Cat. No.: HY-B0965A

Thioridazine

5 Cited Publications

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Based on 5 publication(s) in Google Scholar

Thioridazine, an antagonist of the dopamine receptor D2 family proteins, exhibits potent anti-psychotic and anti-anxiety activities. Thioridazine is also a potent inhibitor of PI3K-Akt-mTOR signaling pathways with anti-angiogenic effect. Thioridazine shows antiproliferative and apoptosis induction effects in various types of cancer cells, with specificity on targeting cancer stem cells (CSCs).

For research use only. We do not sell to patients.

Purity: 99.50%
CAS No.: 50-52-2
Formula: C₂₁H₂₆N₂S₂
Molecular Weight:370.57

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Thioridazine Chemical Structure

CAS No.: 50-52-2

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Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All Dopamine Receptor Isoforms :

All 5-HT Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	3.5 μM Compound: thioridazine	Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits	[PMID: 23812503]
HBL1	GI₅₀	6.6 μM Compound: Thioridazine	Antiproliferative activity against human HBL1 cells assessed as inhibition of cell growth incubated for 72 hrs by Celltiter-Glo luminescent assay Antiproliferative activity against human HBL1 cells assessed as inhibition of cell growth incubated for 72 hrs by Celltiter-Glo luminescent assay	[PMID: 34181850]
HEK293	IC₅₀	1.32 μM Compound: Thioridazine	Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit	[PMID: 22761000]
HEK293	IC₅₀	1830 nM Compound: Thioridazine	Inhibition of sodium current measured using whole-cell patch clamp experiments in HEK-293 cells stably transfected with hNaV1.5 cDNA Inhibition of sodium current measured using whole-cell patch clamp experiments in HEK-293 cells stably transfected with hNaV1.5 cDNA	[PMID: 21300721]
J774	IC₅₀	4.1 μg/mL Compound: 1; TZ	Cytotoxicity against mouse J774 cells assessed as decrease in cell viability after 24 hrs by resazurin reduction assay Cytotoxicity against mouse J774 cells assessed as decrease in cell viability after 24 hrs by resazurin reduction assay	[PMID: 28039773]
KG-1a	IC₅₀	6.01 μM Compound: THD	Antiproliferative activity against human KG1a cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human KG1a cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
L5178Y	IC₅₀	12.72 nM Compound: TZ	Cytotoxicity against mouse L5178Y cells assessed as reduction in cell viability by MTT assay Cytotoxicity against mouse L5178Y cells assessed as reduction in cell viability by MTT assay	[PMID: 32871268]
L5178Y	IC₅₀	12.72 μM Compound: TZ	Cytotoxicity against mouse L5178Y cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against mouse L5178Y cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 27156771]
L5178Y	IC₅₀	7.43 nM Compound: TZ	Cytotoxicity against multidrug resistance mouse L5178Y cells expressing human MDR1 assessed as reduction in cell viability by MTT assay Cytotoxicity against multidrug resistance mouse L5178Y cells expressing human MDR1 assessed as reduction in cell viability by MTT assay	[PMID: 32871268]
L5178Y	IC₅₀	7.43 μM Compound: TZ	Cytotoxicity against multi-drug resistant mouse L5178Y cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against multi-drug resistant mouse L5178Y cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 27156771]
Macrophage cell line	CC₅₀	13.8 μM Compound: TZ	Cytotoxicity in human monocyte-derived macrophages assessed as reduction in cell viability incubated for 3 days by alamar blue dye based assay Cytotoxicity in human monocyte-derived macrophages assessed as reduction in cell viability incubated for 3 days by alamar blue dye based assay	[PMID: 28964936]
MCF7	IC₅₀	12.95 μM Compound: THD	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
MDA-MB-231	IC₅₀	14.19 μM Compound: THD	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
MRC5	IC₅₀	8.2 μg/mL Compound: 1; TZ	Cytotoxicity against human MRC5 cells assessed as decrease in cell viability after 24 hrs by resazurin reduction assay Cytotoxicity against human MRC5 cells assessed as decrease in cell viability after 24 hrs by resazurin reduction assay	[PMID: 28039773]
OCI-Ly1	GI₅₀	13.4 μM Compound: Thioridazine	Antiproliferative activity against human OCI-Ly1 cells assessed as inhibition of cell growth incubated for 72 hrs by Celltiter-Glo luminescent assay Antiproliferative activity against human OCI-Ly1 cells assessed as inhibition of cell growth incubated for 72 hrs by Celltiter-Glo luminescent assay	[PMID: 34181850]
PBMC	IC₅₀	13.78 μM Compound: TZ	Cytotoxicity against human PBMC assessed as cell viability after 3 days by AlamarBlue assay Cytotoxicity against human PBMC assessed as cell viability after 3 days by AlamarBlue assay	[PMID: 26197353]
SK-BR-3	IC₅₀	18.23 μM Compound: THD	Antiproliferative activity against human SKBR3 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human SKBR3 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
SUM-159-PT	IC₅₀	14.51 μM Compound: THD	Antiproliferative activity against human SUM159 cells assessed as reduction in cell viability incubated for 2 days by MTT assay Antiproliferative activity against human SUM159 cells assessed as reduction in cell viability incubated for 2 days by MTT assay	[PMID: 31541872]
TMD8	GI₅₀	5.9 μM Compound: Thioridazine	Antiproliferative activity against human TMD8 cells assessed as inhibition of cell growth incubated for 72 hrs by Celltiter-Glo luminescent assay Antiproliferative activity against human TMD8 cells assessed as inhibition of cell growth incubated for 72 hrs by Celltiter-Glo luminescent assay	[PMID: 34181850]
Ventricular myocyte	IC₅₀	1.3 μM Compound: Thioridazine	Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes	[PMID: 22761000]
Ventricular myocyte	IC₅₀	1300 nM Compound: Thioridazine	Inhibition of calcium current (ICaL) measured using whole-cell patch clamp experiments in isolated guinea pig ventricular myocytes Inhibition of calcium current (ICaL) measured using whole-cell patch clamp experiments in isolated guinea pig ventricular myocytes	[PMID: 21300721]
Vero	CC₅₀	15.96 μM Compound: Thioridazine	Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr	10.1101/2020.03.20.999730
Vero	IC₅₀	17.79 μg/mL Compound: Tz	Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability incubated for 72 hrs by resazurin assay Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability incubated for 72 hrs by resazurin assay	[PMID: 32526553]
Vero	IC₅₀	5.3 μg/mL Compound: thioridazine	Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability by MTT assay Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability by MTT assay	[PMID: 25238443]
Vero	IC₅₀	6.69 μM Compound: Thioridazine	Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr	10.1101/2020.03.20.999730
Vero C1008	IC₅₀	6.24 μM Compound: Thioridazine	Antiviral activity against Ebolavirus expressing eGFP infected in African green monkey Vero E6 cells assessed as inhibition of viral replication after 48 hrs by spectrofluorometric method Antiviral activity against Ebolavirus expressing eGFP infected in African green monkey Vero E6 cells assessed as inhibition of viral replication after 48 hrs by spectrofluorometric method	[PMID: 29741894]

In Vitro

Thioridazine (0.01-100 μM; 48 h) reduces the cell viability of NCI-N87 and AGS cells in a concentration-dependent manner^[2].
Thioridazine (15 μM; 24 h) reduces cell viability of the cervical (HeLa, Caski and C33A) and endometrial (HEC-1-A and KLE) cancer cells^[4].
Thioridazine (1-15 μM; 24-48 h) induces gastric cancer cell death via the mitochondrial apoptosis pathway and mitochondrial pathway^[2].
Thioridazine (15 μM; 24 h) modulates the regulation of cell cycle progression by interfering with the PI3K/Akt pathway and induces G₁ cell cycle arrest in cervical and endometrial cancer cells ^[4].
Thioridazine inhibits the growth of antibiotic-sensitive and multidrug-resistant strains of A. baumannii^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

NCI-N87 and AGS cells.
Concentration:

0.01, 0.1, 0.5, 1, 5, 10, 20, 50, 100 μM.
Incubation Time:

48 hours.
Result:

Exhibited cytotoxicity in gastric cancer cells.

Western Blot Analysis^[1]

Cell Line:

NCI-N87 and AGS cells
Concentration:

1, 5, 10, 15 μM.
Incubation Time:

24, 48 hours.
Result:

Downregulated the precursors of caspase-9, caspase-8 and caspase-3.

In Vivo

Thioridazine (25 mg/kg; i.p. every 3 days for 3 weeks) extends the survival of tumor-bearing mice and reduces the number of pluripotent embryonal carcinoma (EC) cells within tumors^[5].
Thioridazine (1.0-5.0 mg/kg; s.c.) reduces oral behavior and selectively blocks repetitive head bobbing^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Nude and Rag2KO mice were injected with iPS cells or NT2D1 cells^[5]
Dosage:

25 mg/kg.
Administration:

I.p. every 3 days for 3 weeks.
Result:

Reduced the number of OCT4-expressing cells within malignant teratocarcinomas and extended the survival of tumor-bearing mice.
With no effect on fertility.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 50-52-2
Appearance Oil
Molecular Weight 370.57
Formula C₂₁H₂₆N₂S₂
Color Colorless to off-white
SMILES

CSC(C=C1N2CCC3N(C)CCCC3)=CC=C1SC4=C2C=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (5)

All Cell Proliferation/Viability AssayIn Vivo Efficacy StudyMicrobiological Assay

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Journal Impact Factor

Int J Biol Macromol

The combination of cloxacillin, thioridazine and tetracycline protects mice against Staphylococcus aureus peritonitis by inhibiting α-Hemolysin-induced MAPK/NF-κB/NLRP3 activation. [Abstract]

2022 Feb 15:198:1-10. PMID: 34963621
Br J Cancer

DRD4 promotes chemo-resistance and cancer stem cell-like phenotypes by mediating the activation of the Akt/β-catenin signaling axis in liver cancer. [Abstract]

2024 Oct;131(7):1212-1223. PMID: 39174739

Thioridazine purchased from MedChemExpress. Usage Cited in: Br J Cancer. 2024 Oct;131(7):1212-1223. [Abstract]

HepG2-R cells were seeded at a density of 3 × 10³ cells per well in 96-well plates and cultured overnight. The cells were exposed to 250 nM and 500 nM adriamycin (ADR) and 20 μM Thioridazine hydrochloride (TDZ) either individually or in combination for a duration of 48 h. The viability of these cells was assessed using an MTS assay, measuring the optical density at 490 nm (OD490 value).
Int J Mol Sci

Miltefosine and Nifuratel Combination: A Promising Therapy for the Treatment of Leishmania donovani Visceral Leishmaniasis. [Abstract]

2023 Jan 13;24(2):1635. PMID: 36675150
Pol J Microbiol

Inhibition of Drug Resistance of Staphylococcus aureus by Efflux Pump Inhibitor and Autolysis Inducer to Strengthen the Antibacterial Activity of β-lactam Drugs. [Abstract]

2019 Dec;68(4):477-491. PMID: 31880892

Thioridazine purchased from MedChemExpress. Usage Cited in: Pol J Microbiol. 2019 Dec;68(4):477-491. [Abstract]

In the MRSA-infected groups, the mammary glands varied in color; those of the blank control group were milky white, those of the infection control group were purple, those of the MCZ or Thioridazine hydrochloride (TZ, 12 and 16 mg/kg) treated group were red, those of a portion of the CXN treated group were red but most were milky white, and most were also milky white in the group treated with the combination of the three drugs.

Thioridazine purchased from MedChemExpress. Usage Cited in: Pol J Microbiol. 2019 Dec;68(4):477-491. [Abstract]

Colony counts from mice of the clinical strain MRSA135-infected group showed that the bacterial concentration was 6.96 × 10⁷ CFU/ml without therapy. Following treatment, the bacterial concentration in mice of the MCZ-treatment group was 2.23 × 10⁷ CFU/ml; in the Thioridazine hydrochloride (TZ, 4 μg/mL)-treatment group it was 1.73 × 10⁷ CFU/ml; after CXN treatment it was 3.20 × 10⁶ CFU/ml (p = 0.0447 < 0.05), and after the combination therapy with CXN, TZ, and MCZ it was 1.10 × 10⁶ CFU/ml (p = 0.0427 < 0.05).

Thioridazine purchased from MedChemExpress. Usage Cited in: Pol J Microbiol. 2019 Dec;68(4):477-491. [Abstract]

The MICs values of individual antimicrobial agents against Staphylococcus aureus isolates.
bioRxiv

Drug-induced phospholipidosis as an artifact in antiviral drug repurposing. [Abstract]

2025 Nov 21:2025.11.20.689520. PMID: 41332603

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (269.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6985 mL

13.4927 mL

26.9855 mL

5 mM

0.5397 mL

2.6985 mL

5.3971 mL

10 mM

0.2699 mL

1.3493 mL

2.6985 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tschanz JT, et, al. Atypical antipsychotic drugs block selective components of amphetamine-induced stereotypy. Pharmacol Biochem Behav. 1988 Nov;31(3):519-22. [Content Brief]

[2]. Mu J, et, al. Thioridazine, an antipsychotic drug, elicits potent antitumor effects in gastric cancer. Oncol Rep. 2014 May;31(5):2107-14. [Content Brief]

[3]. Kang S, et, al. Thioridazine induces apoptosis by targeting the PI3K/Akt/mTOR pathway in cervical and endometrial cancer cells. Apoptosis. 2012 Sep;17(9):989-97. [Content Brief]

[4]. Loehr AR, et, al. Targeting Cancer Stem Cells with Differentiation Agents as an Alternative to Genotoxic Chemotherapy for the Treatment of Malignant Testicular Germ Cell Tumors. Cancers (Basel). 2021 Apr 23;13(9):2045. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6985 mL	13.4927 mL	26.9855 mL	67.4636 mL
	5 mM	0.5397 mL	2.6985 mL	5.3971 mL	13.4927 mL
	10 mM	0.2699 mL	1.3493 mL	2.6985 mL	6.7464 mL
	15 mM	0.1799 mL	0.8995 mL	1.7990 mL	4.4976 mL
	20 mM	0.1349 mL	0.6746 mL	1.3493 mL	3.3732 mL
	25 mM	0.1079 mL	0.5397 mL	1.0794 mL	2.6985 mL
	30 mM	0.0900 mL	0.4498 mL	0.8995 mL	2.2488 mL
	40 mM	0.0675 mL	0.3373 mL	0.6746 mL	1.6866 mL
	50 mM	0.0540 mL	0.2699 mL	0.5397 mL	1.3493 mL
	60 mM	0.0450 mL	0.2249 mL	0.4498 mL	1.1244 mL
	80 mM	0.0337 mL	0.1687 mL	0.3373 mL	0.8433 mL
	100 mM	0.0270 mL	0.1349 mL	0.2699 mL	0.6746 mL

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Thioridazine

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Publications Citing Use of MedChemExpress (MCE) Thioridazine

All Dopamine Receptor Isoforms

All 5-HT Receptor Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Thioridazine Related Classifications

Cat. No.: HY-B0965A