1. Apoptosis
  2. Apoptosis
  3. Ubiquitin Isopeptidase Inhibitor I, G5

Ubiquitin Isopeptidase Inhibitor I, G5  (Synonyms: NSC144303)

Cat. No.: HY-100738 Purity: ≥98.0%
COA Handling Instructions

Ubiquitin Isopeptidase Inhibitor I, G5 (NSC 144303) is an apoptosome-independent caspase and apoptosis activator with IC50 values of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively.

For research use only. We do not sell to patients.

Ubiquitin Isopeptidase Inhibitor I, G5 Chemical Structure

Ubiquitin Isopeptidase Inhibitor I, G5 Chemical Structure

CAS No. : 108477-18-5

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
5 mg USD 92 In-stock
10 mg USD 158 In-stock
25 mg USD 310 In-stock
50 mg USD 500 In-stock
100 mg USD 800 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Ubiquitin Isopeptidase Inhibitor I, G5 (NSC 144303) is an apoptosome-independent caspase and apoptosis activator with IC50 values of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively.

IC50 & Target

IC50: 1.76 μM (E1A cells), 1.6 μM (E1A/C9DN cells)[1]

In Vitro

G5 is capable of activating an apoptosome-independent apoptotic pathway. It targets the ubiquitinproteasome system by inhibiting the ubiquitin isopeptidases. G5 induces a rather unique apoptotic pathway, which includes a Bcl-2-dependent but apoptosome-independent mitochondrial pathway with upregulation of the BH3-only protein Noxa, stabilization of the inhibitor of apoptosis antagonist Smac, but also the involvement of the death receptor pathway. G5 is a potent apoptotic inducer showing IC50s of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

414.39

Formula

C19H14N2O7S

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(/C(C1)=C/C2=CC=C([N+]([O-])=O)C=C2)/C(CS1(=O)=O)=C/C3=CC=C([N+]([O-])=O)C=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (120.66 mM; Need ultrasonic)

THF : 3.44 mg/mL (8.30 mM; Need ultrasonic)

Acetone : 2.86 mg/mL (6.90 mM; Need ultrasonic)

Ethanol : < 1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4132 mL 12.0659 mL 24.1319 mL
5 mM 0.4826 mL 2.4132 mL 4.8264 mL
10 mM 0.2413 mL 1.2066 mL 2.4132 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  15% Cremophor EL    85% Saline

    Solubility: 10 mg/mL (24.13 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation
References
Cell Assay
[1]

Different drug concentrations are used to determine the dose-response profile and to calculate the IC50 value. Cells are incubated with the required concentrations of the 57 compounds. After 24 or 48 hours of incubation, cell death is evaluated bytr ypan blue staining[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Ubiquitin Isopeptidase Inhibitor I, G5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Ubiquitin Isopeptidase Inhibitor I, G5
Cat. No.:
HY-100738
Quantity:
MCE Japan Authorized Agent: