Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11)
Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) is rat-derived IgG2b κ type antibody inhibitor, targeting to PD-L1/B7-H1. Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) can block PD-L1/ B7-1 interactions and does not block PD-L1/PD-1 interactions. Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) can be used for the researches of cancer, infection, immunology and metabolic disease, such as MB49 tumor, heart graft and diabetes.
For research use only. We do not sell to patients.
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2b kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
PD-L1/B7-H1
In Vitro
Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) (3 h) does not affect T cell proliferation in CD4+ and CD8+ T cells from PD-1A transgenic mice[2].
Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) (50 μg/mL, 1 h) reduces replication of herpes simplex virus (HSV)-CD80 in dendritic cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) (0.5 mg at day 0 and 0.25 mg at days 2, 4, 6, 8 and 10, i.p.) induces diabetes in not obese diabetes of autoimmune diabetes mice[2].
Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) (0.5 mg at day 0 and 0.25 mg at days 2, 4, 6 and 8, i.p.) aggravates cardiac allograft vasculopathy and accelerates allograft rejection in bm12 hearts transferred PD1-deficient mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Not obese diabetes of autoimmune diabetes mice(6-7 weeks or 13 weeks)[1]
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Dosage:0.5 mg at day 0 and 0.25 mg at days 2, 4, 6, 8 and 10
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Administration:Intraperitoneally injection
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Result:Led to diabetes in 40% of 6-7-week mice over a 14 day period.
Led to diabetes in 80% of 13-week mice over a two-week period.
Showed marked infiltrates in pancreata.
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Animal Model:Bm12 hearts transferred PD1-deficient mice[3]
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Dosage:0.5 mg at day 0 and 0.25 mg at days 2, 4, 6 and 8
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Administration:Intraperitoneally injection
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Result:Showed median survival time of 10 days.
Increased the frequency of alloreactive T cells producing IFN-γ.
Enhanced Th1 and Th2 alloimmune responses.
Decreased regulatory T cell.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo blocking of PD-L1/CD80 (B7-1) interactions; in vitro blocking of PD-L1/CD80 (B7-1) interact
Chemical Information
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Molecular Weight 150 kDa
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SMILES
[Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Po Y. Ho, et al. PD-L1-directed ISACs target host immune cells to drive powerful antitumor immune
[2]. Paterson AM, et al. The programmed death-1 ligand 1:B7-1 pathway restrains diabetogenic effector T cells in vivo. J Immunol. 2011 Aug 1;187(3):1097-105. [Content Brief]
[3]. Yang J, et al. The novel costimulatory programmed death ligand 1/B7.1 pathway is functional in inhibiting alloimmune responses in vivo. J Immunol. 2011 Aug 1;187(3):1113-9. [Content Brief]
[4]. Mott KR, et al. Inclusion of CD80 in HSV targets the recombinant virus to PD-L1 on DCs and allows productive infection and robust immune responses. PLoS One. 2014 Jan 27;9(1):e87617. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) Related Classifications
HY-P990823 Related Classifications
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