AF 488 maleimide
Based on 1 Customer Validation
AF 488 maleimide is a thiol-reactive dye used to label SH groups of proteins, which can attach the AF 488 fluorophore to cysteine residue-containing proteins and peptides as well as other thiolated molecules. AF 488 maleimide enables real-time visualization of dynamic pilus extension and retraction in live bacterial cells via epifluorescence microscopy (Ex/Em = 470/520 nm).
For research use only. We do not sell to patients.
- Purity: 96.61%
- Formula: C31H27N4NaO12S2
- Molecular Weight:734.69
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
AF 488 maleimide (25 μg/mL; 5-30 min) brightly labels type IVa competence pili in Vibrio cholerae PilAS67C cells, enabling real-time visualization of dynamic pilus extension and retraction via epifluorescence microscopy[1].
AF 488 maleimide (25 μg/mL) brightly labels type IVa MSHA pili in Vibrio cholerae MshAS156C and MshAT81C cells, enabling visualization via single-time-point epifluorescence microscopy[1].
AF 488 maleimide (25 μg/mL) brightly labels type IVb TCP pili in Vibrio cholerae TcpAT184C cells, enabling visualization via single-time-point epifluorescence microscopy[1].
AF 488 maleimide (25 μg/mL; 5-30 min) brightly labels type IVc Tad pili in Caulobacter crescentus PilAT36C cells, enabling real-time visualization of dynamic pilus activity, and when used alongside pre-treatment with PEG5000-mal, allows visualization of static, retraction-blocked pili via epifluorescence microscopy[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 734.69
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Formula C31H27N4NaO12S2
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Color Orange to red
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SMILES
NC(C=C1)=C(S(=O)(O[Na])=O)C2=C1C(C3=C(C(O)=O)C=C(C(NCCCCCCN4C(C=CC4=O)=O)=O)C=C3)=C(C=C5)C(O2)=C(S(=O)([O-])=O)C5=[NH2+]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 3.67 mg/mL (5.00 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3611 mL | 6.8056 mL | 13.6112 mL | 34.0280 mL |
AF 488 maleimide Related Classifications
HY-D2381 Related Classifications
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