intestinal alkaline phosphatase Antibody (YA9895)

(Synonyms: Alpi; Glycerophosphatase; IAP)
Customer Review

Based on 1 Customer Validation

intestinal alkaline phosphatase Antibody (YA9895) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to intestinal alkaline phosphatase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

  • Conjugation:
    Non-conjugated

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-30000

Product Details

Description

intestinal alkaline phosphatase Antibody (YA9895) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to intestinal alkaline phosphatase.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 57 kDa
Immunogen

Recombinant protein within human ALPI aa 1-503.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for intestinal alkaline phosphatase Antibody (YA9895)
    Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue using intestinal alkaline phosphatase Antibody (HY-P810633, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for intestinal alkaline phosphatase Antibody (YA9895)
    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using intestinal alkaline phosphatase Antibody (HY-P810633, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for intestinal alkaline phosphatase Antibody (YA9895)
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using intestinal alkaline phosphatase Antibody (HY-P810633, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for intestinal alkaline phosphatase Antibody (YA9895)
    Immunohistochemical analysis of paraffin-embedded mouse rectum tissue using intestinal alkaline phosphatase Antibody (HY-P810633, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for intestinal alkaline phosphatase Antibody (YA9895)
    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using intestinal alkaline phosphatase Antibody (HY-P810633, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for intestinal alkaline phosphatase Antibody (YA9895)
    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using intestinal alkaline phosphatase Antibody (HY-P810633, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for intestinal alkaline phosphatase Antibody (YA9895)
    Immunohistochemical analysis of paraffin-embedded rat colon tissue using intestinal alkaline phosphatase Antibody (HY-P810633, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for intestinal alkaline phosphatase Antibody (YA9895)
    Immunohistochemical analysis of paraffin-embedded rat duodenum tissue using intestinal alkaline phosphatase Antibody (HY-P810633, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    intestinal alkaline phosphatase is an Alkaline phosphatase that can hydrolyze various phosphate compounds

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor

  • Subunit

    Homodimer

  • SwissProt ID

    P09923

  • Gene ID
    248 [NCBI]
  • Synonyms

    Alpi; Glycerophosphatase; IAP

intestinal alkaline phosphatase Antibody (YA9895) Related Classifications

MOQ
Minimum order quantity
100 mg

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