1. Cell Cycle/DNA Damage
    Apoptosis
  2. Topoisomerase
    Apoptosis
  3. (S)-10-Hydroxycamptothecin

(S)-10-Hydroxycamptothecin (Synonyms: 10-HCPT; 10-Hydroxycamptothecin)

Cat. No.: HY-N0095 Purity: 99.38%
Handling Instructions

(S)-10-Hydroxycamptothecin (10-HCPT;10-Hydroxycamptothecin) is a DNA topoisomerase I inhibitor of isolated from the Chinese plant Camptotheca accuminata. (S)-10-Hydroxycamptothecin exhibits a remarkable apoptosis-inducing effect. (S)-10-Hydroxycamptothecin has the potential for hepatoma, gastric carcinoma, colon cancer and leukaemia treatment.

For research use only. We do not sell to patients.

(S)-10-Hydroxycamptothecin Chemical Structure

(S)-10-Hydroxycamptothecin Chemical Structure

CAS No. : 19685-09-7

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Solution
10 mM * 1 mL in DMSO USD 79 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 79 In-stock
Estimated Time of Arrival: December 31
Solid
50 mg USD 72 In-stock
Estimated Time of Arrival: December 31
100 mg USD 108 In-stock
Estimated Time of Arrival: December 31
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Description

(S)-10-Hydroxycamptothecin (10-HCPT;10-Hydroxycamptothecin) is a DNA topoisomerase I inhibitor of isolated from the Chinese plant Camptotheca accuminata. (S)-10-Hydroxycamptothecin exhibits a remarkable apoptosis-inducing effect. (S)-10-Hydroxycamptothecin has the potential for hepatoma, gastric carcinoma, colon cancer and leukaemia treatment[1][2][3][4].

IC50 & Target

Topoisomerase I

 

In Vitro

(S)-10-Hydroxycamptothecin (5-20 µg/L; 6 days; Hep G2 cells) treatment results in the cell cycle arrest at G2/M phase[2].
(S)-10-Hydroxycamptothecin induces differentiation, down-regulates nuclear antigen (PCNA) and up-regulates wild-type protein p53 in Hep G2 cells[2].
(S)-10-Hydroxycamptothecin inhibits L1210 leukemia cells with an IC50 of 1.15 µM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis[2]

Cell Line: Hep G2 cells
Concentration: 5 µg/L, 10 µg/L, 20 µg/L
Incubation Time: 6 days
Result: Hep G2 cells were mainly arrested at G2/M phase.
In Vivo

(S)-10-Hydroxycamptothecin (10-Hydroxycamptothecin) against L1210 leukemia in mice is tested. At the optimal dose (15 mg/kg), (S)-10-Hydroxycamptothecin has a 71% increase in life span (ILS)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

364.35

Formula

C₂₀H₁₆N₂O₅

CAS No.
SMILES

O=C1[[email protected]](O)(CC)C2=C(CO1)C(N3CC4=CC5=CC(O)=CC=C5N=C4C3=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (137.23 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7446 mL 13.7231 mL 27.4461 mL
5 mM 0.5489 mL 2.7446 mL 5.4892 mL
10 mM 0.2745 mL 1.3723 mL 2.7446 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (6.86 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (6.86 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References

Purity: 99.38%

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Keywords:

(S)-10-Hydroxycamptothecin10-HCPT 10-HydroxycamptothecinTopoisomeraseApoptosisCell-cyclearrestG2/MantitumorPCNAp53c-MycBcl-2Baxcaspase-1/3 rInhibitorinhibitorinhibit

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(S)-10-Hydroxycamptothecin
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