ICOS Antibody (YA1212)(PBS only)

Cat. No.: HY-P81467A: Data Sheet User Guide for Antibodies
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ICOS Antibody (YA1212) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ICOS.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ICOS Antibody (YA1212) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ICOS.

Host

Rabbit

Molecular Weight

Predicted band size: 23 kDa;

Observed band size: 23 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y6W8

Gene ID

29851 [NCBI]

Immunogen

A synthesized peptide derived from human ICOS

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response (PubMed:9930702). Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells (PubMed:33033255). Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection (PubMed:27135603). Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling (By similarity). In addition, activates PI3K signaling pathways independently of calcium flux (PubMed:30523347). Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Secreted

Expression：
Tissue_specificity:Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.

Induction:By phorbol myristate acetate (PMA) and ionomycin. Up-regulated early on T-cells and continues to be expressed into the later phases of T-cell activation

Isoforms & Post-Translational Modification：Q9Y6W8 has 2 isomers: Q9Y6W8-1: 22625 Da (predicted); Q9Y6W8-2: 19127 Da (predicted).
N-glycosylated

Subunit：Homodimer; disulfide-linked. Interacts with ICOSLG (PubMed:33033255). Interacts with PIK3R1 (PubMed:30523347). Interacts with TBK1; this interaction is critical for the maturation of T follicular regulatory cells (PubMed:27135603)

Research Field

Immunology

Synonyms

ICOS; AILIM; CD278; Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule

Documentation

User Guide for Antibodies (1077 KB)