HBsAg Antibody (YA913)(PBS only)

Cat. No.: HY-P81076A: Data Sheet User Guide for Antibodies
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HBsAg Antibody (YA913) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to HBsAg.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

HBsAg Antibody (YA913) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to HBsAg.

Host

Mouse

Molecular Weight

Predicted band size: 44 kDa

SwissProt ID

P03141

Gene ID

944569 [NCBI]

Immunogen

HBsAg antibody was raised in mouse using highly purified hepatitis B surface antigen as the immunogen.

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgM

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein; The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid

Subcellular Localization：Virion membrane

Isoforms & Post-Translational Modification：P03141 has 3 isomers: P03141-1: 43705 Da (predicted); P03141-2: 31141 Da (predicted); P03141-3: 25394 Da (predicted).
Isoform M is N-terminally acetylated by host at a ratio of 90%, and N-glycosylated by host at the pre-S2 region;Myristoylated

Subunit：In its internal form (Li-HBsAg), interacts with the capsid protein and with the isoform S (PubMed:31700077). Interacts with host chaperone CANX (PubMed:9188622)

Synonyms

L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen; S; HBSAG_HBVC5.

Documentation

User Guide for Antibodies (1077 KB)