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  4. Green CMFDA

Green CMFDA is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.

For research use only. We do not sell to patients.

Green CMFDA Chemical Structure

Green CMFDA Chemical Structure

CAS No. : 136832-63-8

Size Price Stock Quantity
1 mg USD 150 In-stock
5 mg USD 525 In-stock
10 mg USD 900 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Green CMFDA Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Green CMFDA is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope[1].

In Vitro

Allow the product to warm to room temperature before breaking seal. Dissolve Green CMFDA in high-quality DMSO to a final concentration of 10 mM as stock solution. Dilute the stock solution to a final working concentration of 0.5-5 µM for short term staining and 5-25 µM for long term staining in serumfree medium. Warm the working solution to 37 ℃.
For cells in suspension:
1. Harvest cells by centrifugation and aspirate the supernatant. Resuspend the cells gently in pre-warmed working solution.
2. Incubate 15–45 min under growth conditions.
3. Centrifuge the cells and remove the working solution.
4. Add culture media and dispense the labeled cell onto slide or into a culture vessel of choice.

For adherent cells:
1. Remove culture media.
2. Gently add the pre-warmed working solution.
3. Incubate 15–45 min under growth conditions.
4. Remove the working solution.
5. Add culture media.

Observe with Emission light of 514 nm and Excitation light of 485 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Green CMFDA Related Antibodies
Molecular Weight

464.85

Formula

C25H17ClO7
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

514
Excitation (Ex)

485
SMILES

O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=C(CCl)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (71.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1512 mL 10.7562 mL 21.5123 mL
5 mM 0.4302 mL 2.1512 mL 4.3025 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.43 mg/mL (3.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.43 mg/mL (3.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.71%

COA (254 KB) HNMR (270 KB) LCMS (269 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1512 mL 10.7562 mL 21.5123 mL 53.7808 mL
5 mM 0.4302 mL 2.1512 mL 4.3025 mL 10.7562 mL
10 mM 0.2151 mL 1.0756 mL 2.1512 mL 5.3781 mL
15 mM 0.1434 mL 0.7171 mL 1.4342 mL 3.5854 mL
20 mM 0.1076 mL 0.5378 mL 1.0756 mL 2.6890 mL
25 mM 0.0860 mL 0.4302 mL 0.8605 mL 2.1512 mL
30 mM 0.0717 mL 0.3585 mL 0.7171 mL 1.7927 mL
40 mM 0.0538 mL 0.2689 mL 0.5378 mL 1.3445 mL
50 mM 0.0430 mL 0.2151 mL 0.4302 mL 1.0756 mL
60 mM 0.0359 mL 0.1793 mL 0.3585 mL 0.8963 mL
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Green CMFDA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Green CMFDA136832-63-8CMFDAFluorescent Dyecell tracerfluorescent probenon-specific esterasesInhibitorinhibitorinhibit

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