Green CMFDA
Based on 12 publication(s) in Google Scholar
Green CMFDA is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.
For research use only. We do not sell to patients.
- Purity: 99.82%
- CAS No.: 136832-63-8
- Formula: C25H17ClO7
- Molecular Weight:464.85
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Green CMFDA
More- Adv Mater. 2025 Aug 29:e02800. [Abstract]
- Adv Sci (Weinh). 2025 Nov 9:e15349. [Abstract]
- Mater Today Bio. 2024 Aug 24:28:101212. [Abstract]
- J Neuroinflammation. 2025 Mar 19;22(1):88. [Abstract]
- Cell Death Dis. 2025 Jan 14;16(1):17. [Abstract]
- Pharmaceuticals (Basel). 2026 Mar 18;19(3):499. [Abstract]
- Eur J Pharmacol. 2025 Dec 4:1011:178432. [Abstract]
- Int Immunopharmacol. 2025 Oct 15:167:115599. [Abstract]
- ACS Infect Dis. 2025 Jun 13;11(6):1563-1576. [Abstract]
- Hematology. 2025 Dec;30(1):2545078. [Abstract]
- Res Sq. 2026 May 6.
- SSRN. 2026 Mar 2.
Customer Validation & Images
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IF
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Flow Cytometry
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Allow the product to warm to room temperature before breaking seal. Dissolve Green CMFDA in high-quality DMSO to a final concentration of 10 mM as stock solution. Dilute the stock solution to a final working concentration of 0.5-5 μM for short term staining and 5-25 μM for long term staining in serumfree medium. Warm the working solution to 37 °C.
For cells in suspension:
1. Harvest cells by centrifugation and aspirate the supernatant. Resuspend the cells gently in pre-warmed working solution.
2. Incubate 15–45 min under growth conditions.
3. Centrifuge the cells and remove the working solution.
4. Add culture media and dispense the labeled cell onto slide or into a culture vessel of choice.
For adherent cells:
1. Remove culture media.
2. Gently add the pre-warmed working solution.
3. Incubate 15–45 min under growth conditions.
4. Remove the working solution.
5. Add culture media.
6. Observe with Emission light of 514 nm and Excitation light of 485 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
514
485
Chemical Information
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CAS No. 136832-63-8
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Appearance Solid
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Molecular Weight 464.85
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Formula C25H17ClO7
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Color White to off-white
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=C(CCl)C=C5
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Synonyms
CMFDA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (12)
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Journal Impact Factor
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Most Recent
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Adv Mater
An Epigenetic Nanoagonist Facilitates T Cell Priming, Recruitment, and Reinvigoration in Tumors Resistant to PD-L1 Therapy. [Abstract]2025 Aug 29:e02800. PMID: 40883972 -
Adv Sci (Weinh)
Photo-Empowered Macrophage-Based Drug Delivery System Overcomes Motility Suppression and Significantly Enhances Deep Tumor Drug Delivery. [Abstract]2025 Nov 9:e15349. PMID: 41208150 -
Mater Today Bio
Tough and self-adhesive zwitterionic hydrogels with mechano-responsive release of bFGF for tympanic membrane repair. [Abstract]2024 Aug 24:28:101212. PMID: 39687063 -
J Neuroinflammation
SIRPα modulates microglial efferocytosis and neuroinflammation following experimental subarachnoid hemorrhage via the SHP1/STAT6 axis. [Abstract]2025 Mar 19;22(1):88. PMID: 40108663
Green CMFDA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Mar 19;22(1):88. [Abstract]
Representative immunofluorescence images of Green CMFDA (30 min) (green) and Iba1 (red) in microglia after 10µM Hb and ACs stimulation for 12 h. Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm.
Green CMFDA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Mar 19;22(1):88. [Abstract]
Flow cytometry analysis and quantification of Green CMFDA (30 min)+Iba1+ microglia (n = 3 each group).
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Cell Death Dis
2025 Jan 14;16(1):17. PMID: 39809787
Green CMFDA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Jan 14;16(1):17. [Abstract]
A high-resolution live-cell imaging was performed to follow up the activity of Jurkat T cells co-cultured with AGS cells overexpressing CagA and SQLE; living tumor cells: red (DiD perchlorate); living Jurkat T cells: green (Green CMFDA). Scale bar = 100 μm.
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Pharmaceuticals (Basel)
Myricetin Inhibits Osteosarcoma Cell Viability and Modulates EMT-Related Genes Associated with the SNAI1/MMP-9 Axis. [Abstract]2026 Mar 18;19(3):499. PMID: 41901344 -
Eur J Pharmacol
Robinetin inhibits osteosarcoma proliferation and migration, acting via the Slug/Twist signaling axis. [Abstract]2025 Dec 4:1011:178432. PMID: 41352702
Green CMFDA purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2025 Dec 4:1011:178432. [Abstract]
Representative images of 143B cell invasion at 0 and 24 h, following exposure to robinetin (A2) or robinetin/doxorubicin (Dox) combinations. MRC-5 lung cells were stained with Cell TrackerTM Red CMTPX dye and 143B osteosarcoma cells with Cell TrackerTM Green CMFDA (10 μM; 15 min) dye.
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Int Immunopharmacol
Deficiency of the innate immune protein IFITM3 impairs phagocytosis and promotes autism-like behaviors in mice. [Abstract]2025 Oct 15:167:115599. PMID: 41101227 -
ACS Infect Dis
An Antibody-Recruiting Molecule Enhances Fcγ Receptor-Mediated Uptake and Killing of Mycobacterial Pathogens by Macrophages. [Abstract]2025 Jun 13;11(6):1563-1576. PMID: 40312277
Green CMFDA purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2025 Jun 13;11(6):1563-1576. [Abstract]
Tre-DNP increases phagolysosome fusion. Mabs was either labeled with Tre-DNP or left unlabeled and stained with 20 μM Green CMFDA (1 h) (green). Bacteria were then incubated in buffered saline or opsonized with anti-DNP antibody. The bacteria (2 × 106 CFU/mL) were cocultured with J774 murine macrophages (4 × 105 cells/mL) for 1 h, and extracellular bacteria were eliminated with 100 μg/mL gentamicin. At each time point, cells were stained with 0.5 μM LysoTracker Deep Red (red) and imaged by fluorescence microscopy. Shown are two representative merged images of macrophages 4 h post infection. Yellow depicts overlap between Green CMFDA and LysoTracker Deep Red (B, scale bar = 100 μM)
Green CMFDA purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2025 Jun 13;11(6):1563-1576. [Abstract]
CMFDA stains mycobacterial pathogens without affecting Tre-DNP labeling. Mabs was labeled with Tre-DNP (250 μM) (or left unlabeled), then washed and stained with Green CMFDA (10 μM; 1 h) (or left unstained). Bacteria were washed, treated with mouse anti-DNP antibody (or left untreated), washed, and stained with PE-conjugated rabbit antimouse IgG antibody. Cells were fixed, washed, and analyzed by flow cytometry. Percentage of the population positive for fluorescein (CMFDA+), representing CMFDA-stained cells.
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Hematology
Brutons tyrosine kinase inhibitor Zanubrutinib modulates Fc gamma receptors to inhibit platelet destruction for alleviation of refractory immune thrombocytopenia. [Abstract]2025 Dec;30(1):2545078. PMID: 41047944 -
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Solvent & Solubility
DMSO : 33.33 mg/mL (71.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (614 KB)
- English - EN (614 KB)
- Français - FR (614 KB)
- Deutsch - DE (614 KB)
- Norwegian - NO (614 KB)
- Español - ES (614 KB)
- Swedish - SV (614 KB)
- Italian - IT (614 KB)
- Portuguese - PT (614 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.1512 mL | 10.7562 mL | 21.5123 mL | 53.7808 mL |
| 5 mM | 0.4302 mL | 2.1512 mL | 4.3025 mL | 10.7562 mL | |
| 10 mM | 0.2151 mL | 1.0756 mL | 2.1512 mL | 5.3781 mL | |
| 15 mM | 0.1434 mL | 0.7171 mL | 1.4342 mL | 3.5854 mL | |
| 20 mM | 0.1076 mL | 0.5378 mL | 1.0756 mL | 2.6890 mL | |
| 25 mM | 0.0860 mL | 0.4302 mL | 0.8605 mL | 2.1512 mL | |
| 30 mM | 0.0717 mL | 0.3585 mL | 0.7171 mL | 1.7927 mL | |
| 40 mM | 0.0538 mL | 0.2689 mL | 0.5378 mL | 1.3445 mL | |
| 50 mM | 0.0430 mL | 0.2151 mL | 0.4302 mL | 1.0756 mL | |
| 60 mM | 0.0359 mL | 0.1793 mL | 0.3585 mL | 0.8963 mL |
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