-coupled receptor (SLC-1/GPR24) has been identified as a receptor for MCH (MCHR1). MCHR2 has higher protein sequence homology to MCHR1 than any other G protein-coupled receptor. MCHR2 is specifically activated by nanomolar concentrations of MCH, binds to MCH with high affinity, and signals through Gq protein[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Melanin Concentrating Hormone stimulates appetite. Continuous infusion of Melanin Concentrating Hormone into the ventricular system increases food intake for 7-8 days[2]. Intracerebroventricular infusion of Melanin Concentrating Hormone (10 μg/day) causes a slight but significant increase in body weight in mice maintained on the regular diet. Chronic stimulation of the brain Melanin Concentrating Hormone system could cause obesity in mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

2099.48

Formula

C₈₉H₁₃₉N₂₇O₂₄S₄

CAS No.
Sequence

Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val (Disulfide bridge: Cys5-Cys14)

Sequence Shortening

DTMRCMVGRVYRPCWEV (Disulfide bridge: Cys5-Cys14)

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Kinase Assay
[1]

Binding of 125I-labeled Melanin Concentrating Hormone (MCH) to MCHR1 and MCHR2 is measured by filtration binding assay. Membranes (10 μg protein) from transiently transfected HEK293-MCHR1 and HEK293-MCHR2 cells are mixed with 0-9.8 nM 125I-labeled MCH in the binding buffer (50 mM Hepes/10 mM MgCl内容2/2 mM EGTA; protease inhibitors, 0.1% BSA, pH 7.6). After incubation for 1 h at room temperature, membrane-bound 125I-labeled MCH is separated from the free 125I-labeled MCH by filtration through a 96-well GF/B plate on a Packard Filtermate Cell Harvester and ished with ice-cold binding buffer supplemented with 80 mM NaCl. Eighty microliters of scintillation liquid is added, and the radioactivity is counted on a Packard Microplate Topcount[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2][3]

Rats[2]

Both male Wistar and male Sprague-Dawley rats weighing between 310 and 370 g (age 8-11 weeks) are used in the study. All animals receive food which had the following dry weight composition: 64% carbohydrate, 22% protein, 4.3% fat, 4% cellulose and 5.5% ash. For chronic experiments, the rats are infused either with artificial CSF (0.5 μL/h) or MCH (8 μg/rat/day).Body weight and food intake are then recorded daily for 12 days[2].

Mice[3]

Male C57BL/6J mice is prepared for measurement of spontaneous motor activity. Melanin Concentrating Hormone (10 μg/day) or the vehicle is infused for 14 days under the regular diet-fed condition. Motor activity is measured during the last 3 days of the 14-day infusion by an activity monitoring system in home cages[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

Melanin Concentrating Hormone, salmonMCH (salmon)MCHR1 (GPR24)Melanin concentrating hormone receptor 1Inhibitorinhibitorinhibit

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