FA2H Antibody (YA8628)

Cat. No.: HY-P88944: Data Sheet User Guide for Antibodies
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FA2H Antibody (YA8628) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FA2H.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

FA2H Antibody (YA8628) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FA2H.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 42.6 kDa

Species Reactivity

Human

SwissProt ID

Q7L5A8

Immunogen

Full length human recombinant protein of human FA2H produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the hydroxylation of free fatty acids at the C-2 position to produce 2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis (PubMed:15337768, PubMed:15863841, PubMed:17355976, PubMed:22517924). FA2H is stereospecific for the production of (R)-2-hydroxy fatty acids (PubMed:22517924). Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath (By similarity). Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier (PubMed:17355976). Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis (By similarity). Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins (By similarity)

Subcellular Localization：Endoplasmic reticulum membrane,Microsome membrane

Expression：
Tissue_Specificity: Detected in differentiating cultured keratinocytes (at protein level). Detected in epidermis and cultured keratinocytes (PubMed:17355976). Highly expressed in brain and colon. Detected at lower levels in testis, prostate, pancreas and kidney (PubMed:15337768)
Induction: Up-regulated during keratinocyte differentiation

Isoforms & Post-Translational Modification：Q7L5A8 has two isomers: Q7L5A8-1: 42791 Da (predicted); Q7L5A8-2: 18324 Da (predicted).

Synonyms

FAAH; FAH1; FAXDC1; SCS7; SPG35

Documentation

User Guide for Antibodies (1077 KB)