Mad2L1 Antibody (YA7284)

Cat. No.: HY-P87600: Data Sheet User Guide for Antibodies
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Mad2L1 Antibody (YA7284) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mad2L1.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

Mad2L1 Antibody (YA7284) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mad2L1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 23 kDa;

Species Reactivity

Human

SwissProt ID

Q13257

Immunogen

Recombinant human Mad2L1 protein

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:15024386, PubMed:29162720). In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed:29162720). Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (PubMed:10700282, PubMed:11804586, PubMed:15024386)

Subcellular Localization：Nucleus,Chromosome, centromere, kinetochore,Cytoplasm,Cytoplasm, cytoskeleton, spindle pole

Isoforms & Post-Translational Modification：Q13257 has two isomers: Q13257-1: 23510 Da (predicted); Q13257-2: 10335 Da (predicted).
Phosphorylated on multiple serine residues

Subunit：Monomer and homodimer (PubMed:18022367, PubMed:18318601)

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/a549-cell.html [Content Brief]