HMGA1 Antibody (YA1185)(PBS only)

Cat. No.: HY-P81440A: Data Sheet User Guide for Antibodies
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HMGA1 Antibody (YA1185) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGA1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

HMGA1 Antibody (YA1185) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGA1.

Host

Rabbit

Molecular Weight

Predicted band size: 12 kDa;

Observed band size: 17 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P17096

Gene ID

3159 [NCBI]

Immunogen

A synthesized peptide derived from human HMGA1

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions

Subcellular Localization：Nucleus; Chromosome

Isoforms & Post-Translational Modification：P17096 has 3 isomers: P17096-1: 11676 Da (predicted); P17096-2: 10679 Da (predicted); P17096-3: 19694 Da (predicted).
Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity;HMG-Y is not methylated;Methylation at Arg-58 is mutually exclusive with methylation at Arg-60

Subunit：Interacts with HIPK2 (By similarity)

Research Field

Microbiology

Synonyms

HMG R; HMG-I(Y); HMGA1; HMGA1A; HMGIY

Documentation

User Guide for Antibodies (1077 KB)