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  4. Green CMFDA

Green CMFDA is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.

For research use only. We do not sell to patients.

CAS No. : 136832-63-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Green CMFDA:

Green CMFDA Related Antibodies

Top Publications Citing Use of Products

    Green CMFDA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Mar 19;22(1):88.  [Abstract]

    Representative immunofluorescence images of Green CMFDA (30 min) (green) and Iba1 (red) in microglia after 10µM Hb and ACs stimulation for 12 h. Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm.

    Green CMFDA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Mar 19;22(1):88.  [Abstract]

    Flow cytometry analysis and quantification of Green CMFDA (30 min)+Iba1+ microglia (n = 3 each group).

    Green CMFDA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Jan 14;16(1):17.  [Abstract]

    A high-resolution live-cell imaging was performed to follow up the activity of Jurkat T cells co-cultured with AGS cells overexpressing CagA and SQLE; living tumor cells: red (DiD perchlorate); living Jurkat T cells: green (Green CMFDA). Scale bar = 100 μm.

    Green CMFDA purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2025 Dec 4:1011:178432.  [Abstract]

    Representative images of 143B cell invasion at 0 and 24 h, following exposure to robinetin (A2) or robinetin/doxorubicin (Dox) combinations. MRC-5 lung cells were stained with Cell TrackerTM Red CMTPX dye and 143B osteosarcoma cells with Cell TrackerTM Green CMFDA (10 μM; 15 min) dye.

    Green CMFDA purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2025 Jun 13;11(6):1563-1576.  [Abstract]

    Tre-DNP increases phagolysosome fusion. Mabs was either labeled with Tre-DNP or left unlabeled and stained with 20 μM Green CMFDA (1 h) (green). Bacteria were then incubated in buffered saline or opsonized with anti-DNP antibody. The bacteria (2 × 106 CFU/mL) were cocultured with J774 murine macrophages (4 × 105 cells/mL) for 1 h, and extracellular bacteria were eliminated with 100 μg/mL gentamicin. At each time point, cells were stained with 0.5 μM LysoTracker Deep Red (red) and imaged by fluorescence microscopy. Shown are two representative merged images of macrophages 4 h post infection. Yellow depicts overlap between Green CMFDA and LysoTracker Deep Red (B, scale bar = 100 μM)

    Green CMFDA purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2025 Jun 13;11(6):1563-1576.  [Abstract]

    CMFDA stains mycobacterial pathogens without affecting Tre-DNP labeling. Mabs was labeled with Tre-DNP (250 μM) (or left unlabeled), then washed and stained with Green CMFDA (10 μM; 1 h) (or left unstained). Bacteria were washed, treated with mouse anti-DNP antibody (or left untreated), washed, and stained with PE-conjugated rabbit antimouse IgG antibody. Cells were fixed, washed, and analyzed by flow cytometry. Percentage of the population positive for fluorescein (CMFDA+), representing CMFDA-stained cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Green CMFDA is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope[1].

    In Vitro


    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    Allow the product to warm to room temperature before breaking seal. Dissolve Green CMFDA in high-quality DMSO to a final concentration of 10 mM as stock solution. Dilute the stock solution to a final working concentration of 0.5-5 μM for short term staining and 5-25 μM for long term staining in serumfree medium. Warm the working solution to 37 °C.
    For cells in suspension:
    1. Harvest cells by centrifugation and aspirate the supernatant. Resuspend the cells gently in pre-warmed working solution.
    2. Incubate 15–45 min under growth conditions.
    3. Centrifuge the cells and remove the working solution.
    4. Add culture media and dispense the labeled cell onto slide or into a culture vessel of choice.

    For adherent cells:
    1. Remove culture media.
    2. Gently add the pre-warmed working solution.
    3. Incubate 15–45 min under growth conditions.
    4. Remove the working solution.
    5. Add culture media.
    6. Observe with Emission light of 514 nm and Excitation light of 485 nm.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Green CMFDA Related Antibodies
    Molecular Weight

    464.85

    Formula

    C25H17ClO7
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Emission (Em)

    514
    Excitation (Ex)

    485
    SMILES

    O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=C(CCl)C=C5
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (71.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1512 mL 10.7562 mL 21.5123 mL
    5 mM 0.4302 mL 2.1512 mL 4.3025 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.82%

    SDS (614 KB)

    COA (254 KB) HNMR (252 KB) LCMS (112 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1512 mL 10.7562 mL 21.5123 mL 53.7808 mL
    5 mM 0.4302 mL 2.1512 mL 4.3025 mL 10.7562 mL
    10 mM 0.2151 mL 1.0756 mL 2.1512 mL 5.3781 mL
    15 mM 0.1434 mL 0.7171 mL 1.4342 mL 3.5854 mL
    20 mM 0.1076 mL 0.5378 mL 1.0756 mL 2.6890 mL
    25 mM 0.0860 mL 0.4302 mL 0.8605 mL 2.1512 mL
    30 mM 0.0717 mL 0.3585 mL 0.7171 mL 1.7927 mL
    40 mM 0.0538 mL 0.2689 mL 0.5378 mL 1.3445 mL
    50 mM 0.0430 mL 0.2151 mL 0.4302 mL 1.0756 mL
    60 mM 0.0359 mL 0.1793 mL 0.3585 mL 0.8963 mL
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    Green CMFDA Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Green CMFDA136832-63-8CMFDAFluorescent Dyecell tracerfluorescent probenon-specific esterasesInhibitorinhibitorinhibit

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