Cat. No.: HY-B0965

Thioridazine hydrochloride

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Thioridazine hydrochloride, an orally active antagonist of the dopamine receptor D2 family proteins, exhibits potent anti-psychotic and anti-anxiety activities. Thioridazine hydrochloride is also a potent inhibitor of PI3K-Akt-mTOR signaling pathways with anti-angiogenic effect. Thioridazine hydrochloride shows antiproliferative and apoptosis induction effects in various types of cancer cells, with specificity on targeting cancer stem cells (CSCs).

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 130-61-0
Formula: C₂₁H₂₇ClN₂S₂
Molecular Weight:407.04

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Thioridazine hydrochloride Chemical Structure

CAS No.: 130-61-0

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

serotonin

All Dopamine Receptor Isoforms :

All 5-HT Receptor Isoforms :

In Vitro

Thioridazine (0.01-100 μM; 48 h) reduces the cell viability of NCI-N87 and AGS cells in a concentration-dependent manner^[2].
Thioridazine (15 μM; 24 h) reduces cell viability of the cervical (HeLa, Caski and C33A) and endometrial (HEC-1-A and KLE) cancer cells^[4].
Thioridazine (1-15 μM; 24-48 h) induces gastric cancer cell death via the mitochondrial apoptosis pathway and mitochondrial pathway^[2].
Thioridazine (15 μM; 24 h) modulates the regulation of cell cycle progression by interfering with the PI3K/Akt pathway and induces G₁ cell cycle arrest in cervical and endometrial cancer cells ^[4].
Thioridazine inhibits the growth of antibiotic-sensitive and multidrug-resistant strains of A. baumannii^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

NCI-N87 and AGS cells
Concentration:

0.01, 0.1, 0.5, 1, 5, 10, 20, 50, 100 μM
Incubation Time:

48 hours
Result:

Exhibited cytotoxicity in gastric cancer cells.

Western Blot Analysis^[1]

Cell Line:

NCI-N87 and AGS cells
Concentration:

1, 5, 10, 15 μM
Incubation Time:

24, 48 hours
Result:

Downregulated the precursors of caspase-9, caspase-8 and caspase-3.

In Vivo

Thioridazine (25 mg/kg; i.p. every 3 days for 3 weeks) extends the survival of tumor-bearing mice and reduces the number of pluripotent embryonal carcinoma (EC) cells within tumors^[5].
Thioridazine (1.0-5.0 mg/kg; s.c.) reduces oral behavior and selectively blocks repetitive head bobbing^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Nude and Rag2KO mice were injected with iPS cells or NT2D1 cells^[5]
Dosage:

25 mg/kg
Administration:

I.p. every 3 days for 3 weeks
Result:

Reduced the number of OCT4-expressing cells within malignant teratocarcinomas and extended the survival of tumor-bearing mice.
With no effect on fertility.

Chemical Information

CAS No. 130-61-0
Appearance Solid
Molecular Weight 407.04
Formula C₂₁H₂₇ClN₂S₂
Color White to off-white
SMILES

CSC(C=C1N2CCC3N(C)CCCC3)=CC=C1SC4=C2C=CC=C4.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (6)

All Cell Proliferation/Viability AssayIn Vivo Efficacy StudyMicrobiological Assay

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Journal Impact Factor

Int J Biol Macromol

The combination of cloxacillin, thioridazine and tetracycline protects mice against Staphylococcus aureus peritonitis by inhibiting α-Hemolysin-induced MAPK/NF-κB/NLRP3 activation. [Abstract]

2022 Feb 15:198:1-10. PMID: 34963621
Br J Cancer

DRD4 promotes chemo-resistance and cancer stem cell-like phenotypes by mediating the activation of the Akt/β-catenin signaling axis in liver cancer. [Abstract]

2024 Oct;131(7):1212-1223. PMID: 39174739

Thioridazine hydrochloride purchased from MedChemExpress. Usage Cited in: Br J Cancer. 2024 Oct;131(7):1212-1223. [Abstract]

HepG2-R cells were seeded at a density of 3 × 10³ cells per well in 96-well plates and cultured overnight. The cells were exposed to 250 nM and 500 nM adriamycin (ADR) and 20 μM Thioridazine hydrochloride (TDZ) either individually or in combination for a duration of 48 h. The viability of these cells was assessed using an MTS assay, measuring the optical density at 490 nm (OD490 value).
Int J Mol Sci

Miltefosine and Nifuratel Combination: A Promising Therapy for the Treatment of Leishmania donovani Visceral Leishmaniasis. [Abstract]

2023 Jan 13;24(2):1635. PMID: 36675150
Chin J Integr Med

Platycodin D Overcomes Cisplatin Resistance in Gastric Cancer by Inducing Ferroptosis through TLK1-Dependent UHRF2/DNMT3A/ALOX15 Pathway. [Abstract]

2026 May 18. PMID: 42149324
Pol J Microbiol

Inhibition of Drug Resistance of Staphylococcus aureus by Efflux Pump Inhibitor and Autolysis Inducer to Strengthen the Antibacterial Activity of β-lactam Drugs. [Abstract]

2019 Dec;68(4):477-491. PMID: 31880892

Thioridazine hydrochloride purchased from MedChemExpress. Usage Cited in: Pol J Microbiol. 2019 Dec;68(4):477-491. [Abstract]

In the MRSA-infected groups, the mammary glands varied in color; those of the blank control group were milky white, those of the infection control group were purple, those of the MCZ or Thioridazine hydrochloride (TZ, 12 and 16 mg/kg) treated group were red, those of a portion of the CXN treated group were red but most were milky white, and most were also milky white in the group treated with the combination of the three drugs.

Thioridazine hydrochloride purchased from MedChemExpress. Usage Cited in: Pol J Microbiol. 2019 Dec;68(4):477-491. [Abstract]

Colony counts from mice of the clinical strain MRSA135-infected group showed that the bacterial concentration was 6.96 × 10⁷ CFU/ml without therapy. Following treatment, the bacterial concentration in mice of the MCZ-treatment group was 2.23 × 10⁷ CFU/ml; in the Thioridazine hydrochloride (TZ, 4 μg/mL)-treatment group it was 1.73 × 10⁷ CFU/ml; after CXN treatment it was 3.20 × 10⁶ CFU/ml (p = 0.0447 < 0.05), and after the combination therapy with CXN, TZ, and MCZ it was 1.10 × 10⁶ CFU/ml (p = 0.0427 < 0.05).

Thioridazine hydrochloride purchased from MedChemExpress. Usage Cited in: Pol J Microbiol. 2019 Dec;68(4):477-491. [Abstract]

The MICs values of individual antimicrobial agents against Staphylococcus aureus isolates.
bioRxiv

Drug-induced phospholipidosis as an artifact in antiviral drug repurposing. [Abstract]

2025 Nov 21:2025.11.20.689520. PMID: 41332603

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (245.68 mM; Need ultrasonic)

DMSO : ≥ 45 mg/mL (110.55 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4568 mL

12.2838 mL

24.5676 mL

5 mM

0.4914 mL

2.4568 mL

4.9135 mL

10 mM

0.2457 mL

1.2284 mL

2.4568 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 12.5 mg/mL (30.71 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tschanz JT, et, al. Atypical antipsychotic drugs block selective components of amphetamine-induced stereotypy. Pharmacol Biochem Behav. 1988 Nov;31(3):519-22. [Content Brief]

[2]. Mu J, et, al. Thioridazine, an antipsychotic drug, elicits potent antitumor effects in gastric cancer. Oncol Rep. 2014 May;31(5):2107-14. [Content Brief]

[3]. Aguilar-Vega L, et, al. Antibacterial properties of phenothiazine derivatives against multidrug-resistant Acinetobacter baumannii strains. J Appl Microbiol. 2021 Apr 22. [Content Brief]

[4]. Kang S, et, al. Thioridazine induces apoptosis by targeting the PI3K/Akt/mTOR pathway in cervical and endometrial cancer cells. Apoptosis. 2012 Sep;17(9):989-97. [Content Brief]

[5]. Loehr AR, et, al. Targeting Cancer Stem Cells with Differentiation Agents as an Alternative to Genotoxic Chemotherapy for the Treatment of Malignant Testicular Germ Cell Tumors. Cancers (Basel). 2021 Apr 23;13(9):2045. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	2.4568 mL	12.2838 mL	24.5676 mL	61.4190 mL
	5 mM	0.4914 mL	2.4568 mL	4.9135 mL	12.2838 mL
	10 mM	0.2457 mL	1.2284 mL	2.4568 mL	6.1419 mL
	15 mM	0.1638 mL	0.8189 mL	1.6378 mL	4.0946 mL
	20 mM	0.1228 mL	0.6142 mL	1.2284 mL	3.0710 mL
	25 mM	0.0983 mL	0.4914 mL	0.9827 mL	2.4568 mL
	30 mM	0.0819 mL	0.4095 mL	0.8189 mL	2.0473 mL
	40 mM	0.0614 mL	0.3071 mL	0.6142 mL	1.5355 mL
	50 mM	0.0491 mL	0.2457 mL	0.4914 mL	1.2284 mL
	60 mM	0.0409 mL	0.2047 mL	0.4095 mL	1.0237 mL
	80 mM	0.0307 mL	0.1535 mL	0.3071 mL	0.7677 mL
	100 mM	0.0246 mL	0.1228 mL	0.2457 mL	0.6142 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Thioridazine hydrochloride

6 Cited Publications

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Thioridazine hydrochloride

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Bioactive Compound Library

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Publications Citing Use of MedChemExpress (MCE) Thioridazine hydrochloride

All Dopamine Receptor Isoforms

All 5-HT Receptor Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Thioridazine hydrochloride Related Classifications

HY-B0965 Related Classifications