Antibacterial agent 368

Antibacterial agent 368 (compound 4y) exhibits mild antibacterial activity against Enterococcus faecalis, Bacillus subtilis, Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli in vitro, producing inhibition zone diameters ranging from 6 to 7 mm at a concentration of 500 µg/mL^[1].
Antibacterial agent 368 inhibits the growth of Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli in vitro, with an MIC of 1024 µg/mL; it shows weak activity against Enterococcus faecalis (MIC > 1024 µg/mL); its MBC against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi is 1024 µg/mL, with an MBC/MIC ratio of 1^[1].
Antibacterial agent 368 exhibits dose-dependent in vitro antibacterial activity against Bacillus subtilis; when the concentration increases from 512 µg/mL to 2048 µg/mL, the diameter of the inhibition zone increases from 6 mm to 10 mm^[1].
Antibacterial agent 368 (at concentrations reduced from 1024 µg/mL to sub‑inhibitory levels) in combination with Ampicillin (HY‑B0522) exhibits synergistic antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa, with FICI values of 0.37 and 0.15, respectively^[1].
Antibacterial agent 368 (128-512 µg/mL; 48 h) acts as a moderate inhibitor of Pseudomonas aeruginosa biofilm formation, with an inhibition rate of 35% at 512 µg/mL, and its activity decreases at lower sub-inhibitory concentrations^[1].
Antibacterial agent 368 stably binds to human PelA protein, with favorable docking performance and excellent binding free energy values. It forms specific hydrogen bonds and maintains structural stability during 100 ns molecular dynamics simulation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.