ALK-IN-23 

ALK-IN-23 is a potent ALK inhibitor with IC₅₀ values of 1.6 nM, 0.71 nM and 1.3 nM for ALK^WT, ALK^L1196M and ALK^G1202R. ALK-IN-23 can block cell cycle in G2 phase and induce apoptosis. ALK-IN-23 inhibits cancer cell migration and colony formation in vitro. ALK-IN-23 exhibits antitumor activity in H2228 xenograft nude mice model with hypotoxicity^[1].

IC₅₀: 1.6 nM (ALK^WT), 0.71 nM (ALK^L1196M), 1.3 nM (ALK^G1202R)^[1]

ALK-IN-23 (compound Y28) (0-5 μM; 72h) has highly inhibitory activity against H3122, H2228, Karpas299 and A549^[1].
ALK-IN-23 (25-100 nM; 3 days) clearly reduces the number of H2228 cell colonies, and almost completely abolishes the formation of colonies at 100 nM^[1].
ALK-IN-23 (100-200 nM; 48 h) facilitates the apoptosis of H2228 cells^[1].
ALK-IN-23 (5-10 nM; 24 and 48 h) is effective to block the migration of most cells at a dose of 10 nM^[1].
ALK-IN-23 (25-100 nM; overnight) significantly increases the percentage of cells in the G2 phase^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ALK-IN-23 notes a moderate half-life of 16.3 min and a high intrinsic liver clearance of 152.9 mL/min/kg in rats^[1].
ALK-IN-23 (25 and 50 mg/kg; IG; once every 2 days; for 14 days) exhibited gentle antitumor efficacy and no significant weight loss in H2228 xenograft mice model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

569.08

C₂₆H₂₉ClN₈O₃S

3033549-18-4

CC(S(=O)(C1=CC=CC=C1NC2=NC(NC3=CC=C4C(NN=C4NC(CN5CCCC5)=O)=C3)=NC=C2Cl)=O)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Yang J, et al. Design, synthesis and antitumor evaluation of ATP dual-mimic 2,4-diarylaminopyrimidine and aminoindazole conjugates as potent anaplastic lymphoma kinase inhibitors. Eur J Med Chem. 2022 Jul 31;241:114626.  [Content Brief]