2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-112641A
    AkaLumine hydrochloride
    		99.92%
    AkaLumine hydrochloride is a D-luciferin (HY-12591A) analogue with a Km of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine hydrochloride emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine hydrochloride has high tissue-penetration and increases detection sensitivity from deep-tissue targets.
    AkaLumine hydrochloride
  • HY-D0822
    CY3
    		99.87%
    Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
    CY3
  • HY-101899
    Monochlorobimane
    		99.86%
    Monochlorobimane (Chlorobimane) is a fluorescent dye (λex=380 nm, λem=470 nm) to measure glutathione (GSH) in cellular assays.
    Monochlorobimane
  • HY-D0090
    MQAE
    		99.42%
    MQAE is a chloride ion (Cl-) fluorescent probe that can be used to measure chloride concentrations. The fluorescence intensity of MQAE decreases proportionally as Cl- ions increase. MQAE has high cell permeability and is suitable for fluorescence detection such as confocal microscopy and flow cytometry (Ex/Em=350/460 nm).
    MQAE
  • HY-15934
    X-GAL
    		99.88%
    X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity.
    X-GAL
  • HY-15142R
    Doxorubicin hydrochloride (Standard)
    Doxorubicin hydrochloride (Standard) is the analytical standard of Doxorubicin hydrochloride. This product is intended for research and analytical applications. Doxorubicin (Hydroxydaunorubicin) hydrochloride, a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent. Doxorubicin hydrochloride is a potent human DNA topoisomerase I and topoisomerase II inhibitor with IC50s of 0.8 μM and 2.67 μM, respectively. Doxorubicin hydrochloride reduces basal phosphorylation of AMPK and its downstream target acetyl-CoA carboxylase. Doxorubicin hydrochloride induces apoptosis and autophagy.
    Doxorubicin hydrochloride (Standard)
  • HY-D0040
    Calcein
    Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems.
    Calcein
  • HY-B0324A
    Crystal Violet
    Crystal Violet, also known as Gentian violet, methyl violet 10B, is a triphenyl-methane, an alkaline dye that binds to DNA in the nucleus of a cell, staining it a deep purple. It is often used for Gram staining to classify bacteria, or for cell or histological staining[1].
    Crystal Violet
  • HY-Y0016
    Rhodamine B
    		98.0%
    Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.
    Rhodamine B
  • HY-W019823
    4-MUNANA
    		98.08%
    4-MUNANA is a substrate of influenza virus neuraminidase (NA) with high selectivity and irreversible reaction. In the enzymatic reaction, 4-MUNANA is hydrolyzed by NA to generate fluorescent 4-methylumbelliferone (4-MU). By detecting the fluorescence intensity of 4-MU, quantitative analysis of NA activity can be achieved. 4-MUNANA can be used in influenza-related research, such as screening NA inhibitors, developing new anti-influenza drugs, and studying the infection mechanism of influenza viruses.
    4-MUNANA
  • HY-D1421
    PKH 67
    		≥99.0%
    PKH67 is a fluorescent cell binding dye with green fluorescence. PKH67 can stain the cell membrane and the Ex/Em is 490/502 nm. PKH67 is often used in combination with the non-specific red fluorescent dye PKH26 (Ex/Em=551/567 nm) to label cells, detect cell proliferation in vitro, and trace cells in vitro and in vivo.
    PKH 67
  • HY-15902
    ABTS diammonium salt
    		99.99%
    ABTS diammonium salt (AzBTS-(NH4)2) is a substrate for horseradish peroxidase (HRP) conjugate. ABTS diammonium salt can be used to evaluate antioxidant capacity.
    ABTS diammonium salt
  • HY-W011664
    1,3-Diphenylisobenzofuran
    		98.90%
    1,3-Diphenylisobenzofuran (DPBF) has been developed as a selective probe for the detection and quantitative determination of hydrogen peroxide in samples containing different reactive nitrogen and oxygen species (RNOS). DPBF is a fluorescent probe which, for almost 20 years, was believed to react in a highly specific manner toward some reactive oxygen species such as singlet oxygen and hydroxy, alkyloxy or alkylperoxy radicals.
    1,3-Diphenylisobenzofuran
  • HY-111330
    Hydroxyphenyl Fluorescein
    		99.91%
    Hydroxyphenyl Fluorescein (HPF) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940). Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm.
    Hydroxyphenyl Fluorescein
  • HY-U00451
    ATP-Red 1
    		≥98.0%
    ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).
    ATP-Red 1
  • HY-138226
    BODIPY 558/568 C12
    		98.42%
    BODIPY 558/568 C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 558/568 nm.
    BODIPY 558/568 C12
  • HY-126561
    Green CMFDA
    		99.82%
    Green CMFDA is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.
    Green CMFDA
  • HY-15925
    Nitro blue tetrazolium chloride
    		99.82%
    Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
    Nitro blue tetrazolium chloride
  • HY-D2908
    SYTO9
    		99.08%
    SYTO9 is a green fluorescent nucleic acid dye (Ex = 485 nm, Em = 498 nm (DNA)/501 nm (RNA)). SYTO9 can penetrate the intact cell membrane of viable bacteria. When the cell membrane is damaged (cell death), its rate of entry into cells increases, and it competes with propidium iodide (PI) (HY-D0815), another membrane-impermeable dye, for nucleic acid binding sites. SYTO9 is widely used in fluorescence microscopy, flow cytometry, and fluorometric assays in microbiology for bacterial counting, viability assessment, and cell imaging.
    SYTO9
  • HY-128868G
    FITC-Dextran (MW 150000)
    FITC-Dextran (MW 150000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=491 nm; Em=518 nm). FITC-Dextran (MW 150000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 150000) can be used in perfusion studies in animals or in fluorescence microlymphography, to study processes that affect the permeability of the blood brain barrier (BBB). FITC-Dextran (MW 150000) can be used as fluorescent probe to study cell permeability.
    FITC-Dextran (MW 150000)
Cat. No. Product Name / Synonyms Application Reactivity