DGKZ Antibody (YA1222)(PBS only)

Cat. No.: HY-P81477A: Data Sheet User Guide for Antibodies
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DGKZ Antibody (YA1222) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGKZ.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DGKZ Antibody (YA1222) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGKZ.

Host

Rabbit

Molecular Weight

Predicted band size: 104 kDa;

Observed band size: 124/114 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q13574

Gene ID

8525 [NCBI]

Immunogen

Recombinant protein of human DGKZ

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:2000-1:5000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095, PubMed:9159104). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095, PubMed:9159104). Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed:19744926, PubMed:22108654, PubMed:9159104). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed:15544348, PubMed:19744926, PubMed:22627129). The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed:15157668). Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity); Regulates RASGRP1 activity; Does not regulate RASGRP1 activity

Subcellular Localization：Nucleus; Cytoplasm, cytosol; Cell membrane; Cell projection, lamellipodium

Expression：
Tissue_specificity:Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas; Predominantly expressed in muscle

Isoforms & Post-Translational Modification：Q13574 has 7 isomers: Q13574-2: 103981 Da (predicted); Q13574-1: 124128 Da (predicted); Q13574-3: 106030 Da (predicted); Q13574-4: 104109 Da (predicted); Q13574-5: 104581 Da (predicted); Q13574-6: 101230 Da (predicted); Q13574-7: 104296 Da (predicted).
Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta

Subunit：Interacts (via PDZ-binding motif) with the PDZ domain of the syntrophin SNTG1 and that of SNX27 (PubMed:11352924, PubMed:17351151).

Research Field

Signal Transduction

Synonyms

DAGK5; DAGK6; DGK-ZETA; hDGKzeta

Documentation

User Guide for Antibodies (1077 KB)