Anti-Mouse TIM-4 Antibody (RMT4-54)
Anti-Mouse TIM-4 Antibody (RMT4-54) is a rat-derived IgG2a κ type antibody inhibitor, targeting to mouse TIM-4. Anti-Mouse TIM-4 Antibody (RMT4-54) reacts with mouse T cell immunoglobulin and mucin domain 4 (TIM-4) and blocks TIM-4 signaling. Anti-Mouse TIM-4 Antibody (RMT4-54) inhibits phagocytosis of apoptotic cells. Anti-Mouse TIM-4 Antibody (RMT4-54) can be used for the researches of immunology and metabolic disease.
For research use only. We do not sell to patients.
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
TIM-4
In Vitro
Anti-Mouse TIM-4 Antibody (RMT4-54) (20 μg/mL, 30 mins) abrogates binding of Tim-4/NRK cells to apoptotic cells[1].
Anti-Mouse TIM-4 Antibody (RMT4-54) (30 mins) inhibits apoptotic cells phagocytosis of peritoneal resident Mac1+ cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse TIM-4 Antibody (RMT4-54) (200 μg, i.p., 72 h and on the day prior to the overnight HFD) abrogated the release of high-density lipoproteins in high-fat diet (HFD) feeding mice models[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice[1]
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Dosage:200 μg/mouse
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Administration:Intraperitoneally injection, twice a week for 4 weeks
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Result:Significantly increased TUNEL+ cells.
Induced anti-dsDNA antibodies.
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Animal Model:High-fat diet (HFD) feeding mice models[2]
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Dosage:200 μg
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Administration:Intraperitoneally injection, 72 h and on the day prior to the overnight HFD
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Result:Specifically abrogated the release of high-density lipoproteins.
Increased CD36 expression on F4/80highTim4+ATMs.
Gene ID
Accession
Q6U7R4 / NP_848874.3
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vitro TIM-4 blocking; Immunofluorescence; Flow cytometry
Chemical Information
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Molecular Weight 150 kDa
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SMILES
[Anti-Mouse TIM-4 Antibody (RMT4-54)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Nakayama M, et al. Tim-3 mediates phagocytosis of apoptotic cells and cross-presentation. Blood. 2009 Apr 16;113(16):3821-30. [Content Brief]
[2]. Magalhaes MS, et al. Role of Tim4 in the regulation of ABCA1+ adipose tissue macrophages and post-prandial cholesterol levels. Nat Commun. 2021 Jul 21;12(1):4434. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse TIM-4 Antibody (RMT4-54) Related Classifications
HY-P990152 Related Classifications
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