Anti-Mouse MAdCAM-1 Antibody (MECA-367)
Based on 1 Customer Validation
Anti-Mouse MAdCAM-1 Antibody (MECA-367) is an anti-mouse MAdCAM-1 IgG2a monoclonal antibody. Anti-Mouse MAdCAM-1 Antibody (MECA-367) significantly inhibits the migration of pig peripheral blood lymphocytes (PBL) by blocking the function of MAdCAM-1. Anti-Mouse MAdCAM-1 Antibody (MECA-367) selectively inhibits B cell migration to exacerbate inflammation. Anti-Mouse MAdCAM-1 Antibody (MECA-367) can be used for researches on inflammation, metabolic conditions and xenotransplantation such as colitis and diabetes.
For research use only. We do not sell to patients.
- Purity : 96.72%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
In Vivo
Anti-Mouse MAdCAM-1 Antibody (MECA-367) (100 μg, i.p., twice a week, for 8 weeks) can exacerbate colitis symptoms in IL-10-/- mice with a spontaneous chronic colitis model[2].
Anti-Mouse MAdCAM-1 Antibody (MECA-367) (500 μg, i.p., on day 0, 2 and 4) significantly inhibits diabetes in NOD scid mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:1×108 PBL cells injected SCID mice[1]
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Dosage:200 μg
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Administration:Intravenous injection (i.v.), single dose
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Result:Significantly reduced the migration of porcine PBL in the graft and blocked its entry into the graft.
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Animal Model:IL-10-/- mice with a spontaneous chronic colitis model[2]
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Dosage:100 μg
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Administration:Intraperitoneal injection (i.p.), twice a week, for 8 weeks
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Result:Led to a decrease in the number of B cells/antibody secreting cells (ASCs) in the colon.
Increased B cells in the spleen.
Significantly reduced fecal IgA/IgM and increased plasma IgA.
Reduced body weight and temperature, and increased colitis index.
Increased bacterial load, decreased alpha diversity, and altered beta diversity.
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Animal Model:1×107 BDC-2.5 T cells injected NOD scid mice (4-11 days)[3]
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Dosage:500 μg
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Administration:Intraperitoneal injection (i.p.), on day 0, 2 and 4
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Result:Significantly reduced the incidence rate of diabetes.
Accession
Q61826-1
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo MAdCAM-1 neutralization; Immunofluorescence
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse MAdCAM-1 Antibody (MECA-367)]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Whyte A, et al. Pig lymphocytes utilise mouse MAdCAM-1 to enter fetal gut xenografts in SCID mice. Cell Immunol. 1997 Nov 25;182(1):38-44. [Content Brief]
[2]. Tyler CJ, et al. Antibody secreting cells are critically dependent on integrin α4β7/MAdCAM-1 for intestinal recruitment and control of the microbiota during chronic colitis. Mucosal Immunol. 2022 Jan;15(1):109-119. [Content Brief]
[3]. Phillips JM, et al. MAdCAM-1 is needed for diabetes development mediated by the T cell clone, BDC-2.5. Immunology. 2005 Dec;116(4):525-31. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse MAdCAM-1 Antibody (MECA-367) Related Classifications
HY-P990197 Related Classifications
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