BCL2L10 Antibody (YA4509)

(Synonyms: Boo; Diva; BCL-B; bcl2-L-10)
Customer Review

Based on 1 Customer Validation

BCL2L10 Antibody (YA4509) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BCL2L10.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

BCL2L10 Antibody (YA4509) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BCL2L10.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 22 kDa
Immunogen

Purified recombinant fragment of human BCL2L10 (AA: 31-186) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718987

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BCL2L10 Antibody (YA4509)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using BCL2L10 Antibody (HY-P84812, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BCL2L10 Antibody (YA4509)
    Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using BCL2L10 Antibody (HY-P84812, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BCL2L10 Antibody (YA4509)
    Immunohistochemical analysis of paraffin-embedded human glioma tissue using BCL2L10 Antibody (HY-P84812, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BCL2L10 Antibody (YA4509)
    Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using BCL2L10 Antibody (HY-P84812, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BCL2L10 Antibody (YA4509)
    Immunohistochemical analysis of paraffin-embedded human melanoma tissue using BCL2L10 Antibody (HY-P84812, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BCL2L10 Antibody (YA4509)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using BCL2L10 Antibody (HY-P84812, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BCL2L10 Antibody (YA4509)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using BCL2L10 Antibody (HY-P84812, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BCL2L10 Antibody (YA4509)
    Immunofluorescence analysis of GC-7901 cells labeling BCL2L10 antibody (HY-P84812) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

  • Function

    BCL2L10 promotes cell survival by suppressing apoptosis induced by BAX but not BAK. Increases binding of AHCYL1/IRBIT to ITPR1. Reduces ITPR1-mediated calcium release from the endoplasmic reticulum cooperatively with AHCYL1/IRBIT under normal cellular conditions. Under apoptotic stress conditions, dissociates from ITPR1 and is displaced from mitochondria-associated endoplasmic reticulum membranes, leading to increased Ca(2+) transfer to mitochondria which promotes apoptosis. Required for the correct formation of the microtubule organizing center during oocyte cell division, potentially via regulation of protein abundance and localization of other microtubule organizing center components such as AURKA and TPX2[1][2][3].

  • Subcellular Localization

    Mitochondrion; Nucleus membrane; Endoplasmic reticulum; Cytoplasm, cytoskeleton, spindle

  • Expression


    Tissue_specificity:Widely expressed in adult tissues. Preferentially expressed in lung, liver and kidney

  • Subunit

    Interacts with BAX (PubMed:11278245, PubMed:23235460). Interacts with BCL2 and BCL2L1/BCLX (PubMed:11278245, PubMed:11593390). Interacts with APAF1 (By similarity).

  • SwissProt ID

    Q9HD36

  • Gene ID
  • Synonyms

    Boo; Diva; BCL-B; bcl2-L-10

BCL2L10 Antibody (YA4509) Related Classifications

MOQ
Minimum order quantity
100 mg

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