Anti-Mouse CD47/IAP Antibody (MIAP301)
Based on 2 publication(s) in Google Scholar
Anti-Mouse CD47/IAP Antibody (MIAP301) is an anti-mouse CD47/IAP IgG2a monoclonal antibody. Anti-Mouse CD47/IAP Antibody (MIAP301) can effectively block CD47 signaling and enhance macrophage phagocytic function. Anti-Mouse CD47/IAP Antibody (MIAP301) can increase the infiltration of immune cells. Anti-Mouse CD47/IAP Antibody (MIAP301) restores the phagocytic function of myeloid cells and alleviate B cell inhibition. Anti-Mouse CD47/IAP Antibody (MIAP301) may interfere with wound healing. Anti-Mouse CD47/IAP Antibody (MIAP301) can be used for researches on cancer, inflammation and infection conditions such as melanoma, intestinal mucosal repair and sepsis.
For research use only. We do not sell to patients.
- Purity : 95.00%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Anti-Mouse CD47/IAP Antibody (MIAP301)
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Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
CD47/IAP
In Vitro
Anti-Mouse CD47/IAP Antibody (MIAP301) (10 μg/mL, 24 h) significantly inhibits epithelial cell migration and wound closure[1].
Anti-Mouse CD47/IAP Antibody (MIAP301) (0-4 h) inhibits the β1 integrin-FAK-SRc-p130Cas signaling pathway and reduces the level of p-SrcY 416, p-FAKY 397, p-FAKY Y861, p-p130Cas Y410 and β1 integrin[1].
Anti-Mouse CD47/IAP Antibody (MIAP301) (0-100 μg/mL, 1 h) effectively blocks CD47 and saturates its epitope in SM1 cells[2].
Anti-Mouse CD47/IAP Antibody (MIAP301) (25 μg/mL, 2-4 h) significantly enhances the phagocytic activity of macrophages towards SM1 cells[2].
Anti-Mouse CD47/IAP Antibody (MIAP301) (8 h) significantly restores the phagocytic ability of myeloid cells towards Escherichia coli[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Epithelial cells
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Concentration:10 μg/mL
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Incubation Time:24 h
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Result:Significant delayed in cell migration and wound closure.
In Vivo
Anti-Mouse CD47/IAP Antibody (MIAP301) (100 μg, intratumorally injection, once every three days, until the end of the study) significantly inhibits tumor growth and increases immune cell infiltration in the tumor microenvironment combined with Nexturastat A (HY-16699) in female C57BL/6 mice bearing SM1 or B16F10 tumors[2].
Anti-Mouse CD47/IAP Antibody (MIAP301) (2.5 mg/kg, i.v., single dose, 1 or 8 hours after cecal ligation and puncture (CLP) surgery) significantly reduces the number of bacteria in blood and organs and improves survival rates in Balb/c or C57/BL6 mice with a Klebsiella pneumonia-induced sepsis model[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57Bl/6J mice with a biopsy-based mucosal wound (8-12 weeks)[1]
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Dosage:400 μg
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Administration:Intraperitoneal injection (i.p.), single dose, 24 hours after the wound
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Result:Caused delayed wound closure.
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Animal Model:0.75×106 SM1 or B16F10 cells injected female C57BL/6J mice (4-6 weeks)[2]
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Dosage:100 μg, combined with Nexturastat A (20 mg/kg, i.p., once every other day)
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Administration:Intraperitoneal injection (i.p.), once every three days until the end of the study
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Result:Significantly inhibited tumor volume.
Significantly increased infiltration of macrophages and natural killer (NK) cells in SM1 models.
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Animal Model:Balb/c or C57/BL6 mice (8-10 weeks, 20-25 g) with a Klebsiella pneumonia-induced sepsis model[3]
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Dosage:2.5 mg/kg
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Administration:Intravenous injection (i.v.), single dose, 1 or 8 hours after CLP surgery
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Result:Significantly reduced bacterial load in blood and organs.
Improved survival rate (from 30% to 80%).
Reduced pathological damage to the lungs and kidneys.
Reduced the levels of inflammatory factors (TNF-α, IL-6, IL-1β, MCP-1).
Gene ID
Accession
Q61735-1
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
in vivo CD47 blockade; in vitro CD47 blockade; Immunofluorescence
Verified Bioactivity
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Flow cytometric analysis of 1X106 MC38 cells with Anti-Mouse CD47/IAP Antibody (MIAP301) (HY-P990131, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rat IgG H&L (HY-P89199) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rat IgG2a kappa (HY-P990679, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse CD47/IAP Antibody (MIAP301)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
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J Immunother Cancer
Spatial immune atlas of breast cancer brain metastasis reveals CD163+ macrophage reprogramming associated with immune escape. [Abstract]2026 Jun 24;14(6):e014421. PMID: 42342408 -
Cell Rep
THBS2-producing matrix CAFs promote colorectal cancer progression and link to poor prognosis via the CD47-MAPK axis. [Abstract]2025 Apr 11;44(4):115555. PMID: 40222008
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Reed M, et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo. Nat Commun. 2019 Nov 1;10(1):5004. [Content Brief]
[2]. Gracia-Hernandez M, et al. Targeting HDAC6 improves anti-CD47 immunotherapy. J Exp Clin Cancer Res. 2024 Feb 27;43(1):60. [Content Brief]
[3]. Feng Z, et al. CD47-amyloid-β-CD74 signaling triggers adaptive immunosuppression in sepsis. EMBO Rep. 2025 May;26(10):2683-2714. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CD47/IAP Antibody (MIAP301) Related Classifications
HY-P990131 Related Classifications
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