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  5. Brk/PTK6 Antibody (YA1203)

Brk/PTK6 Antibody (YA1203)

Cat. No.: HY-P81458
User Guide for Antibodies Technical Support

Brk/PTK6 Antibody (YA1203) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Brk/PTK6.

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사이즈 가격 재고 수량
20 μL 견적 받기 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μL 견적 받기 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μL 견적 받기 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
250 μL   견적 받기  
Synthetic products have potential research and development risk.
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • 제품 설명

제품 설명

Brk/PTK6 Antibody (YA1203) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Brk/PTK6.
Host

Rabbit
Clonality

Recombinant, Monoclonal
분자량
Predicted band size: 52 kDa;
Observed band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human Brk
Application &
Dilution Ratio
Application Dilution Ratio
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-1:200
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.283.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Background
Function：Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Phosphorylates the GTPase-activating protein ARAP1 following EGF stimulation which enhances EGFR signaling by delaying EGFR down-regulation (PubMed:20554524). Also associates with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways; Inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins
Subcellular Localization：Cytoplasm; Nucleus; Cell projection, ruffle; Membrane
Expression：
Tissue_specificity:Epithelial-specific expression. Extremely high expression levels in the colon, high levels in the small intestine and prostate, and low levels in some fetal tissues. Not expressed in breast or ovarian tissues, but expressed in a high proportion of breast and ovarian cancers. Overexpressed in some metastatic melanomas, lymphomas, colon cancers, squamous cell carcinomas, and prostate cancers. Also found in melanocytes. Not expressed in the heart, brain, placenta, lungs, liver, skeletal muscle, kidneys, and pancreas. Isomer 2 is present in prostatic epithelial cell lines derived from normal prostate and prostate adenocarcinoma, as well as in various other cell lines.
Isoforms & Post-Translational Modification：Q13882 has 2 isomers: Q13882-1: 51834 Da (predicted); Q13882-2: 14465 Da (predicted).
Autophosphorylated. Autophosphorylation of Tyr-342 leads to an increase of kinase activity. Tyr-447 binds to the SH2 domain when phosphorylated and negatively regulates kinase activity
Subunit：Interacts (via SH3 and SH2 domains) with KHDRBS1. Interacts (via SH3 and SH2 domains) with phosphorylated IRS4. Interacts with ADAM15. Interacts (via SH3 domain) with SFPQ. Interacts with EGFR and ERBB2.
Database

Entrez Gene: 5753 Human

SwissProt: Q13882 Human

OMIM: 602004 Human

Research Field

Signal Transduction
Synonyms
PTK6; BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK
각종 서류
User Guide for Antibodies (1077 KB)

Brk/PTK6 Antibody (YA1203) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Brk/PTK6 Antibody (YA1203)
Cat. No.:
HY-P81458
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