1. Cell Cycle/DNA Damage
    Apoptosis
  2. CDK
    Apoptosis
  3. NVP-2

NVP-2 

Cat. No.: HY-12214A Purity: 99.29%
Handling Instructions

NVP-2 is a potent and selective ATP-competitive cyclin dependent kinase 9 (CDK9) probe, inhibits CDK9/CycT activity with an IC50 of 0.514 nM. NVP-2 displays inhibitory effcts on CDK1/CycB, CDK2/CycA and CDK16/CycY kinases with IC50 values of 0.584 µM, 0.706 µM, and 0.605 µM, respectively. NVP-2 induces cell apoptosis.

For research use only. We do not sell to patients.

NVP-2 Chemical Structure

NVP-2 Chemical Structure

CAS No. : 1263373-43-8

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 282 In-stock
Estimated Time of Arrival: December 31
1 mg USD 180 In-stock
Estimated Time of Arrival: December 31
5 mg USD 250 In-stock
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10 mg USD 400 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1400 In-stock
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100 mg USD 2500 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 10 publication(s) in Google Scholar

Top Publications Citing Use of Products

    NVP-2 purchased from MCE. Usage Cited in: Cell. 2018 Sep 20;175(1):171-185.e25.

    WB analysis of MV4-11 cells treated with BTX161 (6 hr), iCDK9 (4 hr), or THZ1 (4 hr) at the indicated concentrations in different combinations as indicated. PP2Ac is a loading control.

    NVP-2 purchased from MCE. Usage Cited in: Neoplasia. 2019 Jul;21(7):713-720.

    LNCaP cells were treated with DMSO or increasing dose of NVP2 for 24 hours and samples are analyzed using western blotting. Densitometry is used to evaluate the abundance of each protein.
    • Biological Activity

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    • References

    • Customer Review

    Description

    NVP-2 is a potent and selective ATP-competitive cyclin dependent kinase 9 (CDK9) probe, inhibits CDK9/CycT activity with an IC50 of 0.514 nM. NVP-2 displays inhibitory effcts on CDK1/CycB, CDK2/CycA and CDK16/CycY kinases with IC50 values of 0.584 µM, 0.706 µM, and 0.605 µM, respectively. NVP-2 induces cell apoptosis.[1]

    IC50 & Target

    CDK9

    0.5 nM (IC50)

    In Vitro

    NVP-2 has an anti-proliferation of leukemia cells, inhibits KOPT-K1, Jurkat, P12-ICHIKAWA, DU.528, MOLT 16, HSB-2, PF-382, SKW-3, SUP-T11, DND-41 and HPB-ALL cells with IC50 values of 0.1688 µM, 0.1233 µM,0.5736 µM,0.1575 µM, 0.1620 µM,0.1585 µM, 0.1808 µM, 0.2589 µM, 0.0918 µM and 0.3023 µM, respectively[1].
    NVP-2 (250 nM-1 μM; 6 hours) engages CDK9 in wildtype and CRBN−/− MOLT4 cells at all concentrations, while CDK2 and CDK7 are unaffected[1].
    NVP-2 (0-10 nM; 72 hours) exhibits CRBN-dependent anti-proliferative and pro-apoptotic effects in MOLT4 cells, displays an IC50 value of 9 nM[1].
    NVP-2 (250 nM; 24 hours) induces cell apoptosis in MOLT4 cells, upregulates caspase-3 and γH2A.X expression. However, while the compound washout significantly reduces the degree of apoptosis induced by NVP-2[1].

    Cell Viability Assay[1]

    Cell Line: Leukemia cell lines
    Concentration: 0.1233 µM-0.5736 µM
    Incubation Time: 72 hours
    Result: Inhibited Leukemia cell lines viabity.

    Western Blot Analysis[1]

    Cell Line: Wildtype and CRBN−/− MOLT4 cells
    Concentration: 1 μM; 500 nM; 250 nM
    Incubation Time: 6 hours
    Result: Degrade CDK9  sub-stoichiometrically at all concentrations.

    Apoptosis Analysis[1]

    Cell Line: Wildtype and CRBN−/− MOLT4 cells
    Concentration: 250 nM
    Incubation Time: 24 hours
    Result: Induced cell apoptosis in cells and wash outed the compound relieved NVP-2-induced cell apoptosis.

    Cell Proliferation Assay[1]

    Cell Line: MOLT4 cells
    Concentration: 0-10 nM
    Incubation Time: 72 hours
    Result: Exhibited anti-proliferative effects in MOLT4 cells.
    Molecular Weight

    513.07

    Formula

    C₂₇H₃₇ClN₆O₂

    CAS No.

    1263373-43-8

    SMILES

    ClC1=CN=C(N[[email protected]]2CC[[email protected]](N[[email protected]](C)COC)CC2)C=C1C3=CC=CC(NCC4(C#N)CCOCC4)=N3

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (194.91 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9491 mL 9.7453 mL 19.4905 mL
    5 mM 0.3898 mL 1.9491 mL 3.8981 mL
    10 mM 0.1949 mL 0.9745 mL 1.9491 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (4.87 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (4.87 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.87 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.29%

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    Keywords:

    NVP-2NVP2NVP 2CDKApoptosisCyclin dependent kinaseCRBNCDK9LeukemiaInhibitorinhibitorinhibit

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    Product name:
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    Cat. No.:
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