Antibiotic DC 81
(Synonyms: DC 81)
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Antibiotic DC 81 (DC 81), an antitumor antibiotic produced by Streptomyces species, is a PBD (pyrrolo[2,1-c][1,4]benzodiazepine). Antibiotic DC 81 is potent inhibitor of nucleic acid synthesis. Antibiotic DC 81 can recognize and bind to specific sequences of DNA and form a labile covalent adduct.
For research use only. We do not sell to patients.
- Purity : 96.37%
- CAS No.: 81307-24-6
- Formula: C13H14N2O3
- Molecular Weight:246.26
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Biological Activity
Description
Antibiotic DC 81 (DC 81), an antitumor antibiotic produced by Streptomyces species, is a PBD (pyrrolo[2,1-c][1,4]benzodiazepine). Antibiotic DC 81 is potent inhibitor of nucleic acid synthesis. Antibiotic DC 81 can recognize and bind to specific sequences of DNA and form a labile covalent adduct[1][2].
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | GI50 |
0.14 μM
Compound: DC-81
|
Growth inhibition of human A2780 cells by SRB method
Growth inhibition of human A2780 cells by SRB method
|
[PMID: 18207392] |
| A2780 | GI50 |
0.14 μM
Compound: 3, DC-81
|
Growth inhibition of human A2780 cells after 48 hrs by SRB assay
Growth inhibition of human A2780 cells after 48 hrs by SRB assay
|
[PMID: 20031423] |
| A2780 | GI50 |
0.14 μM
Compound: 1, DC-81
|
Cytotoxicity against human A2780 cells after 48 hrs by SRB assay
Cytotoxicity against human A2780 cells after 48 hrs by SRB assay
|
[PMID: 20444601] |
| A2780 | GI50 |
0.14 μM
Compound: DC-81
|
Cytotoxicity against human A2780 cells after 48 hrs by SRB assay
Cytotoxicity against human A2780 cells after 48 hrs by SRB assay
|
[PMID: 20554354] |
| A2780 | GI50 |
0.14 μM
Compound: 4, DC-81
|
Anticancer activity against human A2780 cells after 48 hrs by SRB assay
Anticancer activity against human A2780 cells after 48 hrs by SRB assay
|
[PMID: 20557981] |
| A2780 | GI50 |
0.14 μM
Compound: 3, DC-81
|
Cytotoxicity against human A2780 cells after 48 hrs by SRB assay
Cytotoxicity against human A2780 cells after 48 hrs by SRB assay
|
[PMID: 20673627] |
| A2780 | GI50 |
0.14 μM
Compound: 1, DC-81
|
Cytotoxicity against human A2780 cells after 24 hrs by WST-1 assay
Cytotoxicity against human A2780 cells after 24 hrs by WST-1 assay
|
[PMID: 20732817] |
| A2780 | GI50 |
0.14 μM
Compound: 2, DC-81
|
Cytotoxicity against human A2780 cells by sulforhodamine B method
Cytotoxicity against human A2780 cells by sulforhodamine B method
|
[PMID: 21459581] |
| A2780 | GI50 |
0.14 μM
Compound: 1, DC-81
|
Anticancer activity against human A2780 cells by SRB method
Anticancer activity against human A2780 cells by SRB method
|
[PMID: 21676506] |
| A2780 | GI50 |
0.14 μM
Compound: 3, DC-81
|
Anticancer activity against human A2780 cells by SRB method
Anticancer activity against human A2780 cells by SRB method
|
[PMID: 22104151] |
| A2780 | GI50 |
0.14 μM
Compound: 1, DC-81
|
Growth inhibition of human A2780 cells
Growth inhibition of human A2780 cells
|
10.1039/C1MD00072A |
| A-431 | IC50 |
1.65 μM
Compound: I, DC-81
|
Anticancer activity against human A431 cells after 48 hrs by MTT assay
Anticancer activity against human A431 cells after 48 hrs by MTT assay
|
[PMID: 22209733] |
| A549 | GI50 |
0.16 μM
Compound: DC-81
|
Growth inhibition of human A549 cells by SRB method
Growth inhibition of human A549 cells by SRB method
|
[PMID: 18207392] |
| A549 | GI50 |
0.16 μM
Compound: 3, DC-81
|
Growth inhibition of human A549 cells after 48 hrs by SRB assay
Growth inhibition of human A549 cells after 48 hrs by SRB assay
|
[PMID: 20031423] |
| A549 | GI50 |
0.16 μM
Compound: 1, DC-81
|
Cytotoxicity against human A549 cells after 48 hrs by SRB assay
Cytotoxicity against human A549 cells after 48 hrs by SRB assay
|
[PMID: 20444601] |
| A549 | GI50 |
0.16 μM
Compound: DC-81
|
Cytotoxicity against human A549 cells after 48 hrs by SRB assay
Cytotoxicity against human A549 cells after 48 hrs by SRB assay
|
[PMID: 20554354] |
| A549 | GI50 |
0.16 μM
Compound: 3, DC-81
|
Cytotoxicity against human A549 cells after 48 hrs by SRB assay
Cytotoxicity against human A549 cells after 48 hrs by SRB assay
|
[PMID: 20673627] |
| A549 | GI50 |
0.16 μM
Compound: 1, DC-81
|
Cytotoxicity against human A549 cells after 24 hrs by WST-1 assay
Cytotoxicity against human A549 cells after 24 hrs by WST-1 assay
|
[PMID: 20732817] |
| A549 | GI50 |
0.16 μM
Compound: 2, DC-81
|
Cytotoxicity against human A549 cells by sulforhodamine B method
Cytotoxicity against human A549 cells by sulforhodamine B method
|
[PMID: 21459581] |
| A549 | GI50 |
0.16 μM
Compound: 1, DC-81
|
Anticancer activity against human A549 cells by SRB method
Anticancer activity against human A549 cells by SRB method
|
[PMID: 21676506] |
| A549 | GI50 |
0.16 μM
Compound: 3, DC-81
|
Anticancer activity against human A549 cells by SRB method
Anticancer activity against human A549 cells by SRB method
|
[PMID: 22104151] |
| A549 | IC50 |
1.11 μM
Compound: I, DC-81
|
Anticancer activity against human A549 cells after 48 hrs by MTT assay
Anticancer activity against human A549 cells after 48 hrs by MTT assay
|
[PMID: 22209733] |
| A549 | GI50 |
0.16 μM
Compound: 1, DC-81
|
Growth inhibition of human A549 cells
Growth inhibition of human A549 cells
|
10.1039/C1MD00072A |
| CH1 | IC50 |
0.1 μM
Compound: 5
|
Tested for cytotoxicity against CH1 human ovarian cells
Tested for cytotoxicity against CH1 human ovarian cells
|
[PMID: 10354403] |
| CH1 | IC50 |
0.1 μM
Compound: 3
|
In vitro cytotoxicity against ovarian carcinoma cell line CH1.
In vitro cytotoxicity against ovarian carcinoma cell line CH1.
|
[PMID: 10514273] |
| CHO-AA8 | IC50 |
2 μM
Compound: 31 (DC-81)
|
In vitro cellular toxicity against chinese hamster AA8 cell line after 4 hr of compound exposure
In vitro cellular toxicity against chinese hamster AA8 cell line after 4 hr of compound exposure
|
[PMID: 12747786] |
| COLO 205 | GI50 |
0.11 μM
Compound: DC-81
|
Growth inhibition of human Colo205 cells by SRB method
Growth inhibition of human Colo205 cells by SRB method
|
[PMID: 18207392] |
| COLO 205 | GI50 |
0.11 μM
Compound: 3, DC-81
|
Growth inhibition of human COLO205 cells after 48 hrs by SRB assay
Growth inhibition of human COLO205 cells after 48 hrs by SRB assay
|
[PMID: 20031423] |
| COLO 205 | GI50 |
0.11 μM
Compound: 1, DC-81
|
Cytotoxicity against human COLO205 cells after 48 hrs by SRB assay
Cytotoxicity against human COLO205 cells after 48 hrs by SRB assay
|
[PMID: 20444601] |
| COLO 205 | GI50 |
0.11 μM
Compound: DC-81
|
Cytotoxicity against human COLO205 cells after 48 hrs by SRB assay
Cytotoxicity against human COLO205 cells after 48 hrs by SRB assay
|
[PMID: 20554354] |
| COLO 205 | GI50 |
0.11 μM
Compound: 3, DC-81
|
Cytotoxicity against human COLO205 cells after 48 hrs by SRB assay
Cytotoxicity against human COLO205 cells after 48 hrs by SRB assay
|
[PMID: 20673627] |
| COLO 205 | GI50 |
0.11 μM
Compound: 1, DC-81
|
Cytotoxicity against human COLO205 cells after 24 hrs by WST-1 assay
Cytotoxicity against human COLO205 cells after 24 hrs by WST-1 assay
|
[PMID: 20732817] |
| COLO 205 | GI50 |
0.11 μM
Compound: 2, DC-81
|
Cytotoxicity against human COLO205 cells by sulforhodamine B method
Cytotoxicity against human COLO205 cells by sulforhodamine B method
|
[PMID: 21459581] |
| COLO 205 | GI50 |
0.11 μM
Compound: 1, DC-81
|
Anticancer activity against human COLO205 cells by SRB method
Anticancer activity against human COLO205 cells by SRB method
|
[PMID: 21676506] |
| COLO 205 | GI50 |
0.11 μM
Compound: 3, DC-81
|
Anticancer activity against human COLO205 cells by SRB method
Anticancer activity against human COLO205 cells by SRB method
|
[PMID: 22104151] |
| COLO 205 | IC50 |
0.86 μM
Compound: I, DC-81
|
Anticancer activity against human COLO205 cells after 48 hrs by MTT assay
Anticancer activity against human COLO205 cells after 48 hrs by MTT assay
|
[PMID: 22209733] |
| COLO 205 | GI50 |
0.11 μM
Compound: 1, DC-81
|
Growth inhibition of human COLO205 cells
Growth inhibition of human COLO205 cells
|
10.1039/C1MD00072A |
| EMT6 | IC50 |
1100 nM
Compound: 31 (DC-81)
|
In vitro cellular toxicity against murine mammary carcinoma EMT6 cell line after 4 hr of compound exposure
In vitro cellular toxicity against murine mammary carcinoma EMT6 cell line after 4 hr of compound exposure
|
[PMID: 12747786] |
| HOP-62 | GI50 |
0.15 μM
Compound: 3, DC-81
|
Growth inhibition of human HOP62 cells after 48 hrs by SRB assay
Growth inhibition of human HOP62 cells after 48 hrs by SRB assay
|
[PMID: 20031423] |
| HOP-62 | GI50 |
0.15 μM
Compound: 1, DC-81
|
Cytotoxicity against human HOP62 cells after 48 hrs by SRB assay
Cytotoxicity against human HOP62 cells after 48 hrs by SRB assay
|
[PMID: 20444601] |
| HOP-62 | GI50 |
0.15 μM
Compound: DC-81
|
Cytotoxicity against human HOP62 cells after 48 hrs by SRB assay
Cytotoxicity against human HOP62 cells after 48 hrs by SRB assay
|
[PMID: 20554354] |
| HOP-62 | GI50 |
0.15 μM
Compound: 3, DC-81
|
Cytotoxicity against human HOP62 cells after 48 hrs by SRB assay
Cytotoxicity against human HOP62 cells after 48 hrs by SRB assay
|
[PMID: 20673627] |
| HOP-62 | GI50 |
0.15 μM
Compound: 1, DC-81
|
Cytotoxicity against human HOP62 cells after 24 hrs by WST-1 assay
Cytotoxicity against human HOP62 cells after 24 hrs by WST-1 assay
|
[PMID: 20732817] |
| HOP-62 | GI50 |
0.15 μM
Compound: 2, DC-81
|
Cytotoxicity against human HOP62 cells by sulforhodamine B method
Cytotoxicity against human HOP62 cells by sulforhodamine B method
|
[PMID: 21459581] |
| HOP-62 | GI50 |
0.15 μM
Compound: 1, DC-81
|
Anticancer activity against human HOP62 cells by SRB method
Anticancer activity against human HOP62 cells by SRB method
|
[PMID: 21676506] |
| HOP-62 | GI50 |
0.15 μM
Compound: 1, DC-81
|
Growth inhibition of human HOP62 cells
Growth inhibition of human HOP62 cells
|
10.1039/C1MD00072A |
| HT-29 | IC50 |
30.19 μM
Compound: 16b, DC-81
|
Cytotoxicity against human HT29 cells by MTT assay
Cytotoxicity against human HT29 cells by MTT assay
|
[PMID: 18538566] |
| HT-29 | IC50 |
30.19 μmol
Compound: 16b, DC-81
|
Cytotoxicity against human HT29 cells by MTT assay
Cytotoxicity against human HT29 cells by MTT assay
|
[PMID: 18538566] |
| Jurkat | IC50 |
2.2 μM
Compound: 9, DC-81
|
Antiproliferative activity against human Jurkat cells
Antiproliferative activity against human Jurkat cells
|
[PMID: 17081759] |
| K562 | IC50 |
1 μM
Compound: 9, DC-81
|
Antiproliferative activity against human K562 cells
Antiproliferative activity against human K562 cells
|
[PMID: 17081759] |
| KB | GI50 |
0.17 μM
Compound: DC-81
|
Growth inhibition of human KB cells by SRB method
Growth inhibition of human KB cells by SRB method
|
[PMID: 18207392] |
| KB | GI50 |
0.17 μM
Compound: 3, DC-81
|
Growth inhibition of human KB cells after 48 hrs by SRB assay
Growth inhibition of human KB cells after 48 hrs by SRB assay
|
[PMID: 20031423] |
| KB | GI50 |
0.17 μM
Compound: 1, DC-81
|
Cytotoxicity against human KB cells after 48 hrs by SRB assay
Cytotoxicity against human KB cells after 48 hrs by SRB assay
|
[PMID: 20444601] |
| KB | GI50 |
0.17 μM
Compound: DC-81
|
Cytotoxicity against human KB cells after 48 hrs by SRB assay
Cytotoxicity against human KB cells after 48 hrs by SRB assay
|
[PMID: 20554354] |
| KB | GI50 |
0.17 μM
Compound: 4, DC-81
|
Anticancer activity against human KB cells after 48 hrs by SRB assay
Anticancer activity against human KB cells after 48 hrs by SRB assay
|
[PMID: 20557981] |
| KB | GI50 |
0.17 μM
Compound: 3, DC-81
|
Cytotoxicity against human KB cells after 48 hrs by SRB assay
Cytotoxicity against human KB cells after 48 hrs by SRB assay
|
[PMID: 20673627] |
| KB | GI50 |
0.17 μM
Compound: 1, DC-81
|
Cytotoxicity against human KB cells after 24 hrs by WST-1 assay
Cytotoxicity against human KB cells after 24 hrs by WST-1 assay
|
[PMID: 20732817] |
| KB | GI50 |
0.17 μM
Compound: 2, DC-81
|
Cytotoxicity against human KB cells by sulforhodamine B method
Cytotoxicity against human KB cells by sulforhodamine B method
|
[PMID: 21459581] |
| KB | GI50 |
0.17 μM
Compound: 1, DC-81
|
Anticancer activity against human KB cells by SRB method
Anticancer activity against human KB cells by SRB method
|
[PMID: 21676506] |
| KB | GI50 |
0.17 μM
Compound: 3, DC-81
|
Anticancer activity against human KB cells by SRB method
Anticancer activity against human KB cells by SRB method
|
[PMID: 22104151] |
| KB | GI50 |
0.17 μM
Compound: 1, DC-81
|
Growth inhibition of human KB cells
Growth inhibition of human KB cells
|
10.1039/C1MD00072A |
| L1210 | IC50 |
0.38 μM
Compound: 5
|
Tested for cytotoxicity against L1210 Leukemia cell
Tested for cytotoxicity against L1210 Leukemia cell
|
[PMID: 10354403] |
| L1210 | IC50 |
0.38 μM
Compound: DC-81
|
In vitro cytotoxicity was evaluated in L1210 cell line
In vitro cytotoxicity was evaluated in L1210 cell line
|
[PMID: 14505647] |
| L1210 | IC50 |
0.38 μM
Compound: DC-81
|
In vitro cytotoxicity against leukemia L1210 cell line
In vitro cytotoxicity against leukemia L1210 cell line
|
[PMID: 14698184] |
| L1210 | IC50 |
0.38 μM
Compound: DC-81
|
Compound was tested for inhibitory activity against L1210 cell line
Compound was tested for inhibitory activity against L1210 cell line
|
[PMID: 15225736] |
| L1210 | IC50 |
0.38 μM
Compound: DC-81
|
In vitro cytotoxicity against mouse leukemia L1210 cell lines
In vitro cytotoxicity against mouse leukemia L1210 cell lines
|
[PMID: 15341949] |
| L1210 | IC50 |
0.38 μM
Compound: DC-81, 2
|
Cytotoxicity against mouse L1210 cells
Cytotoxicity against mouse L1210 cells
|
[PMID: 18378445] |
| L1210 | IC50 |
0.38 μM
Compound: DC-81
|
Tested in vitro for inhibition after 48 hr exposure against murine leukemia cell line L1210
Tested in vitro for inhibition after 48 hr exposure against murine leukemia cell line L1210
|
[PMID: 7996544] |
| MCF7 | GI50 |
0.17 μM
Compound: DC-81
|
Growth inhibition of human MCF7 cells by SRB method
Growth inhibition of human MCF7 cells by SRB method
|
[PMID: 18207392] |
| MCF7 | GI50 |
0.17 μM
Compound: 3, DC-81
|
Growth inhibition of human MCF7 cells after 48 hrs by SRB assay
Growth inhibition of human MCF7 cells after 48 hrs by SRB assay
|
[PMID: 20031423] |
| MCF7 | GI50 |
0.17 μM
Compound: 1, DC-81
|
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
|
[PMID: 20444601] |
| MCF7 | GI50 |
0.17 μM
Compound: DC-81
|
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
|
[PMID: 20554354] |
| MCF7 | GI50 |
0.17 μM
Compound: 3, DC-81
|
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
|
[PMID: 20673627] |
| MCF7 | GI50 |
0.17 μM
Compound: 1, DC-81
|
Cytotoxicity against human MCF7 cells after 24 hrs by WST-1 assay
Cytotoxicity against human MCF7 cells after 24 hrs by WST-1 assay
|
[PMID: 20732817] |
| MCF7 | GI50 |
0.17 μM
Compound: 2, DC-81
|
Cytotoxicity against human MCF7 cells by sulforhodamine B method
Cytotoxicity against human MCF7 cells by sulforhodamine B method
|
[PMID: 21459581] |
| MCF7 | GI50 |
0.17 μM
Compound: 1, DC-81
|
Anticancer activity against human MCF7 cells by SRB method
Anticancer activity against human MCF7 cells by SRB method
|
[PMID: 21676506] |
| MCF7 | GI50 |
0.17 μM
Compound: 3, DC-81
|
Anticancer activity against human MCF7 cells by SRB method
Anticancer activity against human MCF7 cells by SRB method
|
[PMID: 22104151] |
| MCF7 | GI50 |
0.17 μM
Compound: 1, DC-81
|
Growth inhibition of human MCF7 cells
Growth inhibition of human MCF7 cells
|
10.1039/C1MD00072A |
| MCF7 | IC50 |
5 μM
Compound: 1, DC-81
|
Cytotoxicity against human MCF7 cells assessed as reduction cell viability after 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as reduction cell viability after 24 hrs by MTT assay
|
10.1039/C2MD20219K |
| PC-3 | GI50 |
0.2 μM
Compound: DC-81
|
Growth inhibition of human PC3 cells by SRB method
Growth inhibition of human PC3 cells by SRB method
|
[PMID: 18207392] |
| PC-3 | GI50 |
0.2 μM
Compound: 3, DC-81
|
Growth inhibition of human PC3 cells after 48 hrs by SRB assay
Growth inhibition of human PC3 cells after 48 hrs by SRB assay
|
[PMID: 20031423] |
| PC-3 | GI50 |
0.2 μM
Compound: 1, DC-81
|
Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
|
[PMID: 20444601] |
| PC-3 | GI50 |
0.2 μM
Compound: DC-81
|
Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
|
[PMID: 20554354] |
| PC-3 | GI50 |
0.2 μM
Compound: 3, DC-81
|
Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
|
[PMID: 20673627] |
| PC-3 | GI50 |
0.2 μM
Compound: 1, DC-81
|
Cytotoxicity against human PC3 cells after 24 hrs by WST-1 assay
Cytotoxicity against human PC3 cells after 24 hrs by WST-1 assay
|
[PMID: 20732817] |
| PC-3 | GI50 |
0.2 μM
Compound: 2, DC-81
|
Cytotoxicity against human PC3 cells by sulforhodamine B method
Cytotoxicity against human PC3 cells by sulforhodamine B method
|
[PMID: 21459581] |
| PC-3 | GI50 |
0.2 μM
Compound: 1, DC-81
|
Anticancer activity against human PC3 cells by SRB method
Anticancer activity against human PC3 cells by SRB method
|
[PMID: 21676506] |
| PC-3 | IC50 |
1.19 μM
Compound: I, DC-81
|
Anticancer activity against human PC3 cells after 48 hrs by MTT assay
Anticancer activity against human PC3 cells after 48 hrs by MTT assay
|
[PMID: 22209733] |
| PC-3 | GI50 |
0.2 μM
Compound: 1, DC-81
|
Growth inhibition of human PC3 cells
Growth inhibition of human PC3 cells
|
10.1039/C1MD00072A |
| PC-6 | IC50 |
0.33 μM
Compound: DC-81
|
In vitro cytotoxicity was evaluated in PC6 cell line
In vitro cytotoxicity was evaluated in PC6 cell line
|
[PMID: 14505647] |
| PC-6 | IC50 |
0.33 μM
Compound: DC-81
|
In vitro cytotoxicity against leukemia PC6 cell line
In vitro cytotoxicity against leukemia PC6 cell line
|
[PMID: 14698184] |
| PC-6 | IC50 |
0.33 μM
Compound: DC-81
|
Compound was tested for inhibitory activity against PC6 cell line
Compound was tested for inhibitory activity against PC6 cell line
|
[PMID: 15225736] |
| PC-6 | IC50 |
0.33 μM
Compound: DC-81
|
In vitro cytotoxicity against human lung cancer PC-6 cells lines
In vitro cytotoxicity against human lung cancer PC-6 cells lines
|
[PMID: 15341949] |
| PC-6 | IC50 |
0.33 μM
Compound: DC-81, 2
|
Cytotoxicity against human PC6 cells
Cytotoxicity against human PC6 cells
|
[PMID: 18378445] |
| SiHa | GI50 |
0.17 μM
Compound: DC-81
|
Growth inhibition of human SiHa cells by SRB method
Growth inhibition of human SiHa cells by SRB method
|
[PMID: 18207392] |
| SiHa | GI50 |
0.17 μM
Compound: 3, DC-81
|
Growth inhibition of human SiHa cells after 48 hrs by SRB assay
Growth inhibition of human SiHa cells after 48 hrs by SRB assay
|
[PMID: 20031423] |
| SiHa | GI50 |
0.17 μM
Compound: 1, DC-81
|
Cytotoxicity against human SiHa cells after 48 hrs by SRB assay
Cytotoxicity against human SiHa cells after 48 hrs by SRB assay
|
[PMID: 20444601] |
| SiHa | GI50 |
0.17 μM
Compound: DC-81
|
Cytotoxicity against human SiHa cells after 48 hrs by SRB assay
Cytotoxicity against human SiHa cells after 48 hrs by SRB assay
|
[PMID: 20554354] |
| SiHa | GI50 |
0.17 μM
Compound: 4, DC-81
|
Anticancer activity against human SiHa cells after 48 hrs by SRB assay
Anticancer activity against human SiHa cells after 48 hrs by SRB assay
|
[PMID: 20557981] |
| SiHa | GI50 |
0.17 μM
Compound: 3, DC-81
|
Cytotoxicity against human SiHa cells after 48 hrs by SRB assay
Cytotoxicity against human SiHa cells after 48 hrs by SRB assay
|
[PMID: 20673627] |
| SiHa | GI50 |
0.17 μM
Compound: 1, DC-81
|
Cytotoxicity against human SiHa cells after 24 hrs by WST-1 assay
Cytotoxicity against human SiHa cells after 24 hrs by WST-1 assay
|
[PMID: 20732817] |
| SiHa | GI50 |
0.17 μM
Compound: 2, DC-81
|
Cytotoxicity against human SiHa cells by sulforhodamine B method
Cytotoxicity against human SiHa cells by sulforhodamine B method
|
[PMID: 21459581] |
| SiHa | GI50 |
0.17 μM
Compound: 1, DC-81
|
Anticancer activity against human SiHa cells by SRB method
Anticancer activity against human SiHa cells by SRB method
|
[PMID: 21676506] |
| SiHa | GI50 |
0.16 μM
Compound: 3, DC-81
|
Anticancer activity against human SiHa cells by SRB method
Anticancer activity against human SiHa cells by SRB method
|
[PMID: 22104151] |
| SiHa | GI50 |
0.17 μM
Compound: 1, DC-81
|
Growth inhibition of human SiHa cells
Growth inhibition of human SiHa cells
|
10.1039/C1MD00072A |
| SK-OV-3 | IC50 |
1900 nM
Compound: 31 (DC-81)
|
In vitro cellular toxicity against human ovarian carcinoma SKOV3 cell line after 4 hr of compound exposure
In vitro cellular toxicity against human ovarian carcinoma SKOV3 cell line after 4 hr of compound exposure
|
[PMID: 12747786] |
| UV4 | IC50 |
1500 nM
Compound: 31 (DC-81)
|
In vitro cellular toxicity against chinese hamster UV4 cell line after 4 hr of compound exposure
In vitro cellular toxicity against chinese hamster UV4 cell line after 4 hr of compound exposure
|
[PMID: 12747786] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: DC-81
|
Growth inhibition of human Zr-75-1 cells by SRB method
Growth inhibition of human Zr-75-1 cells by SRB method
|
[PMID: 18207392] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: 3, DC-81
|
Growth inhibition of human ZR-75-1 cells after 48 hrs by SRB assay
Growth inhibition of human ZR-75-1 cells after 48 hrs by SRB assay
|
[PMID: 20031423] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: 1, DC-81
|
Cytotoxicity against human ZR-75-1 cells after 48 hrs by SRB assay
Cytotoxicity against human ZR-75-1 cells after 48 hrs by SRB assay
|
[PMID: 20444601] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: DC-81
|
Cytotoxicity against human ZR-75-1 cells after 48 hrs by SRB assay
Cytotoxicity against human ZR-75-1 cells after 48 hrs by SRB assay
|
[PMID: 20554354] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: 4, DC-81
|
Anticancer activity against human ZR-75-1 cells after 48 hrs by SRB assay
Anticancer activity against human ZR-75-1 cells after 48 hrs by SRB assay
|
[PMID: 20557981] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: 3, DC-81
|
Cytotoxicity against human ZR-75-1 cells after 48 hrs by SRB assay
Cytotoxicity against human ZR-75-1 cells after 48 hrs by SRB assay
|
[PMID: 20673627] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: 1, DC-81
|
Cytotoxicity against human ZR-75-1 cells after 24 hrs by WST-1 assay
Cytotoxicity against human ZR-75-1 cells after 24 hrs by WST-1 assay
|
[PMID: 20732817] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: 2, DC-81
|
Cytotoxicity against human ZR-75-1 cells by sulforhodamine B method
Cytotoxicity against human ZR-75-1 cells by sulforhodamine B method
|
[PMID: 21459581] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: 1, DC-81
|
Anticancer activity against human ZR-75-1 cells by SRB method
Anticancer activity against human ZR-75-1 cells by SRB method
|
[PMID: 21676506] |
| ZR-75-1 | GI50 |
2.37 μM
Compound: 3, DC-81
|
Anticancer activity against human ZR-75-1 cells by SRB method
Anticancer activity against human ZR-75-1 cells by SRB method
|
[PMID: 22104151] |
| ZR-75-1 | GI50 |
2.34 μM
Compound: 1, DC-81
|
Growth inhibition of human ZR-75-1 cells
Growth inhibition of human ZR-75-1 cells
|
10.1039/C1MD00072A |
In Vitro
Antibiotic DC 81 shows cytotoxicity against human melanoma cell lines B16, A375, A2058, and RPMI7951, with IC50 values of 4.4 μM, 18.5 μM, 31.0 μM, and 41.5 μM, respectively[1][2].
Antibiotic DC 81 exhibits its biological activity by covalently binding to the N2 of guanine in the minor groove of DNA, via the electrophilic carbinolamine functionality at N10-C11[1].
Antibiotic DC 81 (4 μM, 24 h) induces mitochondria dependent apoptosis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Apoptosis Analysis[2]
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Cell Line:B16 melanoma cells
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Concentration:4 μM
-
Incubation Time:24 h
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Result:Induced mitochondria dependent apoptosis.
In Vivo
Antibiotic DC 81 (0-10 mg/kg, i.p., at day 4, 7, 10, 13 after tumor cell injection) decreases the tumor burden in tumor-bearing mice, but the Antibiotic DC 81 at 10 mg/kg impairs cardiac muscle enzyme and liver function significantly[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Female C57BL/6 mice (8-12 weeks old, B16 cells were injected into the tail veins of mice)[2]
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Dosage:0.1, 1, 10 mg/kg
-
Administration:i.p., at day 4, 7, 10, 13 after tumor cell injection
-
Result:Substantially decreased the tumor burden by 20% at 1 mg/kg. DC-81 at 10 mg/kg induced an 8-10-fold increase of GPT and a 6-8-fold increase in CPK, which indicated severe impaired liver function and muscle damage. Did not impair significant renal function as demonstrated by serum creatinine.
Chemical Information
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CAS No. 81307-24-6
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Appearance Solid
-
Molecular Weight 246.26
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Formula C13H14N2O3
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Color White to off-white
-
SMILES
O=C1C2=CC(OC)=C(O)C=C2N=C[C@@]3([H])N1CCC3
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Synonyms
DC 81
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Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 20 mg/mL (81.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
View the Complete Stock Solution Preparation Table
Preparing Stock Solutions
View the Complete Stock Solution Preparation Table
Concentration
Solvent
Mass
1 mg
5 mg
10 mg
Concentration
Solvent
Mass
1 mM
4.0607 mL
20.3037 mL
40.6075 mL
5 mM
0.8121 mL
4.0607 mL
8.1215 mL
10 mM
0.4061 mL
2.0304 mL
4.0607 mL
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Molarity Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
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Dilution Calculator
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
Select Batch:
Purity:
99.98%
Assay:
99.98%
ee.:
99.98%
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hu WP, et al. Biological evaluation of an antibiotic DC-81-indole conjugate agent in human melanoma cell lines. Kaohsiung J Med Sci. 2003 Jan;19(1):6-12. [Content Brief]
[2]. Lee CH, et al. Pyrrolo[2,1-c][1,4]benzodiazepine and indole conjugate (IN6CPBD) has better efficacy and superior safety than the mother compound DC-81 in suppressing the growth of established melanoma in vivo. Chem Biol Interact. 2009 Aug 14;180(3):360-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0607 mL | 20.3037 mL | 40.6075 mL | 101.5187 mL |
| 5 mM | 0.8121 mL | 4.0607 mL | 8.1215 mL | 20.3037 mL | |
| 10 mM | 0.4061 mL | 2.0304 mL | 4.0607 mL | 10.1519 mL | |
| 15 mM | 0.2707 mL | 1.3536 mL | 2.7072 mL | 6.7679 mL | |
| 20 mM | 0.2030 mL | 1.0152 mL | 2.0304 mL | 5.0759 mL | |
| 25 mM | 0.1624 mL | 0.8121 mL | 1.6243 mL | 4.0607 mL | |
| 30 mM | 0.1354 mL | 0.6768 mL | 1.3536 mL | 3.3840 mL | |
| 40 mM | 0.1015 mL | 0.5076 mL | 1.0152 mL | 2.5380 mL | |
| 50 mM | 0.0812 mL | 0.4061 mL | 0.8121 mL | 2.0304 mL | |
| 60 mM | 0.0677 mL | 0.3384 mL | 0.6768 mL | 1.6920 mL | |
| 80 mM | 0.0508 mL | 0.2538 mL | 0.5076 mL | 1.2690 mL |
Antibiotic DC 81 Related Classifications
HY-107767 Related Classifications
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