MAPK13 (SAPK4) Antibody (YA8310)

Cat. No.: HY-P88626: Data Sheet User Guide for Antibodies
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MAPK13 (SAPK4) Antibody (YA8310) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to MAPK13 (SAPK4).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MAPK13 (SAPK4) Antibody (YA8310) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to MAPK13 (SAPK4).

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 41.9 kDa

Species Reactivity

Human, Dog, Rat, Monkey, Mouse

SwissProt ID

O15264

Immunogen

Full length human recombinant protein of human MAPK13 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells

Expression：
Tissue_Specificity: Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells

Isoforms & Post-Translational Modification：O15264 has two isomers: O15264-1: 42090 Da (predicted); O15264-2: 28779 Da (predicted).
Dually phosphorylated on Thr-180 and Tyr-182 by MAP2K3/MKK3, MAP2K4/MKK4, MAP2K6/MKK6 and MAP2K7/MKK7, which activates the enzyme

Subunit：Interacts with MAPK8IP2

Synonyms

MAPK-13; MAPK 13; p38delta; PRKM13; SAPK4

Documentation

User Guide for Antibodies (1077 KB)