Cat. No.: HY-17470

Mizoribine

(Synonyms: NSC 289637; HE 69)

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Mizoribine (NSC 289637), an imidazole nucleoside, inhibits HCV RNA replication with IC₅₀ of approximately 100 μM for anti-HCV activity. Immunosuppressant. Mizoribine, an IMPDH inhibitor, inhibits replication of SARS-CoV with IC₅₀s of 3.5 μg/mL and 16 μg/mL for SARS-CoV Frankfurt-1 and SARS-CoV HKU39849, respectively.

For research use only. We do not sell to patients.

Purity: 99.99%
CAS No.: 50924-49-7
Formula: C₉H₁₃N₃O₆
Molecular Weight:259.22

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Mizoribine Chemical Structure

CAS No.: 50924-49-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Mizoribine (NSC 289637), an imidazole nucleoside, inhibits HCV RNA replication with IC₅₀ of approximately 100 μM for anti-HCV activity. Immunosuppressant^[1]. Mizoribine, an IMPDH inhibitor, inhibits replication of SARS-CoV with IC₅₀s of 3.5 μg/mL and 16 μg/mL for SARS-CoV Frankfurt-1 and SARS-CoV HKU39849, respectively^[2].

IC₅₀ & Target

HCV^[1]; IMPDH; SARS-CoV^[2]

All Antibiotic Isoforms :

All Parasite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HT-1080	IC₅₀	>100 μg/mL Compound: bredinin	In vitro inhibitory activity against growth of Human fibrosarcoma (HT1080) cells In vitro inhibitory activity against growth of Human fibrosarcoma (HT1080) cells	[PMID: 1995901]
KB	IC₅₀	>100 μg/mL Compound: bredinin	In vitro inhibitory activity against growth of Human oral epidermoid carcinoma (KB) cells In vitro inhibitory activity against growth of Human oral epidermoid carcinoma (KB) cells	[PMID: 1995901]
Lu-65	IC₅₀	>100 μg/mL Compound: bredinin	In vitro inhibitory activity against growth of Human gaint cell carcinoma (Lu-65) cells In vitro inhibitory activity against growth of Human gaint cell carcinoma (Lu-65) cells	[PMID: 1995901]
OST	IC₅₀	>100 μg/mL Compound: bredinin	In vitro inhibitory activity against growth of Human osteosarcoma (OST) cells In vitro inhibitory activity against growth of Human osteosarcoma (OST) cells	[PMID: 1995901]
PC-13	IC₅₀	>100 μg/mL Compound: bredinin	In vitro inhibitory activity against growth of Human large-cell carcinoma (PC-13) cells In vitro inhibitory activity against growth of Human large-cell carcinoma (PC-13) cells	[PMID: 1995901]
PC-3	IC₅₀	>100 μg/mL Compound: bredinin	In vitro inhibitory activity against growth of Human lung adenocarcinoma (PC-3) cells. In vitro inhibitory activity against growth of Human lung adenocarcinoma (PC-3) cells.	[PMID: 1995901]
PC-6	IC₅₀	49 μg/mL Compound: bredinin	In vitro inhibitory activity against growth of Human lung small-cell carcinoma (PC-6) cells In vitro inhibitory activity against growth of Human lung small-cell carcinoma (PC-6) cells	[PMID: 1995901]
SW480	IC₅₀	42 μg/mL Compound: bredinin	In vitro inhibitory activity against growth of Human colon adenocarcinoma (SW-480) cells In vitro inhibitory activity against growth of Human colon adenocarcinoma (SW-480) cells	[PMID: 1995901]
T-24	IC₅₀	>100 μg/mL Compound: bredinin	In vitro inhibitory activity against growth of Human bladder transitional-cell carcinoma (T-24) cells In vitro inhibitory activity against growth of Human bladder transitional-cell carcinoma (T-24) cells	[PMID: 1995901]
Vero	CC₅₀	>700 μM Compound: mizoribin	Cytotoxicity against Vero E6 cells Cytotoxicity against Vero E6 cells	[PMID: 17336519]
Vero	IC₅₀	13.5 μM Compound: mizoribin	Antiviral activity against SARS-CoV Frankfurt1 in Vero E6 cells by plaque reduction assay Antiviral activity against SARS-CoV Frankfurt1 in Vero E6 cells by plaque reduction assay	[PMID: 17336519]

In Vitro

Mizoribine, isolated from culture medium of the mold Eupenicillium brefeldianum M-2166, acts as an immunosuppressant which exerts its effects without severe side effects^[1].
Mizoribine is an inosine-5′-monophosphate dehydrogenase (IMPDH) inhibitorused for renal transplantation, autoimmune diseases and steroid-resistant nephrotic syndrome ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Mizoribine suppresses collagen-induced arthritis. Mizoribine (10, 20 and 50 mg/kg; Administered orally 5 days a week for 12 weeks) reduces the arthritis score in a dose-dependent fashion, showing significant suppression, even at a dose of 10 mg/kg^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male DBA/1 J mice with collagen-induced arthritis^[3]
Dosage:

10, 20 and 50 mg/kg
Administration:

Administered orally 5 days a week for 12 weeks
Result:

Showed dose-dependency of the suppressive effect.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 50924-49-7
Appearance Solid
Molecular Weight 259.22
Formula C₉H₁₃N₃O₆
Color White to off-white
SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(O)=C(C(N)=O)N=C2)O1
Synonyms

NSC 289637; HE 69
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
- Microorganisms
Eupenicillium brefeldianum M-2166
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (5)

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Journal Impact Factor

Cell Death Differ

AF9 targets acetyl-modified STAT6 to diminish purine metabolism and accelerate cell apoptosis during metastasis. [Abstract]

2023 Jul;30(7):1695-1709. PMID: 37308587
Nucleic Acids Res

COVID19 Drug Repository: text-mining the literature in search of putative COVID19 therapeutics. [Abstract]

2021 Jan 8;49(D1):D1113-D1121. PMID: 33166390
mBio

Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C. [Abstract]

2022 Oct 26;13(5):e0143422. PMID: 36106732
Molecules

In Vitro and in Vivo Antiviral Activity of Mizoribine Against Foot-And-Mouth Disease Virus. [Abstract]

2019 May 3;24(9):1723. PMID: 31058822

Mizoribine purchased from MedChemExpress. Usage Cited in: Molecules. 2019 May 3;24(9):1723. [Abstract]

Guanosine supplementation attenuated the anti-FMDV effect of mizoribine. Serial dilutions of guanosine (from 100 to 25 μM) are added to FMDV (100 TCID₅₀ O/MY98/BY/2010)-infected IBRS-2 cells, when treated with 75 μM mizoribine for 48 h. (A) The cell viability of cells and (B) FMDV VP1 levels are analyzed.
bioRxiv

Systemic inhibition of de novo purine biosynthesis prevents weight gain and improves metabolic health by increasing thermogenesis and decreasing food intake. [Abstract]

2024 Nov 1:2024.10.28.620705. PMID: 39553975

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (385.78 mM)

DMSO : 62.5 mg/mL (241.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.8578 mL

19.2888 mL

38.5776 mL

5 mM

0.7716 mL

3.8578 mL

7.7155 mL

10 mM

0.3858 mL

1.9289 mL

3.8578 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (8.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (8.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (8.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: ≥ 100 mg/mL (385.78 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (421 KB)
- English - EN (421 KB)
- Français - FR (421 KB)
- Deutsch - DE (421 KB)
- Norwegian - NO (421 KB)
- Español - ES (421 KB)
- Swedish - SV (421 KB)
- Italian - IT (421 KB)
- Portuguese - PT (421 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Naka K, et al. Mizoribine inhibits hepatitis C virus RNA replication: effect of combination with interferon-alpha. Biochem Biophys Res Commun. 2005 May 13;330(3):871-9. [Content Brief]

[2]. Saijo M, et al. Inhibitory effect of Mizoribine and ribavirin on the replication of severe acute respiratory syndrome (SARS)-associated coronavirus. Antiviral Res. 2005 Jun;66(2-3):159-63. [Content Brief]

[3]. Kamada H, et al. Effect of Mizoribine on collagen-induced arthritis in mice. Jpn J Pharmacol. 1996 Feb;70(2):169-75. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	3.8578 mL	19.2888 mL	38.5776 mL	96.4439 mL
	5 mM	0.7716 mL	3.8578 mL	7.7155 mL	19.2888 mL
	10 mM	0.3858 mL	1.9289 mL	3.8578 mL	9.6444 mL
	15 mM	0.2572 mL	1.2859 mL	2.5718 mL	6.4296 mL
	20 mM	0.1929 mL	0.9644 mL	1.9289 mL	4.8222 mL
	25 mM	0.1543 mL	0.7716 mL	1.5431 mL	3.8578 mL
	30 mM	0.1286 mL	0.6430 mL	1.2859 mL	3.2148 mL
	40 mM	0.0964 mL	0.4822 mL	0.9644 mL	2.4111 mL
	50 mM	0.0772 mL	0.3858 mL	0.7716 mL	1.9289 mL
	60 mM	0.0643 mL	0.3215 mL	0.6430 mL	1.6074 mL
	80 mM	0.0482 mL	0.2411 mL	0.4822 mL	1.2055 mL
	100 mM	0.0386 mL	0.1929 mL	0.3858 mL	0.9644 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mizoribine

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Publications Citing Use of MedChemExpress (MCE) Mizoribine

All Antibiotic Isoforms

All Parasite Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Mizoribine Related Classifications

Cat. No.: HY-17470