Anti-Mouse CD172a Antibody (P84)
Based on 1 Customer Validation
Anti-Mouse CD172a Antibody (P84) is a rat-derived anti-mouse CD172a IgG1 κ type antibody inhibitor. Anti-Mouse CD172a Antibody (P84) blocks CD47-SIRPα interaction and thereby augments cell phagocytosis. Anti-Mouse CD172a Antibody (P84) can be used for the researches of infection and immunology, such as salmonella typhimurium infection.
For research use only. We do not sell to patients.
- Purity: 95.00%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Rat IgG1 kappa
Mouse
CD172a
Anti-Mouse CD172a Antibody (P84) (20 mins) significantly increases the engulfment of WT mouse RBCs[1].
Anti-Mouse CD172a Antibody (P84) (5 μg/mL, 30 mins) enhances the phagocytic incorporation of salmonella typhimurium into Nr2f6-deficient macrophages in splenocytes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Anti-Mouse CD172a Antibody (P84) (i.p., 24 h before cells injection) prevents the effects of mouse CD47 on macrophage-mediated xenograft rejection in diabetic Rag22/2 c chain 2/2 mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Salmonella typhimurium infectious mice models (Nr2f6-deficient mice)[2]
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Dosage:100 μg
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Administration:Intraperitoneally injection, one day prior to infection and at days 1 and 2 post-infection
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Result:Showed body weight loss.
Increased splenic and liver bacterial counts.
Increased CD172a-positive cells and abolished Sirp𝛼 (CD172a)-positive CD45+ blood cells.
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Animal Model:The diabetic Rag22/2 c chain 2/2 mice were injected by mCD47-INS-1E or cont-INS-1E cells or CFSE-labeled congenic T cells[3]
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Dosage:/
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Administration:Intraperitoneally injection, 24 h before cells injection
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Result:Failed to achieve normoglycemia.
Did not induce phagocytosis of congenic cells.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
in vivo SIRPα blocking; In vitro SIRPα blocking; Western blot; Immunoprecipitation; Flow cytomet
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Immobilized SIRP alpha/CD172a Protein, Mouse (HEK293, His, HY-P72468) can bind Anti-Mouse CD172a Antibody (P84). The EC50 for this effect is 99.62 ng/mL.
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse CD172a Antibody (P84)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Wang H, et al. Attenuation of phagocytosis of xenogeneic cells by manipulating CD47. Blood. 2007 Jan 15;109(2):836-42. [Content Brief]
[2]. Woelk J, et al. Loss of NR2F6 Protects from Salmonella Typhimurium Infection. Adv Sci (Weinh). 2025 Jul 2:e04280. [Content Brief]
[3]. eraoka Y, et al. Expression of recipient CD47 on rat insulinoma cell xenografts prevents macrophage-mediated rejection through SIRPα inhibitory signaling in mice. PLoS One. 2013;8(3):e58359. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CD172a Antibody (P84) Related Classifications
HY-P990289 Related Classifications
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