CLEC12A Antibody (YA4684)

Cat. No.: HY-P84992: Ficha de datos User Guide for Antibodies
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CLEC12A Antibody (YA4684) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CLEC12A.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

CLEC12A Antibody (YA4684) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CLEC12A.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human

SwissProt ID

Q5QGZ9

Gene ID

160364 [NCBI]

Immunogen

Purified recombinant Human CD371

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-100000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in Phosphate-buffered solution.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Myeloid inhibitory C-type lectin receptor that acts as a negative regulator of myeloid cell activation (PubMed:14739280, PubMed:15238421, PubMed:16239426, PubMed:34234773, PubMed:38367667, PubMed:38386511, PubMed:39143217). Myeloid cell inhibition is required to limit proinflammatory pathways and protect against excessive inflammation (By similarity). Specifically recognizes and binds various structures, such as neutrophil extracellular traps (NETs) or monosodium urate crystals (PubMed:38367667, PubMed:38386511, PubMed:39143217). Also acts as a pattern-recognition receptor for pathogen-associated molecules, such as plasmodium hemozoin or mycobacterial micolic acid (PubMed:31269448, PubMed:36542980). Ligand-binding induces phosphorylation of its ITIM motif, followed by recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11, which counteract tyrosine-protein kinase SYK, thereby preventing myeloid cell activation (PubMed:14739280, PubMed:16239426, PubMed:34234773). Acts as a pattern-recognition receptor for NETs in neutrophils: specifically recognizes DNA in NETs, leading to inhibit neutrophil activation and limit further NET formation (PubMed:39143217). This regulation is essential for controlling key neutrophil responses and limit NET-mediated inflammatory conditions (By similarity). Also recognizes dead cells by acting as a receptor for monosodium urate crystals, leading to down-regulate neutrophil activation (PubMed:38367667, PubMed:38386511). Binding to monosodium urate crystals also promotes the type I interferon response (By similarity). Acts as an inhibitor of natural killer (NK) cell cytotoxicity (PubMed:15238421). Also acts as an ihibitor of dendritic cell maturation in an IL10-dependent manner (PubMed:16239426)

Subcellular Localization：Cell membrane; Single-pass type II membrane protein

Expression：
Tissue_specificity:It is primarily expressed in lymphoid tissues and immune cells, including natural killer (NK) cells, T cells, dendritic cells, and monocytes or macrophages (PubMed:14739280, PubMed:15238421, PubMed:15548716, PubMed:16239426, PubMed:16838277) . It can be detected (at the protein level) in macrophage-rich red pulp and lymph nodes in the spleen (PubMed:16838277) . It can also be detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes, and macrophages (PubMed:16838277) .

Induction:Down-regulated in activated leukocytes recruited to a site of inflammation

Isoforms & Post-Translational Modification：Q5QGZ9 has 5 isomers: Q5QGZ9-2: 30762 Da (predicted); Q5QGZ9-1: 32040 Da (predicted); Q5QGZ9-3: 8965 Da (predicted); Q5QGZ9-4: 27276 Da (predicted); Q5QGZ9-5: 24449 Da (predicted).
Phosphorylated at Tyr-7 by SRC in the ITIM motif following ligand-binding, promoting recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11;Highly N-glycosylated; glycosylation varies between cell types

Subunit：Homodimer; disulfide-linked (By similarity). Interacts (when the ITIM motif is phosphorylated) with PTPN6 and PTPN11 (PubMed:14739280)

Synonyms

CLL1; MICL; CLL-1; DCAL-2; C-type lectin domain family 12 member A; CD371; CD371 nanobody;

Documentación

User Guide for Antibodies (1077 KB)