N-Phenylnaphthalen-1-amine

Name: N-Phenylnaphthalen-1-amine
Brand: MedChemExpress
SKU: HY-W009756
Rating: 5.0 (3 reviews)

Cat. No.: HY-W009756 Purity: 99.98%: Data Sheet
COA

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Handling Instructions
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N-Phenyl-1-naphthylamine is a dye that fluoresces strongly when bound to the inner phospholipid bilayer of Gram-negative bacteria. N-Phenyl-1-naphthylamine can be used to measure outer membrane permeability. N-Phenylnaphthalen-1-amine is a fluorescence probe for odorant-binding proteins (OBP) with a dissociation constant of 1.67 μM. N-Phenylnaphthalen-1-amine exhibits an excitation wavelength of 337 nM and an emission wavelength of 407 nM.

For research use only. We do not sell to patients.

CAS No. : 90-30-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
500 g	In-stock	Estimated Time of Arrival: December 31
1 kg	In-stock	Estimated Time of Arrival: December 31

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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE N-Phenylnaphthalen-1-amine

Others

: N-Phenylnaphthalen-1-amine purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2025 Oct:120:105923. [Abstract]; Determination of outer membrane integrity. The outer membrane permeability of mcr-1- or mcr-3-bearing strains was evaluated by measuring the NPN (N-Phenylnaphthalen-1-amine: 20 μM) uptake of logarithmic phase cultures.

: N-Phenylnaphthalen-1-amine purchased from MedChemExpress. Usage Cited in: Environ Pollut. 2025 Sep 10:385:127111. [Abstract]; In the presence of PS and PLA microplastics, the uptake of NPN (N-Phenylnaphthalen-1-amine: 10 μM; 30 min) by both donor and recipient bacteria was significantly increased, indicating that the outer membrane was damaged.

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Description

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Odorant Binding Protein (OBP) Binding Affinity Assay^[1]
1. Materials Preparation
Fluorescent probe: N-phenyl-1-naphthylamine, dissolved in methanol (MeOH) to prepare a 1 mM stock solution
Protein solution: 2 μM odorant binding protein (OBP), dissolved in 50 mM Tris-HCl buffer (pH 7.4)
Experimental temperature: 25 °C
Fluorescence spectrophotometer: equipped with a 1 cm quartz cuvette, excitation and emission slit widths set to 5 nm
Detection wavelengths: Excitation wavelength (λ_ex): 337 nm; Emission wavelength (λ_em): 407 nm
2. Fluorescence Binding Assay Procedure
2.1 Place 2 μM odorant binding protein solution into the cuvette.
2.2 Gradually add the N-phenyl-1-naphthylamine stock solution (1 mM in methanol), mixing gently after each addition.
2.3 Set the final concentration range to 1-20 μM.
2.4 After each addition, immediately record the fluorescence emission spectrum (range 380-460 nm).
2.5 Measure and record the emission intensity at 407 nm for plotting the binding curve.
3. Data Analysis
3.1 Plot fluorescence intensity against the concentration of free N-phenyl-1-naphthylamine.
3.2 Use a Scatchard plot for linearization and calculation of the binding constant.
3.3 Assume a 1:1 binding ratio and 100% protein activity.
2.4 The dissociation constant (K_d) of N-phenyl-1-naphthylamine with loc-OBP1 is 1.67 μM.
4. Competitive Binding Assay
4.1 Use a fixed concentration of 2 μM odorant binding protein and 1 μM N-phenyl-1-naphthylamine.
4.2 Add test ligands (dissolved in methanol) at final concentrations typically ranging from 1-20 μM.
4.3 Monitor the decrease in fluorescence intensity and plot the competitive binding curve.
4.4 Calculate the IC₅₀, and then convert it to the dissociation constant (K_d) of the test ligand.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

219.29

Formula

C₁₆H₁₃N

CAS No.

90-30-2

Appearance

Solid

Color

Light brown to brown

SMILES

C12=CC=CC=C1C(NC3=CC=CC=C3)=CC=C2

Initial Source

Locusta migratoria

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (456.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5602 mL	22.8009 mL	45.6017 mL
5 mM	0.9120 mL	4.5602 mL	9.1203 mL
10 mM	0.4560 mL	2.2801 mL	4.5602 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Purity & Documentation

Purity: 99.98%

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Data Sheet (279 KB) SDS (761 KB)

COA (254 KB) HNMR (121 KB) RP-HPLC (227 KB) LCMS (322 KB)

Handling Instructions (2659 KB)

References

[1]. Ban L, et al., Biochemical characterization and bacterial expression of an odorant-binding protein from Locusta migratoria. Cell Mol Life Sci. 2003 Feb;60(2):390-400. [Content Brief]

[2]. Vanderlinde EM, et al. Mutation of the sensor kinase chvG in Rhizobium leguminosarum negatively impacts cellular metabolism, outer membrane stability, and symbiosis. J Bacteriol. 2012 Feb;194(4):768-77. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.5602 mL	22.8009 mL	45.6017 mL	114.0043 mL
	5 mM	0.9120 mL	4.5602 mL	9.1203 mL	22.8009 mL
	10 mM	0.4560 mL	2.2801 mL	4.5602 mL	11.4004 mL
	15 mM	0.3040 mL	1.5201 mL	3.0401 mL	7.6003 mL
	20 mM	0.2280 mL	1.1400 mL	2.2801 mL	5.7002 mL
	25 mM	0.1824 mL	0.9120 mL	1.8241 mL	4.5602 mL
	30 mM	0.1520 mL	0.7600 mL	1.5201 mL	3.8001 mL
	40 mM	0.1140 mL	0.5700 mL	1.1400 mL	2.8501 mL
	50 mM	0.0912 mL	0.4560 mL	0.9120 mL	2.2801 mL
	60 mM	0.0760 mL	0.3800 mL	0.7600 mL	1.9001 mL
	80 mM	0.0570 mL	0.2850 mL	0.5700 mL	1.4251 mL
	100 mM	0.0456 mL	0.2280 mL	0.4560 mL	1.1400 mL